Uptake and effects of graphene oxide nanomaterials alone and in combination with polycyclic aromatic hydrocarbons in zebrafish

Martínez-Álvarez, Ignacio; Ménach, Karyn Le; Devier, Marie-Hélène; Barbarin, Iranzu; Cajaraville, Miren P.; Budzinski, Hélène; Orbea, Amaia

doi:10.1016/j.scitotenv.2021.145669

Cited by 25 publications

(17 citation statements)

References 76 publications

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“…Abnormalities/ clinical signs evidenced included alterations in locomotor activity, exploratory behaviour, and predator avoidance associated with a change in neurological behaviour in D. rerio exposed to GO (few layer, 3.0 μm) and rGO (few layer, 5.4 μm) at nominal concentrations of 0.1 and 1 mg/L and 0.1 and 0.5 mg/L, respectively (Audira et al, 2021). Effects in the brain including a reduction in acetylcholinesterase (AChE) activity and loss of dopaminergic neurons in the brains of D. rerio exposed to GO (few layer, 0.5 μm-<13 μm) at concentration of 2 mg/L were also reported (Martínez-Álvarez et al, 2021). This, together with gill and liver histopathological and biochemical alterations following exposure to other GO nanoforms, may indicate potential chronic effects that warrants further investigation and testing (Souza et al, 2017;Chen et al, 2016).…”

Section: Fish Acute Toxicitymentioning

confidence: 92%

“…Following a detailed review of the literature, 13 studies were identified relating to the toxicity testing of graphene materials using fish (Table 3). The materials tested included large sized (15 μm) graphene platelets (Al-Rudainy, 2019), both large (~500 nm) and small sized (~30 nm) FLG sheets (Lu et al, 2015), single layer GO (Souza et al, 2017(Souza et al, , 2019Jia et al, 2019;Sun et al, 2019;Martínez-Álvarez et al, 2021;Chen et al, 2016;Qiang et al, 2016), bi-layer rGO (Audira et al, 2021), as well as quantum dots (5-15 nm) modified through reduction, hydroxylation and amination (r-GQDs, OH-GQDs, NH 2 -GQD) (Hu et al, 2019). In 9 out of the 11 studies the fish species used was the zebrafish Danio rerio (2-8 months), while both a juvenile and adult common carp Cyprinus carpio were also used by Qiang et al (2016) and Al-Rudainy (2019), respectively.…”

Section: Fish Acute Toxicitymentioning

confidence: 99%

“…This can lead to uncertainties and a reduction in reliability of the data. In studies which monitored the exposure concentration (using UV-Vis spectroscopy) rapid settling and sedimentation of large sized GO was evidenced at concentrations >2 mg/L over time with complete loss of concentration over 72 h (Martínez-Álvarez et al, 2021;Chen et al, 2016). Authors have made reference to the observation of "megascopic precipitates" immediately after preparation of concentrations of 50 mg/L GO (Chen et al, 2016).…”

Section: Fish Acute Toxicitymentioning

confidence: 99%

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Applicability of OECD TG 201, 202, 203 for the aquatic toxicity testing and assessment of 2D Graphene material nanoforms to meet regulatory needs

et al. 2023

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Section: Fish Acute Toxicitymentioning

confidence: 92%

Section: Fish Acute Toxicitymentioning

confidence: 99%

Section: Fish Acute Toxicitymentioning

confidence: 99%

See 1 more Smart Citation

Applicability of OECD TG 201, 202, 203 for the aquatic toxicity testing and assessment of 2D Graphene material nanoforms to meet regulatory needs

et al. 2023

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“…This leaching can also occur during the adsorption process, making it difficult to control. Even though the toxic effects of GO are poorly understood, there is substantial evidence to show that GO has the ability to penetrate through the cell membranes of aquatic organisms [81,82]. Moreover, GO has the capacity to carry polycyclic aromatic hydrocarbons to aquatic organisms, causing significant toxicity [83].…”

