Upregulation of the Elongation Factor-1α Gene by p53 in Association With Death of an Erythroleukemic Cell Line

Kato, Mitsuo; Sato, Hiromi; Nagayoshi, Mariko; Ikawa, Yoji

doi:10.1182/blood.v90.4.1373.1373_1373_1378

Cited by 13 publications

(15 citation statements)

References 36 publications

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“…For example, from a subset of the same Chinese collection, 51 nucleotide haplotypes were identified in the 96 Avr3a sequences (Yang et al, ). The eEF‐1α is a housekeeping gene playing multifaceted roles in the biochemical and physiological processes of life (Chang et al, ; Kato, Sato, Nagayoshi, & Ikawa, ). Low genetic variation in eEF‐1α is consistent with evolutionary hypothesis that genes important to cell functions evolve at a reduced rate (Jordan, Rogozin, Wolf, & Koonin, ).…”

Section: Discussionmentioning

confidence: 99%

Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production

Wang

Xie

et al. 2019

Evolutionary Applications

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Gene flow is an important evolutionary force that enables adaptive responses of plant pathogens in response to changes in the environment and plant disease management strategies. In this study, we made a direct inference concerning gene flow in the Irish famine pathogen Phytophthora infestans between two of its hosts (potato and tomato) as well as between China and India. This was done by comparing sequence characteristics of the eukaryotic translation elongation factor 1 alpha (eEF-1α) gene, generated from 245 P. infestans isolates sampled from two countries and hosts. Consistent with previous results, we found that eEF-1α gene was highly conserved and point mutation was the only mechanism generating any sequence variation. Higher genetic variation was found in the eEF-1α sequences in the P. infestans populations sampled from tomato compared to those sampled from potato. We also found the P. infestans population from India displayed a higher genetic variation in the eEF-1α sequences compared to China. No gene flow was detected between the pathogen populations from the two countries, which is possibly attributed to the geographic barrier caused by Himalaya Plateau and the minimum cross-border trade of potato and tomato products. The implications of these results for a sustainable management of late blight diseases are discussed. K E Y W O R D Selongation factor-1α (eEF-1α), haplotype network, phylogenetic analysis, Phytophthora infestans, population connectivity, population genetic structure | 319 WANG et Al.

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Section: Discussionmentioning

confidence: 99%

Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production

Wang

Xie

et al. 2019

Evolutionary Applications

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“…Such functions include actin filament bundling (Yang et al, 1990), oncogenic transformation (Tatsuka et al, 1992), microtubule severing (Shiina et al, 1994), ubiquitin-dependent proteolysis of N terminus-blocked proteins (Gonen et al, 1994), and activation of serum starvation-induced or p53-induced apoptosis with its upregulation or overexpression (Kato et al, 1997;Duttaroy et al, 1998). These functions were demonstrated by using cells of non-neuronal origin, such as erythroleukemia cells (Kato et al, 1997) and mouse 3T3 fibroblasts (Duttaroy et al, 1998). In all of these cases the increase of the intracellular level of EF-1␣ occurred in parallel with the induction of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Purification of Polyglutamine Aggregates and Identification of Elongation Factor-1α and Heat Shock Protein 84 as Aggregate-Interacting Proteins

Mitsui¹,

Nakayama

Akagi³

et al. 2002

J. Neurosci.

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Aggregates of green fluorescent protein (GFP)-fused truncated N-terminal huntingtin containing abnormally long polyglutamine tracts (150 repeats of glutamine residue) were purified from an ecdysone-inducible mutant neuro2A cell line (HD150Q-28) by using a fluorescence-activated cell sorter. To analyze the aggregate-interacting proteins, we subjected the purified aggregates to SDS-PAGE; prominent protein bands in the gel were digested with Achromobactor lysyl endopeptidase, followed by a HPLC-mass spectrometry (MS) analysis. The resulting data of tandem MS analysis revealed that, in addition to ubiquitin and widely reported chaperone proteins such as heat shock cognate 70 (HSC70), human DNA J-1 (HDJ-1), and HDJ-2, the translational elongation factor-1alpha (EF-1alpha) and heat shock protein 84 (HSP84) also were recognized as aggregate-interacting proteins. Sequestration of these proteins to aggregates was confirmed further by several immunochemical methods. We confirmed that, in addition to the other known proteins, EF-1alpha and HSP84 also colocalized with the intracellular aggregates. An assay of the transient expression of EF-1alpha and HSP84 in HD150Q-28 cells revealed that both proteins improved cell viability. Moreover, the rate of aggregate formation decreased in both transfectants. Our study suggests that both EF-1alpha and HSP84 are involved in the neurodegenerative process of polyglutamine diseases.

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“…The control of actin organization by EF1␣ may be linked to cell-cycle progression. In higher eukaryotes, it has been demonstrated that the overproduction of EF1␣ is related to oncogenic transformation (Tatsuka et al 1992;Kato et al 1997), metastasis (Taniguchi et al 1992;Pencil et al 1993;Edmonds et al 1996) and delayed cell senescence (Shepherd et al 1989). These results indicate that the regulation of multifunctional EF1␣ is important for normal cell proliferation and differentiation.…”

Section: Figurementioning

confidence: 98%

Overproduction of elongation factor 1α, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast

et al. 1999

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Background: Elongation factor 1␣ (EF1␣), an essential component of the eukaryotic translational machinery, has been shown to possess various biochemical and biological activities, including F-actin-binding and -bundling, microtubulesevering, and the activity of making fibroblasts highly susceptible to transformation. However, our understanding of the biological significance of EF1␣ with respect to these various biochemical or biological activities remains limited. Here we report the identification of EF1␣-encoding genes as genes whose over-expression causes aberrant cell morphology in fission yeast.

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Upregulation of the Elongation Factor-1α Gene by p53 in Association With Death of an Erythroleukemic Cell Line

Cited by 13 publications

References 36 publications

Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production

Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production

Purification of Polyglutamine Aggregates and Identification of Elongation Factor-1α and Heat Shock Protein 84 as Aggregate-Interacting Proteins

Overproduction of elongation factor 1α, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast

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