Uptake and detoxification of salicylic acid by Vicia faba and Fagopyrum esculentum

Schulz, Margot; Schnabl, Heide; Manthe, Barbara; Schweihofen, Barbara; Casser, Ingrid

doi:10.1016/0031-9422(93)85505-l

Cited by 31 publications

(27 citation statements)

References 14 publications

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“…(Lee et al 1995). Studies of SA metabolism in Mallotus japonicas, Vicia faba, and Nicotiana tabacum showed that SAG is the predominant stable metabolite of SA while SA glucose ester (SGE) is a relatively minor metabolite (Lee and Raskin 1999;Schulz et al 1993;Tanaka et al 1990). Conversion of SA into SAG has been reported to be involved in systemic acquired resistance (SAR) in tobacco (Hennig et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Metabolic movement upon abscisic acid and salicylic acid combined treatments

Okamoto

Tsuboi

Chikayama

et al. 2009

Plant Biotechnology

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Section: Discussionmentioning

confidence: 99%

Metabolic movement upon abscisic acid and salicylic acid combined treatments

Okamoto

Tsuboi

Chikayama

et al. 2009

Plant Biotechnology

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“…The observation that SA uptake is more than directly proportional to concentration of SA (Fig. 3), and the active uptake of SA by bean roots (Schulz et al 1993), both indicate phloem loading. Therefore, we speculate that the applied SA is rapidly loaded in phloem cells, which are involved in the generation of the systemic signal for SAR (Smith-Becker et al 1998) and might have a high sensitivity to SA.…”

Section: Discussionmentioning

confidence: 99%

Nanogram Amounts of Salicylic Acid Produced by the RhizobacteriumPseudomonas aeruginosa7NSK2 Activate the Systemic Acquired Resistance Pathway in Bean

Meyer¹,

Capieau²,

Audenaert³

et al. 1999

MPMI

212

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Root colonization by specific nonpathogenic bacteria can induce a systemic resistance in plants to pathogen infections. In bean, this kind of systemic resistance can be induced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 and depends on the production of salicylic acid by this strain. In a model with plants grown in perlite we demonstrated that Pseudomonas aeruginosa 7NSK2-induced resistance is equivalent to the inclusion of 1 nM salicylic acid in the nutrient solution and used the latter treatment to analyze the molecular basis of this phenomenon. Hydroponic feeding of 1 nM salicylic acid solutions induced phenylalanine ammonia-lyase activity in roots and increased free salicylic acid levels in leaves. Because pathogen-induced systemic acquired resistance involves similar changes it was concluded that 7NSK2-induced resistance is mediated by the systemic acquired resistance pathway. This conclusion was validated by analysis of phenylalanine ammonia-lyase activity in roots and of salicylic acid levels in leaves of soil-grown plants treated with Pseudomonas aeruginosa. The induction of systemic acquired resistance by nanogram amounts of salicylic acid is discussed with respect to long-distance signaling in systemic acquired resistance.

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“…These data suggest the free SA as the active form responsible for SA function in cells. In roots of Vicia faba and Fagopyrum esculentum, exogenous SA was conjugated into O-glucoside as a detoxi®cation mechanism (Schulz et al, 1993). Therefore, a possible role of conjugated SA as a detoxi®cation mechanism is suggested in tobacco suspension-cultured cells.…”

Section: Discussionmentioning

confidence: 99%

Ca 2+ ‐dependent and Ca 2+ ‐independent excretion modes of salicylic acid in tobacco cell suspension culture

Chen¹,

Hou²,

Kúc³

et al. 2001

Journal of Experimental Botany

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14C-salicylic acid (SA) was used to monitor SA metabolism and its regulation in tobacco cell suspension culture. Two SA concentrations (20 mM and 200 mM) were used for comparison. SA was quickly taken up in both treatments, and the 200 mM-treated cells absorbed approximately 15 times that of 20 mMtreated cells within 5 min. More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 mM and 20 mM SA, respectively. SA excretion was significantly inhibited by EGTA and the inhibition could be reversed by the addition of exogenous Ca 2q to the culture medium in the 200 mM SA treatment. However, EGTA had little or no effect on SA excretion in the 20 mM SA treatment. The data suggest that tobacco suspension-cultured cells may contain both Ca 2q -dependent and Ca 2q -independent pathways for SA excretion. Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 mM but not in the 20 mM SA treatment. These data support the existence of alternative SA excretion pathways in tobacco suspension-cultured cells. Tobacco cells may use both Ca 2q -dependent and Ca 2q -independent excretion pathways to cope with different intracellular SA status, and the pathway influenced by EGTA, reduced glutathione, staurosporine, and cycloheximide is activated by SA at 200 mM, but not at 20 mM.

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Uptake and detoxification of salicylic acid by Vicia faba and Fagopyrum esculentum

Cited by 31 publications

References 14 publications

Metabolic movement upon abscisic acid and salicylic acid combined treatments

Metabolic movement upon abscisic acid and salicylic acid combined treatments

Nanogram Amounts of Salicylic Acid Produced by the RhizobacteriumPseudomonas aeruginosa7NSK2 Activate the Systemic Acquired Resistance Pathway in Bean

Ca 2+ ‐dependent and Ca 2+ ‐independent excretion modes of salicylic acid in tobacco cell suspension culture

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