Upregulation of miR-513b inhibits cell proliferation, migration, and promotes apoptosis by targeting high mobility group-box 3 protein in gastric cancer

Chen, Xudong; Wang, Fuqing; Gao, Fenglan; Luo, Hailan; Wang, Yuanyuan; Du, Yuwen; Chen, Xiaonan; Xue, Changgui; Dong, Ziming; Song, Guohua

doi:10.1007/s13277-014-2405-z

Cited by 35 publications

(29 citation statements)

References 27 publications

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“…MiR-BART8-3p was highly expressed in our study, and it has been demonstrated to share seed sequences with miR-513b (Chen et al 2010). High mobility group-box 3 (HMGB3) is a potential target for miR-513b, which is an oncogene for leukemia and can promote cell proliferation (Chen et al 2014). It was speculated that miR-BART8-3p may act as a competitor to suppress the expression of miR-513b.…”

Section: Discussionmentioning

confidence: 62%

“…When miR-513b is down-regulated, HMGB3 will be overexpressed. The elevated HMGB3 could promote EBVinfected CD8 + T cell proliferation, contributing to abnormal EBV-infected CD8 + T cell activation (Chen et al 2014). We propose that EBV-miRNA might influence immune functions of EBV-infected CD8 + T cells by sharing seed sequences with cellular miRNAs.…”

Section: Discussionmentioning

confidence: 91%

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Profiling of EBV-Encoded microRNAs in EBV-Associated Hemophagocytic Lymphohistiocytosis

Zhou

Xie

Gao

et al. 2015

Tohoku J. Exp. Med.

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Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a life-threatening complication of EBV infection. MicroRNAs (miRNAs) were small non-coding RNA, and EBV could encode miRNAs that are involved in the progression of infection. However, the profiles of EBV-miRNAs in EBV-HLH were unknown. Here, we aimed to profile the expression of EBV-miRNAs in children with EBV-HLH by analyzing 44 known EBV-miRNAs, encoded within the BamHI fragment H rightward open reading frame 1 (BHRF1) and the BamHI-A region rightward transcript (BART), in plasma and cellular targets by real-time quantitative PCR. The study included 15 children with EBV-HLH, 15 children with infectious mononucleosis (IM), and 15 healthy controls. CD8 + T cells were found to be the cellular target of EBV infection in EBV-HLH, while CD19 + B cells were infected with EBV in IM. We also found the greater levels of several miRNAs encoded by BART in EBV-HLH, compared to those in IM and healthy controls, whereas the levels of BHRF1 miRNAs were lower than those in IM. The profile and pattern of EBV-miRNAs in EBV-HLH indicated that EBV could display type II latency in EBV-HLH. Importantly, the level of plasma miR-BART16-1 continued decreasing during the whole chemotherapy, suggesting that plasma miR-BART16-1 could be a potential biomarker for monitoring EBV-HLH progression. The pathogenesis of EBV-HLH might be attributed to the abundance of EBV-miRNAs in EBV-HLH. These findings help elucidate the roles of EBV miRNAs in EBV-HLH, enabling the understanding of the basis of this disease and providing clues for its treatment.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 91%

Profiling of EBV-Encoded microRNAs in EBV-Associated Hemophagocytic Lymphohistiocytosis

Zhou

Xie

Gao

et al. 2015

Tohoku J. Exp. Med.

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“…Luciferase activity was measured by the Dual-Luciferase Reporter Assay System (Promega). The ratios of TOP/FOP were calculated and used as indicators of WNT signaling activity [22]. …”

Section: Methodsmentioning

confidence: 99%

HMGB3 promotes growth and migration in colorectal cancer by regulating WNT/β-catenin pathway

et al. 2017

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Colorectal cancer (CRC) is the third leading cause of cancer-related deaths and a major health problem. High mobility group box 3 (HMGB3), a member of the high-mobility group box (HMGB) family, was reported to be over-expressed in gastric carcinoma and bladder cancer. However, the function of HMGB3 in CRC remains unclear. Here, we found that HMGB3 was up-regulated in CRC at both mRNA and protein levels. qRT-PCR results showed that high expression of HMGB3 had positive correlation with serosal invasion, lymph metastasis, and tumor–node–metastasis (TNM) stage in CRC patient. Functional experiments showed that HMGB3 can promote CRC cells proliferation and migration in vitro. Moreover, we found HMGB3 can active WNT/β-catenin pathway to increase the expression level of c-Myc and MMP7. These results may be the reason for HMGB3 oncogene role in CRC. In summary, our data indicated that HMGB3 may serve as an oncoprotein and could be used as a potential prognostic marker in CRC.

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“…Another miRNA, miRNA-513b-5p, inhibits cell proliferation and migration and promotes apoptosis by targeting high-mobility group-box 3 protein (HMGB3) in gastric cancer (Chen et al 2014). With regard to PCOS, the apoptosis level is increased in the granulosa cells (Onalan et al 2005, Lin et al 2012; thus, we considered that the up-regulated miRNA-513b-5p in the PCOS cumulus cells might participate in the regulation of apoptosis with an impact on the atretic process of the follicles, leading to follicle maturation disorder.…”

Section: Discussionmentioning

confidence: 99%

Identification of altered microRNAs and mRNAs in the cumulus cells of PCOS patients: miRNA-509-3p promotes oestradiol secretion by targeting MAP3K8

Huang¹,

Liu²,

Hao³

et al. 2016

Reproduction

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Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women and is characterised by polycystic ovaries, hyperandrogenism and chronic anovulation. Although the clinical and biochemical signs of PCOS are typically heterogeneous, abnormal folliculogenesis is considered a common characteristic of PCOS. Our aim is to identify the altered miRNA and mRNA expression profiles in the cumulus cells of PCOS patients to investigate their molecular function in the aetiology and pathophysiology of PCOS. In this study, the miRNA expression profiles of the cumulus cell samples isolated from five PCOS and five control patients were determined by an miRNA microarray. At the same time, the altered mRNA profiles of the same cumulus cell samples were also identified by a cDNA microarray. From the microarray data, 17 miRNAs and 1263 mRNAs showed significantly different expression in the PCOS cumulus cells. The differentially expressed miRNA-509-3p and its potential target gene (MAP3K8) were identified from the miRNA and mRNA microarrays respectively. The expression of miRNA-509-3p was up-regulated and MAP3K8 was down-regulated in the PCOS cumulus cells. The direct interaction between miRNA-509-3p and MAP3K8 was confirmed by a luciferase activity assay in KGN cells. In addition, miRNA-509-3p mimics or inhibitor transfection tests in KGN cells further confirmed that miRNA-509-3p improved oestradiol (E 2 ) secretion by inhibiting the expression of MAP3K8. These results help to characterise the pathogenesis of anovulation in PCOS, especially the regulation of E 2 production.

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Upregulation of miR-513b inhibits cell proliferation, migration, and promotes apoptosis by targeting high mobility group-box 3 protein in gastric cancer

Cited by 35 publications

References 27 publications

Profiling of EBV-Encoded microRNAs in EBV-Associated Hemophagocytic Lymphohistiocytosis

Profiling of EBV-Encoded microRNAs in EBV-Associated Hemophagocytic Lymphohistiocytosis

HMGB3 promotes growth and migration in colorectal cancer by regulating WNT/β-catenin pathway

Identification of altered microRNAs and mRNAs in the cumulus cells of PCOS patients: miRNA-509-3p promotes oestradiol secretion by targeting MAP3K8

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