Upregulation of Hypoxia-Inducible Factor-1α mRNA and its Clinical Significance in Non-small Cell Lung Cancer

Abstract

The expression of HIF-1alpha mRNA is associated with disease progression in NSCLC tissues, and is expected as a biomarker or therapeutic target.

Improved vascular organization enhances functional integration of engineered skeletal muscle grafts

“…Nevertheless, a number of groups reported the correlation between increased mRNA and increased protein expression of Hif1α that also correlated to increased vascular density. Hif1α increased levels also correlated to increased VEGF levels that, as mentioned earlier, correlate to increased vascular density (43)(44)(45)(46)(47)(48)(49)(50)(51). We also show this pattern in our gene expression research which correlates to the increased vascular density, as observed in the grafts.…”

Early Growth Response-1 Induces and Enhances Vascular Endothelial Growth Factor-A Expression in Lung Cancer Cells

“…57,58 However, a large proportion of human tumors exhibit VEGF-A expression in the absence of HIF-1␣. 39 Although the VEGF-A promoter contains a HIF-1␣ binding site, 58 our study demonstrated negligible HIF-1␣ expression in lung cancer cells cultivated under ordinary conditions, and luciferase reporter assays revealed no significant association between the HIF-1␣ binding site and VEGF-A promoter activity. However, we also demonstrated here that HIF-1␣ was induced in lung cancer cells cultivated under hypoxic conditions and that EGR-1 expression enhanced HIF-1␣-induced VEGF-A expression.…”

The HIF-2α-Driven Pseudo-Hypoxic Phenotype in Tumor Aggressiveness, Differentiation, and Vascularization

“…Both HIF-1α and HIF-2α are frequently expressed in NSCLC, also during early progression of disease; but whereas HIF-2α causes, or is a surrogate marker for poor clinical prognosis (Giatromanolaki et al 2001), the role of HIF-1α in predicting outcome is debated. Some reports demonstrate that HIF-1α expression has no impact on patient overall survival (Giatromanolaki et al 2001;Kim et al 2005), but potentially contradictory data exist (Volm and Koomagi 2000;Yohena et al 2009). …”