Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

Yuan, Yuan; Chen, Xiaogang; Huang, Enying

doi:10.1177/1533033819886867

Cited by 17 publications

(14 citation statements)

References 41 publications

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“…This circRNA can also down-regulate expression levels of several proteins in the Wnt/b-catenin and PI3K/AKT/mTOR signal transductions in LNCaP and PC-3 cells, as representatives of androgen receptor (AR)-positive and AR-negative cells, respectively (26). miR-197 is another target of circITCH in prostate cancer cells through which it regulates progression of this type of cancer (27).…”

Section: Prostate Cancermentioning

confidence: 99%

CircITCH: A Circular RNA With Eminent Roles in the Carcinogenesis

et al. 2021

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Circular RNAs (circRNAs) are a group of long non-coding RNAs with enclosed structure generated by back-splicing events. Numerous members of these transcripts have been shown to affect carcinogenesis. Circular RNA itchy E3 ubiquitin protein ligase (circITCH) is a circRNA created from back splicing events in ITCH gene, a protein coding gene on 20q11.22 region. ITCH has a role as a catalyzer for ubiquitination through both proteolytic and non-proteolytic routes. CircITCH is involved in the pathetiology of cancers through regulation of the linear isoform as well as serving as sponge for several microRNAs, namely miR-17, miR-224, miR-214, miR-93-5p, miR-22, miR-7, miR-106a, miR-10a, miR-145, miR-421, miR-224-5p, miR-197 and miR-199a-5p. CircITCH is also involved in the modulation of Wnt/β-catenin and PTEN/PI3K/AKT pathways. Except from a single study in osteosarcoma, circITCH has been found to exert tumor suppressor role in diverse cancers. In the present manuscript, we provided a comprehensive review of investigations that reported function of circITCH in the carcinogenesis.

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Section: Prostate Cancermentioning

confidence: 99%

CircITCH: A Circular RNA With Eminent Roles in the Carcinogenesis

et al. 2021

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“…miR-17-5p, itself negatively regulates expression of HOXB13, which is known as a tumor suppressor gene being involved in development and progression of PCa. Yuan et al (78) showed the same experimental results about consequences of circ-ITCH down-regulation, although miR-197 was identified as target miRNA for circ-ITCH. Also, miR-17 was detected as target of circ-ITCH in a study by Li et al (79).…”

Section: Down-regulated Circrnas In Pcamentioning

confidence: 71%

“…Circular RNA Itchy E3 ubiquitin protein ligase (circ-ITCH) is an example for down-regulated circRNAs in PCa which has been studied in four distinct experiments (77)(78)(79). Wang et al (92) demonstrated that circ-ITCH down-regulation increases PCa cell proliferation and decreases apoptosis in vitro, while its upregulation decreases cell proliferation and in vivo tumor growth.…”

Section: Down-regulated Circrnas In Pcamentioning

confidence: 99%

The Role and Clinical Potentials of Circular RNAs in Prostate Cancer

et al. 2021

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Globally, prostate cancer (PCa) is the second most commonly diagnosed cancer in men globally. Early diagnosis may help in promoting survival in the affected patients. Circular RNAs (circRNAs) are a novel class of non-coding RNAs (ncRNAs) which have been found to show extensive dysregulation in a handful of human diseases including cancers. Progressions in RNA identification techniques have provided a vast number of circRNAs exhibiting either up-regulation or down-regulation in PCa tissues compared to normal adjacent tissues. The mechanism of action is not clear for most of dysregulated circRNAs. Among them, function of a number of newly identified dysregulated circRNAs have been assessed in PCa cells. Increase in cell proliferation, migration, invasion, and metastasis have been reported for up-regulated circRNAs which suggest their role as oncogenes. On the other hand, down-regulated circRNAs have shown tumor suppressing actions in experimental studies. Furthermore, in a majority of studies, circRNAs have been found to sponge microRNAs (miRNAs), negatively regulating expression or activity of the downstream miRNAs. Additionally, they have been identified in interaction with regulatory proteins. This axis consequently regulates a signaling pathway, a tumor suppressor, or an oncogene. Easy, quick, and reliable detection of circRNAs in human body fluids also suggests their potentials as biomarker candidates for diagnosis and prediction of prognosis in PCa patients. In this review, we have discussed the role and potentials of a number of dysregulated circRNAs in PCa.

