Upregulation of TNF-α and IL-3 expression in lesional and uninvolved skin in different types of urticaria

Hermes, Bárbara; Prochazka, Ann-Karin; Haas, Norbert; Jurgovsky, K; Sticherling, Michael; Henz, Beate M.

doi:10.1016/s0091-6749(99)70506-3

Cited by 171 publications

(133 citation statements)

References 45 publications

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“…36,37 Before exposure to antibodies, antigen unmasking was performed by incubating the skin sections in a commercial antigen retrieval buffer (pH 9.9) (Target Retrieval Solution; DakoCytomation, Hamburg, Germany) for 30 minutes at 95°C. Immunoreactivity for bcl-2 and bcl-xL was then investigated using a mouse anti-human bcl-2 mAb (clone 100/ D5; dilution 1:100; Zymed Laboratories, South San Francisco, CA), a mouse anti-human bcl-xL mAb (clone 7B2.5; dilution 1:500; DPC Biermann, Bad Nauheim, Germany), and the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Immunoreactivity for bcl-2 and bcl-xL was then investigated using a mouse anti-human bcl-2 mAb (clone 100/ D5; dilution 1:100; Zymed Laboratories, South San Francisco, CA), a mouse anti-human bcl-xL mAb (clone 7B2.5; dilution 1:500; DPC Biermann, Bad Nauheim, Germany), and the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. 37 Optimal dilutions of the antibodies were tested with appropriate positive and negative control specimens such as tonsils or tumors. Evaluation of skin sections was performed by recording the distribution of positive cells and by counting the numbers of toluidine blue-positive and immunoreactive mast cells in at least five microscopic fields at high-power magnification (ϫ400).…”

Section: Immunohistochemistrymentioning

confidence: 99%

See 1 more Smart Citation

Expression of Bcl-2 and Bcl-xL in Cutaneous and Bone Marrow Lesions of Mastocytosis

Hartmann

Artuc

Baldus

et al. 2003

The American Journal of Pathology

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Mastocytosis is a rare disease characterized by accumulation of mast cells in tissues.To investigate whether an altered regulation of mast cell apoptosis might be involved in the pathogenesis of mastocytosis, expression of the apoptosis-preventing molecules bcl-2 and bcl-xL was studied by immunohistochemistry in skin and bone marrow lesions of mastocytosis patients. In addition, reverse transcription-polymerase chain reaction was used to investigate levels of bcl-2 and bcl-xL mRNA in cutaneous mastocytosis lesions. Since activating mutations of c-kit are known to be associated with some forms of mastocytosis, human mast cell cultures were also stimulated via c-kit and the expression of bcl-2 and bcl-xL was assessed by immunoblotting. In patients with mastocytosis, the expression of bcl-2 protein but not bcl-xL in cutaneous mast cells was significantly enhanced, compared to healthy controls. Evaluating different subgroups of adult and pediatric mastocytosis patients, all groups were found to express significantly increased levels of bcl-2 protein, and none of the patient groups was found to overexpress bcl-xL, with the exception of solitary mastocytomas that showed a tendency for up-regulated bcl-xL protein. Furthermore, the expression of bcl-2 mRNA was significantly enhanced in cutaneous lesions of adult and pediatric patients, while bcl-xL mRNA levels were only slightly increased in pediatric, but not in adult patients with mastocytosis. In contrast to the skin lesions, bone marrow infiltrates of patients with systemic mastocy- Mastocytosis represents a heterogeneous group of diseases characterized by an increase of mast cells in different organs.

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Section: Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Expression of Bcl-2 and Bcl-xL in Cutaneous and Bone Marrow Lesions of Mastocytosis

Hartmann

Artuc

Baldus

et al. 2003

The American Journal of Pathology

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“…16,17 In another trial related to the skin biopsies of patients with FM, Salemi et al 18 showed that a high proportion of the patients had increased immunoreactivity for tumor necrosis factor alpha (TNF-a) in the biopsy specimens. Similarly, Hermes et al 19 demonstrated that there was a high immunoreactivity for TNF-a in all types of urticaria, whereas none of the control patients had an immunohistochemical expression of TNF-a in the biopsy specimens.…”

Section: Discussionmentioning

confidence: 79%

“…13,14 Some pathological changes have been described in the skin biopsies of patients with FM as resembling the cellular and cytokine involvement in the biopsy specimens of patients with CU. [15][16][17][18][19] In addition, it has been suggested that there is an autonomic dysfunction in patients with FM and CU, and sympathomimetic amines may lead to improvement in the symptoms of the two diseases when there is a resistance to conventional therapies. [20][21][22][23] Taking into account all of this information, we investigated whether patients with CU are also affected by FM.…”

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confidence: 99%

Is There An Association Between Chronic Urticaria and Fibromyalgia Syndrome?

