Upregulation of formyl-peptide and interleukin-8—induced eosinophil chemotaxis in patients with allergic asthma

Warringa, R. A. J.; Mengelers, H. J. J.; Raaijmakers, J. A. M.; Bruijnzeel, P. L. B.; Koenderman, Leo

doi:10.1016/0091-6749(93)90323-8

Cited by 110 publications

(70 citation statements)

References 36 publications

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“…Such activation contrasts with the model for the recruitment of leukocytes (16), including eosinophils (20), in which integrin activation is assumed to occur locally and concurrently with rolling and tethering on endothelium. Interestingly, bone marrow-derived progenitor cells or circulating eosinophils of asthmatics have been shown to be activated upon Ag challenge also as measured by the up-regulation of IL-5 receptor ␣ and CCR3, the activation of CD32, and greater responsiveness to chemoattractants (42,(81)(82)(83). Further, eosinophils from allergic asthmatics have been shown to have a greater capacity to adhere to and transmigrate through endothelium than eosinophils from normal donors (51,84).…”

Section: Discussionmentioning

confidence: 99%

Up-Regulation and Activation of Eosinophil Integrins in Blood and Airway after Segmental Lung Antigen Challenge

Johansson¹,

Kelly

Busse

et al. 2008

The Journal of Immunology

117

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We hypothesized that there are clinically relevant differences in eosinophil integrin expression and activation in patients with asthma. To evaluate this, surface densities and activation states of integrins on eosinophils in blood and bronchoalveolar lavage (BAL) of 19 asthmatic subjects were studied before and 48 h after segmental Ag challenge. At 48 h, there was increased expression of αD and the N29 epitope of activated β1 integrins on blood eosinophils and of αM, β2, and the mAb24 epitope of activated β2 integrins on airway eosinophils. Changes correlated with the late-phase fall in forced expiratory volume in 1 s (FEV1) after whole-lung inhalation of the Ag that was subsequently used in segmental challenge and were greater in subjects defined as dual responders. Increased surface densities of αM and β2 and activation of β2 on airway eosinophils correlated with the concentration of IL-5 in BAL fluid. Activation of β1 and β2 on airway eosinophils correlated with eosinophil percentage in BAL. Thus, eosinophils respond to an allergic stimulus by activation of integrins in a sequence that likely promotes eosinophilic inflammation of the airway. Before challenge, β1 and β2 integrins of circulating eosinophils are in low-activation conformations and αDβ2 surface expression is low. After Ag challenge, circulating eosinophils adopt a phenotype with activated β1 integrins and up-regulated αDβ2, changes that are predicted to facilitate eosinophil arrest on VCAM-1 in bronchial vessels. Finally, eosinophils present in IL-5-rich airway fluid have a hyperadhesive phenotype associated with increased surface expression of αMβ2 and activation of β2 integrins.

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Section: Discussionmentioning

confidence: 99%

Up-Regulation and Activation of Eosinophil Integrins in Blood and Airway after Segmental Lung Antigen Challenge

Johansson¹,

Kelly

Busse

et al. 2008

The Journal of Immunology

117

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“…Taken together, these observations suggest that LTB 4 mediates Aginduced increases in BAL IL-8 and the blockade of this pathway by CP-105,696 may have further contributed to the protective effects of this compound. IL-8 is a potent chemoattractant and activator of neutrophils in primates (21) and purportedly induces eosinophil chemotaxis in human asthmatics (22). Furthermore, intradermal administration of IL-8 in rabbits causes plasma exudation (23).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo effects of leukotriene B4 antagonism in a primate model of asthma.

Turner¹,

Breslow²,

Conklyn³

et al. 1996

J. Clin. Invest.

104

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“…Expression of IL-4 and -5, which are T-helper cell (Th) type 2-derived cytokines associated with eosinophilia of asthma, has been reported in plasma cells associated with submucosal glands [30]. IL-8, in addition to its neutrophil chemotactic effects, also has eosinophil chemotactic properties [31]. During exacerbations of chronic bronchitis, a marked increase in expression of RANTES (regulated on activation, normal T-cell expressed and secreted) has been reported in epithelium and subepithelium associated with a marked increase in submucosal eosinophil numbers [32].…”

Section: Eosinophilsmentioning

confidence: 99%

Multifaceted mechanisms in COPD: inflammation, immunity, and tissue repair and destruction

Chung¹,

Adcock²

2008

Eur Respir J

518

425

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Chronic obstructive pulmonary disease is a leading global cause of morbidity and mortality that is characterised by inexorable deterioration of small airways obstruction with emphysema associated with cellular inflammation and structural remodelling. Other features include apoptosis as well as proliferation of cells, and both tissue repair and lack of tissue repair.Metalloprotease release, together with that of apoptotic factors, may underlie the emphysema, and, conversely, fibrosis of the small airways may be accounted for by the effects of growth factor activation. In advanced disease, influential factors include the development of autoimmunity, with activation of dendritic cells and T-helper cells of both type 1 and 2, and the senescence response.An inability of macrophages to ingest apoptosed cells and bacteria may exacerbate inflammatory responses. Systemic inflammation with concomitant cardiovascular disease and metabolic syndrome may reflect the effect of cigarette smoke on nonpulmonary cells. Corticosteroid resistance may be secondary to oxidative stress mechanisms, such as inactivation of histone deacetylases.The mechanisms of chronic obstructive pulmonary disease may be heterogeneous, according to severity, and clinical phenotypes need to be correlated with cellular and pathological processes. Treatments may be targeted to patients with specific mechanisms.

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Upregulation of formyl-peptide and interleukin-8—induced eosinophil chemotaxis in patients with allergic asthma

Cited by 110 publications

References 36 publications

Up-Regulation and Activation of Eosinophil Integrins in Blood and Airway after Segmental Lung Antigen Challenge

Up-Regulation and Activation of Eosinophil Integrins in Blood and Airway after Segmental Lung Antigen Challenge

In vitro and in vivo effects of leukotriene B4 antagonism in a primate model of asthma.

Multifaceted mechanisms in COPD: inflammation, immunity, and tissue repair and destruction

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