Upregulation of Endothelial and Inducible Nitric Oxide Synthase Expression by Reactive Oxygen Species

Zhen, Junhui; Lu, Hua; Wang, Xiu Q.; Vaziri, Nosratola D.; Zhou, Xin J.

doi:10.1038/ajh.2007.14

Cited by 126 publications

(100 citation statements)

References 33 publications

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“…A study by Zhen et al has suggested that ROS-stimulated upregulation of eNOS expression is mediated by diminished NO availability and consequent reduction in the negative feedback regulatory action of NO on eNOS expression, which is possibly mediated by NF-κ B. 39 In our present study, PJ treatment diminished the increased eNOS activity in diabetic lung most probably through NF-κ B inactivation.…”

supporting

confidence: 62%

The Effect of Pomegranate Juice on Diabetes-Related Oxidative Stress in Rat Lung

Çukurova¹,

Hergünsel²,

Eren³

et al. 2012

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : It is known that diabetes leads to depletion of the cellular antioxidant defense system and this causes the organ injuries related to the diabetic complications. Antioxidants have been shown to reduce indices of oxidative stress measures in experimental disease models and in humans. The present study was designed to evaluate the oxidative stress in lungs as well as the therapeutic effect of pomegranate juice (PJ), as an antioxidant, in rats with streptozotocin-induced diabetes. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Twenty seven rats were enrolled in the study where diabetes was induced by streptozotocin injection. Rats were treated with either PJ or saline for 10 weeks, and their lungs were harvested for histologic and immunohistochemical evaluation at the end. Protein carbonyl content (PCC), sialic acid (SA), superoxide dismutase (SOD) and reduced glutathione (GSH) activities were measured in the pulmonary tissue as well as the presence of endothelial nitric oxide synthase (eNOS), through immunohistochemistry. R Re es su ul lt ts s: : The pulmonary tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. There was increased eNOS expressions in diabetic lungs, but PJ treatment diminished both eNOS expressions and inflammatory changes in pulmonary tissue. PCC and SA levels were higher in diabetic lungs (p<0.01) whereas SOD was lower (p=0.021) and GSH did not change significantly in comparison to PJ-treated diabetic group (p=0.374). C Co on nc cl lu us si io on n: : The PJ treatment effectively reduced the oxidative stress and contributed to tissue recovery in experimental diabetic lungs. These findings would be of clinical relevance.K Ke ey y W Wo or rd ds s: : Oxidative stress; diabetes complications; lung; models, animal; punicaceae Ö ÖZ ZE ET T A Am ma aç ç: : Diyabetin hücresel antioksidan savunma sisteminde azalmaya yol açtığı bilinmektedir ve bu da diabetik komplikasyonlara bağlı organ hasarına neden olur. Antioksidanların oksidatif stres ölçütleri göstergelerini azalttığı deneysel hastalık modellerinde ve insanlarda gösterilmiştir. Bu çalışma akciğerlerdeki oksidatif stresi ve streptozotosinle indüklenmiş diyabeti olan sıçanlarda, bir antioksidan olan nar suyunun tedavi edici etkisini değerlendirmek için tasarlandı. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Streptozotosin enjeksiyonu ile diyabetin indüklendiği 27 sıçan çalışmaya alındı. Sıçanlar 10 hafta boyunca nar suyu veya serum fizyoojikle tedavi edildi ve bitiminde histolojik ve immünohistokimyasal değerlendirmeler için akciğerleri toplandı. Akciğer dokusunda protein karbonil içeriği (PCC), sialik asit (SA), süperoksit dizmutaz (SOD) ve redükte glutatyon (GSH) aktiviteleri ve endotelyal nitrik oksit sentaz (eNOS) varlığı immünohistokimyasal olarak ölçüldü. B Bu ul lg gu ul la ar r: : Diyabetik sıçanlarda akciğer dokusu streptozotosin uygulamasına bağlı oksidatif değişiklikler gösterdi. Diyabetik akciğerlerde artmış eNOS ekspre...