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“…Previous studies have reported that miR-197 is significantly overexpressed in the serum of PC patients, which indicates that miR-197 plays an important role in the occurrence and development of PC. However, the molecular mechanism of miR-197 in the bone metastasis of PC has not as yet been reported ( Yuan et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, abnormal levels of miRNA expression have been found in malignant tumors, which have been found to be closely associated with the proliferation, differentiation, and apoptosis of tumor cells ( Dimakakos et al, 2014 ; Brzeszczyńska et al, 2020 ; Paul et al, 2020 ). Studies have shown that the level of miR-197 expression in the serum of patients with PC is higher than that of benign prostatic hyperplasia patients and healthy males, and the difference is statistically significant ( Yuan et al, 2019 ). These results suggest that miR-197 plays an important role in the occurrence and development of PC.…”

Section: Introductionmentioning

confidence: 99%

MiR-197 Inhibitor Loaded AbCD133@MSNs@GNR Affects the Development of Prostate Cancer Through Targeting ITGAV

Zhu

et al. 2021

Front. Cell Dev. Biol.

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Prostate cancer is one of the most severe male malignant tumors, which ranks second in mortality rate among all tumors. Traditional methods of treatment for prostate cancer produce obvious side effects and a high recurrence rate. Cancer stem cells are considered to be a group of cells that determine the proliferation, metastasis, and drug resistance of tumor. Prostate cancer therapy based on microRNAs and prostate cancer stem cells (PCSCs) has been a research hot spot in this field. Previous studies have reported that miR-197 plays an important role in the occurrence and development of prostate cancer, but the molecular mechanism of miR-197 on the development of prostate cancer has not been reported yet. In this study, we verified that miR-197 is significantly overexpressed in prostate cancer tissues and prostate cancer cells. Then, we verified that miR-197 expression affects the proliferation, invasion, and metastasis of prostate cancer cells by regulating integrin subunit alpha V (ITGAV) expression through STAT5 pathway, and the results indicated that the miR-197 inhibitor can be a prostate cancer suppressor. Then we synthesized the AbCD133@GNR@MSNs@miR-197 inhibitor drug carrier, in which 35.42 μg of the miR-197 inhibitor could be loaded in 1 mg of AbCD133@GNR@MSNs. The AbCD133@GNR@MSNs@miR-197 inhibitor demonstrated good photothermal properties and photothermal controlled-release properties. The modified CD133 antibodies on the surface of the nano drug carrier helped more drug carriers to enter the PCSCs. The pharmacodynamic effects of the AbCD133@GNR@MSNs@miR-197 inhibitor on PCSCs in vivo and in vitro were studied under near-infrared radiation. The results showed that the AbCD133@GNR@MSNs@miR-197 inhibitor prepared in this study could not only significantly suppress the development of PCSCs through ITGAV/STAT5 pathway but also significantly suppress the growth of PCSC solid tumors. In short, our study verified that miR-197 regulates the development of PCSCs through STAT5 pathway by targeting ITGAV, and the AbCD133@MSNs@GNR@miR-197 inhibitor could be a potential suppressor used in prostate cancer treatment. In short, our study found that miR-197 affected the development of prostate cancer by regulating ITGAV. The AbCD133@GNR@MSNs@miR-197 inhibitor prepared in this study could suppress the development and growth of PCSCs in vitro and in solid tumors not only by targeting the ITGAV but also through photothermal therapy. Our study not only provides a theoretical basis for the clinical treatment of prostate cancer but also provides a research scheme of drug loading and microRNA-based photothermal controlled therapy for prostate cancer.

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Upregulation of Circular RNA Itchy E3 Ubiquitin Protein Ligase Inhibits Cell Proliferation and Promotes Cell Apoptosis Through Targeting MiR-197 in Prostate Cancer

Cited by 17 publications

References 41 publications

CircITCH: A Circular RNA With Eminent Roles in the Carcinogenesis

CircITCH: A Circular RNA With Eminent Roles in the Carcinogenesis

The Role and Clinical Potentials of Circular RNAs in Prostate Cancer

MiR-197 Inhibitor Loaded AbCD133@MSNs@GNR Affects the Development of Prostate Cancer Through Targeting ITGAV

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