Uptake and Distribution of Nitrogen in Perennial Ryegrass: Effect of Additional Applications at Vegetative Growth

Gislum, René; Wollenweber, Bernd; Boelt, Birte; Jensen, Erik Steen

doi:10.1081/pln-120025466

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…A widely adopted application rate recommendation was 20-60 Urea-N kg ha -1 after grazing (Rawnsley et al, 2019). Sometimes the rates are greater in more intensive systems (Gislum et al, 2003;Gourley et al, 2017). Differences in NUE among perennial ryegrass cultivars after defoliation were reported using hydroponics (Wilkins et al, 1997) and field trials under variable N application rates (Wilkins et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Rapid Screening of Nitrogen Use Efficiency in Perennial Ryegrass (Lolium perenne L.) Using Automated Image-Based Phenotyping

et al. 2020

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Perennial ryegrass (Lolium perenne L.) is a dominant species in temperate Australian pastures. Currently, nitrogenous fertilizers are used to support herbage production for pasture and fodder. Increasing the nitrogen use efficiency (NUE) of pasture grasses could decrease the amount of fertilizer application and reduce nitrogen (N) leaching into the environment. NUE, defined as units of dry matter production per unit of supplied nitrogen, is a complex trait in which genomic selection may provide a promising strategy in breeding. Our objective was to develop a rapid, high-throughput screening method to enable genomic selection for y-60NUE in perennial ryegrass. NUE of 76 genotypes of perennial ryegrass from a breeding population were screened in a greenhouse using an automated imagebased phenomics platform under low (0.5 mM) and moderate (5 mM) N levels over 3 consecutive harvests. Significant (p < 0.05) genotype, treatment, and genotype by treatment interactions for dry matter yield and NUE were observed. NUE under low and moderate N treatments was significantly correlated. Of the seven plant architecture features directly extracted from image analysis and four secondarily derived measures, mean projected plant area (MPPA) from the two side view images had the highest correlation with dry matter yield (r = 0.94). Automated digital image-based phenotyping enables temporal plant growth responses to N to be measured efficiently and non-destructively. The method developed in this study would be suitable for screening large populations of perennial ryegrass growth in response to N for genomic selection purposes.

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Section: Introductionmentioning

confidence: 99%

Rapid Screening of Nitrogen Use Efficiency in Perennial Ryegrass (Lolium perenne L.) Using Automated Image-Based Phenotyping

et al. 2020

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“…Traditionally whole plant shoots are sampled for forage nutritional diagnosis, however, attempts have been made to collect and analyze parts of the forage plant tops (Batista & Monteiro, 2007). Additional N applied to perennial ryegrass was primarily used to increase total-N content in leaves, which were the prefered N storage organs (Gislum et al, 2003). On the other hand, the newly expanded lamina leaf (NL) -formerly known as recently expanded lamina has been utilized as morphological component of plant tops, appropriate for the evaluation of the nutritional status, mainly for N and sulphur (Mattos & Monteiro, 2003;Batista & Monteiro, 2007;Lavres Jr. et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Nitrate reductase activity and spad readings in leaf tissues of guinea grass submitted to nitrogen and potassium rates

Lavres

Santos²,

Monteiro³

2010

Rev. Bras. Ciênc. Solo

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Nitrogen and K deficiency are among the most yield limiting factors in Brazilian pastures. The lack of these nutrients can hamper the chlorophyll biosynthesis and N content in plant tissues. A greenhouse experiment was carried out to evaluate the relationship among N and K concentrations, the indirect determination of chlorophyll content (SPAD readings), nitrate reductase activity (RNO3-) in newly expanded leaf lamina (NL) and the dry matter yield for plant tops of Mombaça grass (Panicum maximum Jacq.). A fractionated 5² factorial design was used, with 13 combinations of N and K rates in the nutrient solution. The experimental units were arranged in a randomized block design, with four replications. Plants were harvested twice. The first harvest occurred 36 days after seedling transplanting and the second 29 days after the first. Significance occurred for the interaction between the N and K rates to SPAD readings and to RNO3- assessment taken on the NL during the first growth. Besides, RNO3- and SPAD readings increased only with the NL N concentration, reaching the highest values of both variables up to about 25 g kg-1, but were ratively constant at higher leaf N. Significant relationships either between SPAD readings or RNO3- activity and shoot dry mass weight were also observed. The critical levels of N concentration in the NL were, respectively, 22 and 17g kg-1 in the first and second harvest. Thus, SPAD instrument and RNO3- assessment can be used as complementary tools to evaluate the N status in forage grass.

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Nitrogen and sulphur in Marandu grass: relationship between supply and concentration in leaf tissues

Batista

Monteiro²

2007

Sci. agric. (Piracicaba, Braz.)

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In order to better assess the nutritional status of forage grasses there has been an increasing interest in studying the nutrient concentrations present in different parts of plant shoot. The aim of this work was to evaluate the nitrogen and sulphur concentrations in leaf tissues of Brachiaria brizantha cv. Marandu grown in nutrient solutions with combined rates of both nitrogen and sulphur. An experiment was conducted in a greenhouse during the Spring season in Piracicaba-SP, Brazil, using ground quartz as substrate. A fractionated 5² factorial design was used, with 13 combinations of nitrogen and sulphur rates in the solutions, in mg L-1, as follows: 14 and 3.2; 14 and 32; 14 and 80; 126 and 12.8; 126 and 64; 210 and 3.2; 210 and 32; 210 and 80; 336 and 12.8; 336 and 64; 462 and 3.2; 462 and 32, and 462 and 80. The experimental units were set in a randomized block design, with four replications. Plants were harvested twice. Emerging leaves, recently expanded lamina and mature lamina were sampled separately at each harvest time. In these three types of tissues the nitrogen concentration was closely related to the nitrogen supply in the solution, whereas the sulphur concentration, N:S ratio and chlorophyll meter (SPAD) readings depended upon the supply of both nitrogen and sulphur. Mature lamina did not relate well to the changing in sulphur supply. To properly assess the nitrogen and sulphur concentrations, N:S ratio and SPAD readings for the Marandu grass, the two recently expanded lamina were the best tissues to indicate the availability of both nitrogen and sulphur in the nutrient solution.

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Uptake and Distribution of Nitrogen in Perennial Ryegrass: Effect of Additional Applications at Vegetative Growth

Cited by 3 publications

References 20 publications

Rapid Screening of Nitrogen Use Efficiency in Perennial Ryegrass (Lolium perenne L.) Using Automated Image-Based Phenotyping

Rapid Screening of Nitrogen Use Efficiency in Perennial Ryegrass (Lolium perenne L.) Using Automated Image-Based Phenotyping

Nitrate reductase activity and spad readings in leaf tissues of guinea grass submitted to nitrogen and potassium rates

Nitrogen and sulphur in Marandu grass: relationship between supply and concentration in leaf tissues

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