Upregulation of PUM1 Expression in Preeclampsia Impairs Trophoblast Invasion by Negatively Regulating the Expression of the lncRNA HOTAIR

Zhang, Yan; He, Xiaoyun; Shi, Qin; Mo, Hui-Qin; Li, Xiao; Wu, Fan; Zhang, Jing; Li, Xing; Mao, Lin; Peng, Ya-Qing; Guo, Yuna; Lin, Yi; Tian, Fu‐Ju

doi:10.1016/j.ymthe.2019.11.025

Cited by 40 publications

(31 citation statements)

References 41 publications

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“…In our study, we mainly focused on detecting TGF‐β1 expressed in DSCs and trophoblasts. We did not further distinguish the subsets of trophoblasts due to the low proportion of EVT 86‐90 . Trophoblasts also can produce a similar level of TGF‐β1 as DSCs but failed to upregulate the expression of CD103 on CD69 + CD8 + T cells.…”

Section: Discussionmentioning

confidence: 96%

Tissue‐resident CD8⁺T memory cells with unique properties are present in human decidua during early pregnancy

Huang

Liu

et al. 2020

American J Rep Immunol

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Problem Resident memory T (TRM) cells reside in the uterus during pregnancy may play an important role in balancing maternal‐fetal tolerance with anti‐infectious immunity. Although CD8+TRM and decidual CD8+T cells have been extensively characterized, the properties of decidual CD8+TRM (dTRM) cells remain poorly defined. Method of study We investigated the heterogeneity, phenotypes, and functions of dTRM cells, and compared the proportion of dTRM cells between normal pregnancy and recurrent spontaneous abortion (RSA) using flow cytometry. Moreover, we cocultured peripheral CD8+T (CD8+pT) cells with trophoblast, or decidual stomal cells (DSCs) in the presence or absence of anti‐TGF‐β antibody or TGF‐β type I receptor inhibitor to explore the effects of maternal‐fetal environment on decidual CD8+TRM cell formation. Results We found that CD69+CD103+TRM cells were abundant in CD8+dT cells but not in CD4+dT cells with effector‐memory (EM, CD45RA−CCR7−) phenotypes. The percentage of dTRM cells from RSA patients was significantly higher than that from normal pregnancy. Furthermore, dTRM cells showed increased expressions of chemokine receptors, T‐cell exhaustion‐related molecules, and produced more anti‐inflammatory cytokines and effector cytokines upon stimulation. Moreover, DSCs produced a considerable level of TGF‐β and upregulated CD103 expression on CD69+CD8+pT cells, which can be significantly reversed by blocking TGF‐β receptor. Conclusion Our findings demonstrate that TRM cells with unique properties are present in the decidua during human early pregnancy. They possess an enhanced capacity to produce effector cytokines and regulatory molecules, which might be important in the balance between maternal‐fetal immune tolerance and the capacity to aggressively respond to infections.

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Section: Discussionmentioning

confidence: 96%

Tissue‐resident CD8⁺T memory cells with unique properties are present in human decidua during early pregnancy

Huang

Liu

et al. 2020

American J Rep Immunol

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“…41,43 Trophoblast proliferation, migration and invasion were also found to be regulated by intracellular proteins involved in extracellular signaling and gene-like eukaryotic translation initiation factors 5A1 (EIF5A1), annexinA7 (ANXA7) and PUM homolog 1 (PUM1). [44][45][46] Zhang et al reported that PUM1 was highly expressed in the placental villi of patients with PE by decreasing the half-life and steady-state level of long noncoding RNA (lncRNA) HOTAIR; PUM1 overexpression and knockdown regulated the invasion and proliferation of trophoblasts. 46 Herein, knocking down PLGF expression led to decreased migration and invasion of trophoblasts; these effects were recovered by overexpression of PLGF.…”

Section: Discussionmentioning

confidence: 99%

“…[44][45][46] Zhang et al reported that PUM1 was highly expressed in the placental villi of patients with PE by decreasing the half-life and steady-state level of long noncoding RNA (lncRNA) HOTAIR; PUM1 overexpression and knockdown regulated the invasion and proliferation of trophoblasts. 46 Herein, knocking down PLGF expression led to decreased migration and invasion of trophoblasts; these effects were recovered by overexpression of PLGF. Nevertheless, PLGF is a widely used biomarker in diagnosing PE.…”

Section: Discussionmentioning

confidence: 99%

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

Tao

Xia

Chen

et al. 2020

J of Obstet and Gynaecol

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Aim This study aimed to investigate the effect of high glucose (HG) level on the proliferation, migration and invasion of trophoblasts and determine the role of placental growth factor (PLGF) in the process. Methods HTR8‐S/Vneo was treated with different concentrations of d‐glucose (0, 10, 15, 20, 25 and 30 μM) at different times (0, 6, 12 and 24 h). qRT‐PCR and Western blot analyses were used to measure PLGF expression. The protein level of PLGF was measured by immunofluorescence. Cell proliferation was assessed with CCK‐8 analysis. Wound healing and transwell assays were used to evaluate cell migration and invasion. Intercellular ROS was detected with DCFH‐DA. Results After d‐glucose treatment, the viability decreased in 25 and 30 μM groups. The HG group (25 μM) showed inhibited cell migration and invasion ability. The mRNA and protein levels of PLGF decreased under HG condition. Elevated ROS production was also detected in the HG group. Knocked‐down PLGF expression enhanced increased ROS production and decreased cell migration and invasion, which reverted to the original levels after PLGF was overexpressed. Conclusion High glucose treatment inhibited HTR8‐S/Vneo viability, migration and invasion by downregulating PLGF expression.

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“…A previous microarrayseq identified CXCL10 and CXCL11 were functionally important transcriptional target genes of Eda involved in hair follicle spacing (Lefebvre et al, 2012). Other DEG, such as FOS, NOTCH2 and NOTCH3, were also found related to follicle hair cycling, take part in MAPK, and notch signaling pathways (Miao et al, 2018;Zhang et al, 2020a;Zhang et al, 2020b). Above all, we systematically identified the lncRNAs and mRNAs involved in Tibetan cashmere goats skin tissues.…”

Section: Discussionmentioning

confidence: 92%

Supplemental Information 3: The top 10 differentially expressed lncRNAs with target genes in Tibetan cashmere goats

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Upregulation of PUM1 Expression in Preeclampsia Impairs Trophoblast Invasion by Negatively Regulating the Expression of the lncRNA HOTAIR

Cited by 40 publications

References 41 publications

Tissue‐resident CD8⁺T memory cells with unique properties are present in human decidua during early pregnancy

Tissue‐resident CD8⁺T memory cells with unique properties are present in human decidua during early pregnancy

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

Supplemental Information 3: The top 10 differentially expressed lncRNAs with target genes in Tibetan cashmere goats

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Upregulation of PUM1 Expression in Preeclampsia Impairs Trophoblast Invasion by Negatively Regulating the Expression of the lncRNA HOTAIR

Cited by 40 publications

References 41 publications

Tissue‐resident CD8+T memory cells with unique properties are present in human decidua during early pregnancy

Tissue‐resident CD8+T memory cells with unique properties are present in human decidua during early pregnancy

High glucose condition inhibits trophoblast proliferation, migration and invasion by downregulating placental growth factor expression

Supplemental Information 3: The top 10 differentially expressed lncRNAs with target genes in Tibetan cashmere goats

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Tissue‐resident CD8⁺T memory cells with unique properties are present in human decidua during early pregnancy

Tissue‐resident CD8⁺T memory cells with unique properties are present in human decidua during early pregnancy