et al. 2014

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Objectives: Chronic urticaria (CU) and fibromyalgia (FM) are different types of diseases with unclear etiopathogeneses but share many clinical and histochemical features. This study aims to make a recognization on these features and examines whether patients with CU are also affected by FM. Patients and methods:Forty patients with CU and 38 healthy controls were enrolled to this study. All of the participants were assessed according to the 1990 American College of Rheumatology (ACR) classification criteria for FM and asked questions regarding the clinical features of FM. The quality of life was assessed by the Nottingham Health Profile (NHP), while the psychological status was evaluated using the Hospital Anxiety and Depression Scale (HADS). Results:The incidence of FM was higher among the patients with CU (32.5%) than the controls (10.5%) (p=0.019). We indicated that the patients with CU suffered restrictions regarding to quality of life as assessed by the NHP. NHP-sleep (p=0.035), NHP-social isolation (p=0.032) and NHP-emotional reaction (p=0.027) scores were significantly higher compared to HCs. The HADS-depression scores were also significantly higher in the patients with CU (p=0.006). The patients with CU and concomitant FM had higher total NHP scores than those without FM (p<0.001). Conclusion:Clinicians must be alerted to the possible coexistence of FM in CU patients. Patients with CU have higher rates of FM than the general population and this results in more restrictions in daily life in these patients than those with CU alone. Therefore, additional treatment protocols may be required to be implicated for the treatment of patients with both CU and FM.

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“…En la urticaria adquirida se han encontrado niveles elevados de histamina, del factor de necrosis tumoral alfa (TNF-α), de leucotrienos, de prostaglandina D2 y del factor activador de plaquetas (PAF) (37). En un estudio de nueve pacientes se encontraron autoanticuerpos contra la inmunoglobulina E (IgE) o contra el receptor de alta afinidad de la IgE (FcεRI), y se postuló que podrían estar involucrados mecanismos autoinmunitarios debido a que, al transferir suero de pacientes a individuos sanos por inyección subcutánea, los sujetos sanos desarrollaron habones (34).…”

Section: Urticaria Por Contacto Con El Fríounclassified

Urticaria inducible: serie de casos y revisión de la literatura

Amaya

Sánchez

2015

biomedica

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Contribución de los autores:Jorge Sánchez: idea central del artículo Todos los autores contribuyeron por igual en la redacción y revisión del manuscrito. Las urticarias inducibles constituyen un grupo heterogéneo de trastornos cutáneos caracterizados por la aparición de habones, prurito o angioedema, que en ocasiones se acompañan de síntomas sistémicos causados por estímulos inocuos para la mayoría de la población, como el frío, el calor, la presión, etc., y que comprometen la calidad de vida de los pacientes. La mayor parte de la literatura médica pertinente proviene de reportes y series de casos, ya que su epidemiología se ha estudiado poco. El objetivo de esta revisión es ofrecer una visión actualizada de la información disponible sobre varios tipos de urticaria inducida, mediante la presentación de un caso clínico ilustrativo y la descripción de los mecanismos fisiopatológicos, las manifestaciones clínicas y el tratamiento de cada condición. Urticaria inducible: serie de casos y revisión de la literaturaPalabras clave: alergia e inmunología, angioedema, enfermedades de la piel, urticaria, edema doi: http://dx.doi.org/10.7705/biomedica.v36i1.2678 Inducible urticaria: Case series and literature reviewInducible urticaria is a heterogeneous group of skin disorders characterized by the appearance of wheals, pruritus and/or angioedema, sometimes accompanied by systemic symptoms caused by innocuous stimuli (cold, heat, pressure, etc.). This group of disorders compromises people's quality of life and most of the literature in this regard comes from case reports and case series since its epidemiology has been poorly studied and some cases are very rare. The aim of this review is to show an up-to-date overview of the available literature for various types of inducible urticarias, always beginning with an illustrative case and then describing their pathophysiological mechanisms, clinical manifestations, and treatment. La urticaria es una enfermedad cutánea y de las mucosas caracterizada por la aparición de habones o angioedema que es preciso diferenciar de otras condiciones médicas en las que pueden presentarse habones, angioedema o los dos, por ejemplo, la anafilaxia, los síndromes autoinflamatorios o el angioedema hereditario (1). Según su duración se ha clasificado en aguda y crónica, definiéndose esta como aquella con una duración mayor a seis semanas. La prevalencia de la urticaria crónica se estima entre 0,5 y 5 % de la población general. Según algunos estudios, en la población estadounidense la incidencia es de 1,4 % por año, aproximadamente (2). La European Academy of Allergy and ClinicalImmunology (EAACI) ha propuesto recientemente una clasificación etiológica más acorde con el conocimiento actual de los mecanismos subyacentes de la enfermedad, que incluye bajo el nombre de "urticaria inducible" aquellas desencadenadas por estímulos físicos comúnmente inocuos como, por ejemplo, el calor, el frío, la presión aplicada a la piel, el ejercicio, la vibración, la luz solar y los

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Upregulation of TNF-α and IL-3 expression in lesional and uninvolved skin in different types of urticaria

Cited by 171 publications

References 45 publications

Expression of Bcl-2 and Bcl-xL in Cutaneous and Bone Marrow Lesions of Mastocytosis

Expression of Bcl-2 and Bcl-xL in Cutaneous and Bone Marrow Lesions of Mastocytosis

Is There An Association Between Chronic Urticaria and Fibromyalgia Syndrome?

Urticaria inducible: serie de casos y revisión de la literatura

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