Upregulation of elafin expression in the fallopian tube of ectopic tubal pregnancies compared to the normal tubes

Zakizadeh, Fatemeh; Mahmoudzadeh‐Sagheb, Hamidreza; Asemi-Rad, Azam; Ghasemi, Marzieh; Moudi, Bita; Sheibak, Nadia; Asadikalameh, Zahra; Heidari, Zahra

doi:10.1016/j.jri.2020.103136

Cited by 9 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other molecules have a relevant role as an anti-inflammatory agent in the mucus of the female genital tract. Elafin (elastase-specific inhibitor) is significantly augmented in the epithelium and connective tissue in the point where the egg is implanted in tubaric interleukin [36]. Adrenomedullin is a peptide hormone with a potent anti-inflammatory activity that is highly present in the fallopian tubes and it is present in lower levels in patients diagnosed with salpingitis or a tubaric ectopic pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Ectopic pregnancies and endometrial microbiota

Aguilera

García

Quevedo

2021

Current Opinion in Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

In recent years there has been significant progress in the study of endometrial microbiota. This line of investigation has not been free of controversy and discussion. It is key for clinicians involved to remain updated with the most recent findings in microbiome and its clinical implications to be able to offer patients all possible treatments. Recent findingsThe existence of endometrial microbiota now seems undisputed. Current lines of work are centered on dysbiosis and its connection to other pathologies. It is in the field of assisted reproductive technology (ART) where this research plays an even more crucial role. In this case, we are focusing our attention towards the study of ectopic pregnancies (EP), searching for similarities in their etiopathogenesis and alterations in the endometrial microbiota.

show abstract

Section: Discussionmentioning

confidence: 99%

Ectopic pregnancies and endometrial microbiota

Aguilera

García

Quevedo

2021

Current Opinion in Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

show abstract

“…This scoring system was used to measure the survivin immunoexpression: immunohistochemistry (IHC) score (0–8) was calculated as the sum of an intensity score (0-absent, 1-weak, 2-moderate, and 3-strong) and score distribution (0: 0%, 1: < 1%, 2: 1% to 10%, 3: 10% to 33%, 4: 33% to 66%, and 5: 66% to 100%) for each tissue sample. 21…”

Section: Methodsmentioning

confidence: 99%

Survivin as a potential biomarker for early diagnosis of the progression of precancerous lesions to gastric cancer

Mahmoudzadeh-Sagheb,

Panahi,

Jami

et al. 2023

Int J Biol Markers

Self Cite

View full text Add to dashboard Cite

Background Gastric cancer is a common cancer developed in a carcinogenesis process from precancerous lesions including chronic gastritis, intestinal metaplasia, and dysplasia. Survivin, an inhibitor-of-apoptosis protein, is associated with the initiation and progression of gastric cancer. The present study aimed to evaluate the immunohistochemical expression patterns of survivin and its relationship with early diagnosis of gastric cancer in Iranian patients. Methods In this retrospective case-control study, immunoexpression of survivin was investigated on sections obtained from formalin-fixed paraffin-embedded tissue blocks of 38 chronic gastritis, 32 intestinal metaplasia, 20 dysplasia, 28 gastric adenocarcinoma, and 22 controls. Results Survivin immunoexpression in chronic gastritis was higher than controls, but this difference was not statistically significant ( P > 0.05). However, survivin immunoexpression had a steady significant increase from control and chronic gastritis to intestinal metaplasia to dysplasia to gastric adenocarcinoma ( P < 0.05). Sensitivity, specificity, and area under the curve of survivin immunohistochemical test for the diagnosis of gastric cancer were 87.5%, 74.4%, and 0.85, respectively. Males had a significantly higher survivin expression than females ( P < 0.001). Also, survivin expression was significantly higher in older patients than in younger ones ( P < 0.001). Conclusion It seems that the steady increase in survivin expression from different precancerous lesions to gastric adenocarcinoma suggests that survivin can be used as a potential biomarker for the prevention and early diagnosis of gastric cancer.

show abstract

“…The tissues were fixed in formalin, and tissue passage procedures were applied to investigate the histological changes and expression level of CB1 protein using IHC. The IHC technique was performed according to the instructions embedded in the staining kit 17‐19 . Sections were incubated with 1:200 primary antibody for CB1 antigen (Anti‐Cannabinoid Receptor I antibody, ab23703, Abcam), overnight at 4°C and for 30 min with 1:100 secondary antibody (Goat anti‐Rabbit IgG H&L Biotin secondary antibody, ab97049, Abcam).…”

Section: Methodsmentioning

confidence: 99%

Cannabinoid receptor type‐1 and its correlation with CB1 gene polymorphism‐1359G/A in ectopic pregnancy compared to the control group

Moudi

Heidari

Asemi-Rad

et al. 2021

J of Obstet and Gynaecol

Self Cite

View full text Add to dashboard Cite

Aim Ectopic pregnancy (EP) is one of the most important causes of maternal mortality. This study aimed to evaluate the immunohistochemical (IHC) expression of the cannabinoid receptor type‐1 (CB1) and its association with CB1‐1359G/A gene polymorphism (rs1049353) in the fallopian tubes in EP compared to controls. Methods In this case–control study, 100 women with EP (cases) and 100 women that underwent abdominal surgery due to the hysterectomy or uterine tubal ligation (healthy controls) were included. Genotyping of CB1‐1359G/A polymorphism, tissue expression of CB1 at the protein and mRNA levels were studied using restriction fragment length polymorphism, IHC method, and quantitative real‐time polymerase chain reaction (qRT‐PCR) analysis. Results Genotyping showed that in EP, the frequency of AA, AA+AG genotypes, and A allele was significantly higher than healthy control subjects (p = 0.001). Also, patients with EP had significantly increased IHC expression of CB‐1 compared to the control samples (p = 0.016). Patients with AA and AG genotypes had a significantly higher IHC expression of CB‐1 compared to the GG genotype. qRT‐PCR analysis showed that patients with EP had significantly increased expression of CB‐1 compared to the control samples (p < 0.001). Patients with AA and AG genotypes had higher significant mRNA expression of CB‐1 compared to the GG genotype. Conclusions CB1 is likely to be effective in creating innate immunity in humans and can affect the process of EP in the fallopian tube. CB1 is also a pathological valuable factor in identifying the pathway of inflammation during ectopic implantation.

show abstract

Upregulation of elafin expression in the fallopian tube of ectopic tubal pregnancies compared to the normal tubes

Cited by 9 publications

References 16 publications

Ectopic pregnancies and endometrial microbiota

Ectopic pregnancies and endometrial microbiota

Survivin as a potential biomarker for early diagnosis of the progression of precancerous lesions to gastric cancer

Cannabinoid receptor type‐1 and its correlation with CB1 gene polymorphism‐1359G/A in ectopic pregnancy compared to the control group

Contact Info

Product

Resources

About