2015
DOI: 10.3791/52636
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Upright Imaging of <em>Drosophila</em> Egg Chambers

Abstract: Drosophila melanogaster oogenesis provides an ideal context for studying varied developmental processes since the ovary is relatively simple in architecture, is well-characterized, and is amenable to genetic analysis. Each egg chamber consists of germ-line cells surrounded by a single epithelial layer of somatic follicle cells. Subsets of follicle cells undergo differentiation during specific stages to become several different cell types. Standard techniques primarily allow for a lateral view of egg chambers, … Show more

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Cited by 1 publication
(4 citation statements)
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“…To observe the pattern of border cell specification at the anterior, we developed a method to observe egg chambers end-on 24 . We first used the genotype slbo-Gal4, UAS-mCD8-GFP (referred to here as the slbo reporter 25 , 26 , Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…To observe the pattern of border cell specification at the anterior, we developed a method to observe egg chambers end-on 24 . We first used the genotype slbo-Gal4, UAS-mCD8-GFP (referred to here as the slbo reporter 25 , 26 , Fig.…”
Section: Resultsmentioning
confidence: 99%
“…1a ) to mark the border cells, which reveals the Slbo expression gradient across the anterior epithelium. We manually sorted and mounted individual egg chambers in glycerin jelly standing with the anterior end facing up 24 , 27 ( Fig. 1b ).…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations