Uptake and biological effects of chitosan-capped gold nanoparticles on Chinese Hamster Ovary cells

Ovarian dysfunction and gene-expressed characteristics of female mice caused by long-term exposure to titanium dioxide nanoparticles

“…Recently, the toxicologic characteristics of some nanomaterials in ovarian cells in vitro have been reported. For instance, gold nanoparticles were shown to enter rat ovarian granulosa cells and subcellular organelles and alter estrogen accumulation in vitro [23], and were able to traverse the cell membrane and enter Chinese hamster ovary cells by the endocytic pathway [24]. In addition, calcium phosphate nanoparticles entered granulosa cells, and were distributed in the membranous compartments, including lysosome, mitochondria and intracellular vesicles, affected hormone production, and caused apoptosis in human granulosa cells [25].…”

Designing Gold Nanoparticle-Ensembles as Surface Enhanced Raman Scattering Tags inside Human Retinal Cells

“…The particles were fabricated by an alternative method of the commonly Turkevich-Frens synthesis, by the use of ascorbic acid as reducer of the gold salt [18]. Notably, this class of nanoparticles does not require any supplementary operation of encoding since ascorbic acid remains attached in between nanoparticles and give a persistent and strong SERS signal when nano-tags are internalized by cells.…”

“…All glassware used in the synthesis was cleaned in a bath of freshly prepared aqua regia solution (3 : 1 (v/v) HNO 3 : HCl) and rinsed thoroughly prior to use. The colloidal gold nanoparticles were synthesized based on a method described elsewhere in detail [18]. Briefly, individual nanoparticles were produced by stirring 10 mL of a solution of 0.5·10 −3 M tetrachloroauric acid (HAuCl 4 ) at room temperature for several minutes, followed by the addition of a proper volume of a freshly prepared ascorbic acid (7.5·10 −3 M) solution.…”

Preparation and characterization of chitosan-capped radioactive gold nanoparticles: neutron irradiation impact on structural properties

“…Chitosan is a copolymer of glucosamine and N-acetylglucosamine with primary amine groups available for interactions. It has been investigated extensively as a potential drug carrier, due to its biocompatible, versatile, inexpensive and non-toxic properties [17,18]. Recent studies show that chitosan exhibits anticancer properties both in vitro and in animal models [19,20].…”