Upregulation of lncRNA ADAMTS9-AS2 Promotes Salivary Adenoid Cystic Carcinoma Metastasis via PI3K/Akt and MEK/Erk Signaling

Xie, Shuhua; Yu, Xin; Li, Yingru; Ma, Hanyu; Fan, ST; Chen, Weixiong; Pan, Guokai; Wang, Weiwei; Zhang, Hanqing; Li, Jinsong; Lin, Zhaoyu

doi:10.1016/j.ymthe.2018.08.018

Cited by 67 publications

(64 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[25][26][27][28][29] And abnormal expression of PI3K-Akt signaling pathway has been found to play an important role in the diagnosis and prognosis of gastric cancer. In recent years, a large number of studies have found that the PI3K-Akt signaling pathway is involved in important functions such as growth, differentiation, proliferation, invasion, and metastasis of gastric cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…In gliomas, ADAMTS9-AS2 acts as a novel tumor suppressor that inhibits glioma cell metastasis. [25][26][27] The PI3K-Akt signaling pathway is involved in important functions such as growth, differentiation, invasion, and metastasis of gastric cancer cells. 23 In ovarian cancer, ADAMTS9-AS2 affects the progression of ovarian cancer by targeting the miR-182-5p/FOXF2 signaling pathway.…”

Section: Relationship Between Lncrnamentioning

confidence: 99%

See 1 more Smart Citation

Identification of the complex regulatory relationships related to gastric cancer from lncRNA‐miRNA‐mRNA network

Wang

Ding

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

The oncogenesis and progression of gastric cancer are closely correlated with the complex regulatory relationships among messenger RNA (mRNA), long noncoding RNA (lncRNA), and microRNA (miRNA). After constructing the gastric cancer lncRNA‐miRNA‐mRNA regulatory network, we analyzed the network topology properties and found that lncRNA ADAMTS9‐AS2 and C20orf166‐AS1 and miRNA hsa‐mir‐204 are key nodes. Further functional enrichment analysis and survival analysis were performed on these key nodes and the RNAs interacting with them. We found that CHRM2, ANGPT2, and COL1A1 interacting with ADAMTS9‐AS2 are enriched in the PI3K‐Akt signaling pathway, and low expression of the ADAMTS9‐AS2 is closely related to the prognosis of patients. Abnormal expression of CACNA1H, FLNA, and FLNC interacting with lncRNA C20orf166‐AS1 is associated with MAPK signaling pathway in gastric cancer. In addition, the downregulated miRNA hsa‐mir‐204 promotes invasion and proliferation of gastric cancer cells by regulating the abnormal expression of mRNAs (CHRDL1 and NPTX1) and lncRNAs (ADAMTS9‐AS2, NKX2‐1‐AS1, TLR8‐AS1, and VCAN‐AS1). This study systematically analyzed the lncRNA‐miRNA‐mRNA regulatory network of gastric cancer, which not only has a new understanding of the pathogenesis of gastric cancer, but also provides new insights for the early diagnosis and treatment of gastric cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Relationship Between Lncrnamentioning

confidence: 99%

Identification of the complex regulatory relationships related to gastric cancer from lncRNA‐miRNA‐mRNA network

Wang

Ding

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…lncRNAs located in the cytoplasm are usually associated with post-transcriptional regulation. 20,26 To identify DANCR-interacting proteins in BCa cells, we performed RNA pull-down assays using in vitro-transcribed biotinylated DANCR. One overtly differential band between 130 and 150 kDa appeared after silver staining, and it was identified as LRPPRC by mass spectrometry ( Figure 4C; Figure S3A).…”

