Uptake and Cytotoxicity of Docetaxel-Loaded Hyaluronic Acid-Grafted Oily Core Nanocapsules in MDA-MB 231 Cancer Cells

Youm, Ibrahima; Agrahari, Vivek; Murowchick, James B.; Youan, Bi‐Botti C.

doi:10.1007/s11095-014-1339-x

Cited by 43 publications

(25 citation statements)

References 35 publications

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“…In the case of DTX-LPH, enhanced cellular DTX retention, intracellular acid-responsive drug release, and minimization of drug loss due to efflux resulted in enhanced cytotoxicity. 28 Blank LPH did not cause any significant cytotoxicity in any cell lines across all concentrations tested (up to 50 µg/mL).…”

Section: In Vitro Cell Studiesmentioning

confidence: 84%

Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells

Kim¹,

Tran²,

Ramasamy³

et al. 2015

IJN

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The attachment of polyethylene glycol (PEG) increases the circulation time of drug-containing nanoparticles; however, this also negatively affects cellular uptake. To overcome this problem, unique lipid polymer hybrid (LPH) nanoparticles were developed with a pH-responsive PEG layer that detached prior to cell uptake. Docetaxel (DTX) was incorporated into the lipid core of the nanoparticles, which was then shielded with the pH-responsive block co-polymer polyethylene glycol- b -polyaspartic acid (PEG- b -PAsp) using a modified emulsion method. The optimized LPH nanoparticles were ~200 nm and had a narrow size distribution. Drug release from DTX-loaded LPH (DTX-LPH) nanoparticles was pH-sensitive, which is beneficial for tumor targeting. More importantly, DTX-LPH nanoparticles were able to effectively induce apoptosis in cancer cells. The negative surface charge and PEG shell of vehicle remarkably enhanced the blood circulation and physiological activity of DTX-LPH nanoparticles compared with that of free DTX. The nanoparticles were also found to reduce the size of tumors in tumor-bearing xenograft mice. The in vivo anticancer effect of DTX-LPH nanoparticles was further confirmed by the elevated levels of caspase-3 and poly ADP ribose polymerase found in the tumors after treatment. Thus, the results suggest that this novel LPH system could be an effective new treatment for cancer.

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Section: In Vitro Cell Studiesmentioning

confidence: 84%

Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells

Kim¹,

Tran²,

Ramasamy³

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…• Avoiding off-target effect Design of experiment approach is also a valuable tool for preformulation development and can be employed to optimize the formulation parameters for a small molecule and macromolecule drugs as several factors can be screened to analyze their individual/interactive effects in formulation development as used by several researchers [45,[103][104][105]. Traditional approaches for formulation development involve the time consuming process of varying one factor at a time and examining its effect, which requires a large number of experimental runs.…”

Section: Future Science Groupmentioning

confidence: 99%

Nanocarrier Fabrication and Macromolecule Drug delivery: Challenges and Opportunities

2016

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Macromolecules (proteins/peptides) have the potential for the development of new therapeutics. Due to their specific mechanism of action, macromolecules can be administered at relatively low doses compared with small-molecule drugs. Unfortunately, the therapeutic potential and clinical application of macromolecules is hampered by various obstacles including their large size, short in vivo half-life, phagocytic clearance, poor membrane permeability and structural instability. These challenges have encouraged researchers to develop novel strategies for effective delivery of macromolecules. In this review, various routes of macromolecule administration (invasive/noninvasive) are discussed. The advantages/limitations of novel delivery systems and the potential role of nanotechnology for the delivery of macromolecules are elaborated. In addition, fabrication approaches to make nanoformulations in different shapes and sizes are also summarized.

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“…Ligand/receptor targeting has proven to be an effective method for drug delivery [9]. In order to improve efficacy chemotherapeutic agents need to be delivered into tumor cell cytoplasm or sub-cellular organelles such as nucleus and mitochondria.…”

Section: Novel Strategies For Cancer Targeted Deliverymentioning

confidence: 99%

Novel delivery approaches for cancer therapeutics

Mitra

Agrahari

Mandal

et al. 2015

Journal of Controlled Release

138

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Currently, a majority of cancer treatment strategies are based on the removal of tumor mass mainly by surgery. Chemical and physical treatments such as chemo- and radiotherapies have also made a major contribution in inhibiting rapid growth of malignant cells. Furthermore, these approaches are often combined to enhance therapeutic indices. It is widely known that surgery, chemo- and radiotherapy also inhibit normal cells growth. In addition, these treatment modalities are associated with severe side effects and high toxicity which in turn lead to low quality of life. This review encompasses novel strategies for more effective chemotherapeutic delivery aiming to generate better prognosis. Currently, cancer treatment is a highly dynamic field and significant advances are being made in the development of novel cancer treatment strategies. In contrast to conventional cancer therapeutics, novel approaches such as ligand or receptor based targeting, triggered release, intracellular drug targeting, gene delivery, cancer stem cell therapy, magnetic drug targeting and ultrasound-mediated drug delivery, have added new modalities for cancer treatment. These approaches have led to selective detection of malignant cells leading to their eradication with minimal side effects. Lowering, multi-drug resistance and involving influx transportation in targeted drug delivery to cancer cells can also contribute significantly in the therapeutic interventions in cancer.

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Uptake and Cytotoxicity of Docetaxel-Loaded Hyaluronic Acid-Grafted Oily Core Nanocapsules in MDA-MB 231 Cancer Cells

Abstract: The use of HA-grafted NCs enhanced the selective drug payload, cytotoxicity and uptake in MDA-MB 231 cells. Therefore, it could be a promising template for safe and effective delivery of Doc and similar chemotherapeutic agents in cancer cells.

Cited by 43 publications

References 35 publications

Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells

Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells

Nanocarrier Fabrication and Macromolecule Drug delivery: Challenges and Opportunities

Novel delivery approaches for cancer therapeutics

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