Upregulation of RAB7 Relates To Neuronal Pyroptosis After Spinal Cord Injury In Rat

Abstract

The Rab7 belongs to the Ras small GTPase superfamily, and the abnormal expression of Rab7 can cause neuropathy and lipid metabolism disease. However, the regulatory mechanism was not explicitly clear. Studies show that its plays crucial role inner membrane translocase. However, the role of Rab7 on the regulatory mechanisms of cell survival is still unknown. We performed a rat spinal cord injury (SCI) model to explore the cellular localization and expression of Rab7 after SCI in this study. Western blot has found that Rab7 was expressed in the spinal cord tissue. On the first day, it significantly increased, then decreased at 48 h, and on the third day reached a peak after SCI. what important is that Rab7 was colocalized with neurons. Furthermore, Western blot was also demonstrated that the pyroptosis-related protein (GSDMD , Caspase-1, ASC) expression attained the peak after the third-day post-injury. Importantly, the immunohistochemistry analysis revealed that Rab7 was completely colocalized with ASC in the neuron after SCI. These results suggested that Rab7 was colocalized with the neurons and involved in the pyroptosis of neurons and closely related to the spinal cord after injury.