Section: Dancr Directly Interacts With Lrpprc To Play Key Roles In Bcamentioning

confidence: 99%

DANCR Promotes Metastasis and Proliferation in Bladder Cancer Cells by Enhancing IL-11-STAT3 Signaling and CCND1 Expression

et al. 2019

View full text Add to dashboard Cite

The prognosis for patients with bladder cancer (BCa) with lymph node (LN) metastasis is poor, and it is not improved by current treatments. Long noncoding RNAs (lncRNAs) are involved in the pathology of various tumors, including BCa. However, the role of Differentiation antagonizing non-protein coding RNA (DANCR) in BCa LN metastasis remains unclear. In this study, we discover that DANCR was significantly upregulated in BCa tissues and cases with LN metastasis. DANCR expression was positively correlated with LN metastasis status, tumor stage, histological grade, and poor patient prognosis. Functional assays demonstrated that DANCR promoted BCa cell migration, invasion, and proliferation in vitro and enhanced tumor LN metastasis and growth in vivo. Mechanistic investigations revealed that DANCR activated IL-11-STAT3 signaling and increased cyclin D1 and PLAU expression via guiding leucine-rich pentatricopeptide repeat containing (LRPPRC) to stabilize mRNA. Moreover, oncogenesis facilitated by DANCR was attenuated by anti-IL-11 antibody or a STAT3 inhibitor (BP-1-102). In conclusion, our findings indicate that DANCR induces BCa LN metastasis and proliferation via an LRPPRC-mediated mRNA stabilization mechanism. DANCR may serve as a multi-potency target for clinical intervention in LN-metastatic BCa.

show abstract

“…The PARIS Kit purchased from Invitrogen (Carlsbad, CA, USA) was applied to isolate cytoplasmic and nuclear RNAs using a previously established protocol, followed by RT-qPCR detection [15]. Total RNA from OSCC tissues and cells was collected using the TRIzol Reagent (Invitrogen Life Technologies) and was subsequently reverse transcribed to cDNA using the PrimeScript RT reagent Kit (Takara, Tokyo, Japan).…”

Section: Subcellular Fraction and Real-time Quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

Liu

Jian

et al. 2020

BMC Cancer

View full text Add to dashboard Cite

Background: Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) play an important role in tumor progression. However, the potential biological functions and clinical importance of Linc01234 in oral squamous cell carcinoma (OSCC) remain unclear. Methods: We evaluated the expression profile and prognostic value of Linc01234 in OSCC tissues by RT-qPCR. Then, functional in vitro experiments were performed to investigate the effects of Linc01234 on tumor growth, migration and invasion in OSCC. Mechanistically, RT-qPCR, bioinformatic analysis and dual luciferase reporter assays were performed to identify a competitive endogenous RNA (ceRNA) mechanism involving Linc01234, miR-433-3p and PAK4. Results: We found that Linc01234 was clearly upregulated in OSCC tissues and cell lines, and its level was positively associated with T stage, lymph node metastasis, differentiation and poor prognosis of patients with OSCC. Our results shown that Linc01234 inhibited cell proliferation and metastatic abilities in CAL27 and SCC25 cells following its knockdown. Mechanistic analysis indicated that Linc01234 may act as a ceRNA (competing endogenous RNA) of miR-433-3p to relieve the repressive effect of miR-433-3p on its target PAK4. Conclusions:Our results indicated that Linc01234 promotes OSCC progression through the Linc01234/miR-433/ PAK4 axis and might be a potential therapeutic target for OSCC.

show abstract

Upregulation of lncRNA ADAMTS9-AS2 Promotes Salivary Adenoid Cystic Carcinoma Metastasis via PI3K/Akt and MEK/Erk Signaling

Cited by 67 publications

References 41 publications

Identification of the complex regulatory relationships related to gastric cancer from lncRNA‐miRNA‐mRNA network

Identification of the complex regulatory relationships related to gastric cancer from lncRNA‐miRNA‐mRNA network

DANCR Promotes Metastasis and Proliferation in Bladder Cancer Cells by Enhancing IL-11-STAT3 Signaling and CCND1 Expression

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

Contact Info

Product

Resources

About