Section: Adsorption At Graphene Oxidementioning

confidence: 99%

Graphene-Based Materials Immobilized within Chitosan: Applications as Adsorbents for the Removal of Aquatic Pollutants

et al. 2021

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Graphene and its derivatives, especially graphene oxide (GO), are attracting considerable interest in the fabrication of new adsorbents that have the potential to remove various pollutants that have escaped into the aquatic environment. Herein, the development of GO/chitosan (GO/CS) composites as adsorbent materials is described and reviewed. This combination is interesting as the addition of graphene to chitosan enhances its mechanical properties, while the chitosan hydrogel serves as an immobilization matrix for graphene. Following a brief description of both graphene and chitosan as independent adsorbent materials, the emerging GO/CS composites are introduced. The additional materials that have been added to the GO/CS composites, including magnetic iron oxides, chelating agents, cyclodextrins, additional adsorbents and polymeric blends, are then described and discussed. The performance of these materials in the removal of heavy metal ions, dyes and other organic molecules are discussed followed by the introduction of strategies employed in the regeneration of the GO/CS adsorbents. It is clear that, while some challenges exist, including cost, regeneration and selectivity in the adsorption process, the GO/CS composites are emerging as promising adsorbent materials.

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“…Samples were preserved at − 80 °C until analysis. We prioritised the use of males for the gene transcription levels based on the results obtained in previous studies [71,72,83].…”

Section: Gene Transcription Analysismentioning

confidence: 99%

Multilevel responses of adult zebrafish to crude and chemically dispersed oil exposure

Esteban‐Sánchez

Johann

Alemany³

et al. 2021

Environ Sci Eur

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Background The application of chemical dispersants is a common remediation strategy when accidental oil spills occur in aquatic environments. Breaking down the oil slick into small droplets, dispersants facilitate the increase of particulate and dissolved oil compounds, enhancing the bioavailability of toxic oil constituents. The aim of the present work was to explore the effects of water accommodated fractions (WAF) of a naphthenic North Sea crude oil produced with and without the addition of the chemical dispersant FINASOL OSR 52 to adult zebrafish exposed for 3 and 21 d. Fish were exposed to environmentally relevant concentrations of 5% and 25% WAFOIL (1:200) and to 5% WAFOIL+D (dispersant–oil ratio 1:10) in a semi-static exposure setup. Results The chemically dispersed WAF presented a 20-fold increase of target polycyclic aromatic hydrocarbons (PAHs) in the water phase compared to the corresponding treatment without dispersant and was the only treatment resulting in markedly bioaccumulation of PAHs in carcass after 21 d compared to the control. Furthermore, only 5% WAFOIL+D caused fish mortality. In general, the undispersed oil treatments did not lead to significant effects compared to control, while the dispersed oil induced significant alterations at gene transcription and enzyme activity levels. Significant up-regulation of biotransformation and oxidative stress response genes (cyp1a, gstp1, sod1 and gpx1a) was recorded in the livers. For the same group, a significant increment in EROD activity was detected in liver along with significant increased GST and CAT activities in gills. The addition of the chemical dispersant also reduced brain AChE activity and showed a potential genotoxic effect as indicated by the increased frequency of micronuclei in erythrocytes after 21 d of exposure. Conclusions The results demonstrate that the addition of chemical dispersants accentuates the effect of toxic compounds present in oil as it increases PAH bioavailability resulting in diverse alterations on different levels of biological organization in zebrafish. Furthermore, the study emphasizes the importance to combine multilevel endpoints for a reliable risk assessment due to high variable biomarker responses. The present results of dispersant impact on oil toxicity can support decision making for oil spill response strategies.

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Uptake and effects of graphene oxide nanomaterials alone and in combination with polycyclic aromatic hydrocarbons in zebrafish

Cited by 25 publications

References 76 publications

Applicability of OECD TG 201, 202, 203 for the aquatic toxicity testing and assessment of 2D Graphene material nanoforms to meet regulatory needs

Applicability of OECD TG 201, 202, 203 for the aquatic toxicity testing and assessment of 2D Graphene material nanoforms to meet regulatory needs

Graphene-Based Materials Immobilized within Chitosan: Applications as Adsorbents for the Removal of Aquatic Pollutants

Multilevel responses of adult zebrafish to crude and chemically dispersed oil exposure

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