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supporting

confidence: 62%

The Effect of Pomegranate Juice on Diabetes-Related Oxidative Stress in Rat Lung

Çukurova¹,

Hergünsel²,

Eren³

et al. 2012

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

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“…Zhen and colleagues 52 showed apparent suppression of eNOS activity in human coronary artery endothelial cells and rats by the NO donor S-Nitroso-N-Acetyl-D,LPenicillamine (SNAP). This suggests NO exerts a negative feedback effect on eNOS expression and activity.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary nitrate on blood pressure, endothelial function, and insulin sensitivity in type 2 diabetes

Gilchrist

Winyard

Aizawa

et al. 2013

Free Radical Biology and Medicine

222

209

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Diets rich in green, leafy vegetables have been shown to lower BP and reduce the risk of cardiovascular disease. Green, leafy vegetables and beetroots are particularly rich in inorganic nitrate. Dietary nitrate supplementation, via sequential reduction to nitrite and NO, has previously been shown to lower BP and improve endothelial function in healthy humans.We sought to determine if supplementing dietary nitrate with beetroot juice, a rich source of nitrate, will lower BP, improve endothelial function and insulin sensitivity in individuals with type 2 diabetes (T2DM). Twenty-seven patients, age 67.2 +/-4.9 years, (18 male) were recruited for a double blind, randomised, placebo-controlled crossover trial. Participants were randomised to begin in either order a 2 week period of supplementation with 250 ml beetroot juice daily (active) or 250 ml nitratedepleted beetroot juice (placebo). At the conclusion of each intervention period 24 hour ambulatory blood pressure monitoring, tests of macro and microvascular endothelial function and a hyperinsulinaemic isoglycaemic clamp were performed. After two weeks administration of beetroot juice mean ambulatory systolic BP was unchanged: 134.6 ± 8.4 mmHg versus 135.1 ± 7.8 mmHg (mean ± SD) placebo vs. active -mean difference of -0.5 mmHg (placebo-active), p=0.737 (95% CI -3.9 to 2.8). There were no changes in macrovascular or microvascular endothelial function or insulin sensitivity. Supplementation of the diet with 7.5 mmoles of nitrate per day for 2 weeks caused an increase in plasma nitrite and nitrate concentration, but did not lower BP, improve endothelial function or insulin sensitivity in individuals with T2DM.3

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“…In the presence of ROS/RNS, the inducible isoform of NOS (iNOS; coded by NOS2A) is upregulated by nuclear factor kappa B in the liver. 22,23 Due to the biological imperative of protecting the cellular environment against oxidative stress, ROS/RNS are rapidly eliminated by antioxidant enzymes such as GSTs, NAD(P)H dehydrogenase quinone 1 (NQO1; coded by NQO1), and heme oxygenase 1 (HO1; coded by HMOX1) in humans. 24 The expression of these genes, which carry an antioxidant-responsive element (ARE) in their promoter regions is regulated by several transcriptional factors, including nuclear factor erythroid 2-related factor 2 (Nrf2; coded by NFE2L2), BTB and CNC homology 1 (Bach1; coded by BACH1), and the small Maf basic leucine zipper proteins (MafF, MafG, and MafK).…”

Section: Introductionmentioning

confidence: 99%

Genetic variants in antioxidant pathway: Risk factors for hepatotoxicity in tuberculosis patients

et al. 2012

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SummaryTuberculosis (TB) treatment can cause serious sequelae including adverse effects such as anti-TB drug-induced hepatotoxicity (ATDH). We performed a candidate gene-based association study between single nucleotide polymorphisms (SNPs) in 10 genes in the antioxidant pathway and ATDH susceptibility. The subjects comprised 100 Japanese patients with pulmonary TB who received a treatment regimen including isoniazid and rifampicin. Out of them, 18 patients had ATDH. Thirty-four tag SNPs in 10 genes were analyzed by PCR-restriction fragment length polymorphism or PCR-direct DNA sequencing. The frequencies of alleles and genotypes between patients with and without ATDH were compared in three different genetic models. Statistical analyses revealed that a C/C genotype at rs11080344 in NOS2A, a C/C genotype at rs2070401 in

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Upregulation of Endothelial and Inducible Nitric Oxide Synthase Expression by Reactive Oxygen Species

Cited by 126 publications

References 33 publications

The Effect of Pomegranate Juice on Diabetes-Related Oxidative Stress in Rat Lung

The Effect of Pomegranate Juice on Diabetes-Related Oxidative Stress in Rat Lung

Effect of dietary nitrate on blood pressure, endothelial function, and insulin sensitivity in type 2 diabetes

Genetic variants in antioxidant pathway: Risk factors for hepatotoxicity in tuberculosis patients

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