Uptake and inactivation of thrombin by the fresh, glutardialdehyde or heparin treated human umbilical cord vein endothelium

Björck, C.-G.; Larsson, Rolf; Olsson, Per; Rothman, U.

doi:10.1016/0049-3848(81)90261-9

Cited by 17 publications

(4 citation statements)

References 9 publications

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“…Yet this function was recovered when they were treated with ethyl alcohol 50%. It has been suggested that the beneficial effect of the alcohol could be stabilization of the heparin layer possibly by precipitation of the 'structures' responsible for the heparin binding [2]; this nonetheless, remains to be proved.…”

Section: Discussionmentioning

confidence: 99%

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Decreased Acute Thrombogenicity of Human Umbilical Veins after Heparin and Alcohol Treatment

et al. 1983

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Nine glutaraldehyde-tanned human umbilical veins (GAUV) were incubated in heparin followed by storage in ethyl alcohol 50% (HAUV). These grafts were compared to five GAUV flushed with a heparinized solution without subsequent treatment in alcohol (HsAUV) and four nonheparinized GAUV (NHUV) placed in the contralateral carotid arteries of dogs for approximately 3 h. The thrombogenicity was evaluated by measuring the platelet adhesion and aggregation (⁵¹Cr), fibrinogen accumulation (¹²⁵I-fibrinogen), the thrombus-free surface and the thrombus weight. The HAUV had the lowest ⁵¹Cr radioactivity. The means of thrombus-free surface and thrombus weight for the grafts were as follows: HAUV, 82% and 0.3 g (n = 9); HsAUV, 53% and 0.6 g (n = 5); NHUV, 13% and 1.2 g (n = 4). The HAUV showed a lower thrombogenicity than both the NHUV and the HsAUV grafts.

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Section: Discussionmentioning

confidence: 99%

“…12]. The thrombogenicity of the graft mate rial is an important factor in early failures [3], It has recently been demonstrated [2] that fresh human umbilical veins inactivate thrombin but this function was lost when the i Supported by a gram from the Swedish Medical Research Council (B8I-17X-00759-15A).…”

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Decreased Acute Thrombogenicity of Human Umbilical Veins after Heparin and Alcohol Treatment

et al. 1983

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“…Blood velocity is a key factor in the ap parent thrombogenicity of a surface which, however, can be reduced [30,31]. Synthetic or chemically processed biological vascular prostheses have characteristic thrombotic threshold velocities below which thrombosis occurs [32], It is therefore important to match the diameter of the prosthesis to that of the adjacent blood vessel in order to maintain high blood velocities and avoid turbulence.…”

Section: Discussionmentioning

confidence: 99%

Chemically Fixed Human Umbilical Cord Vein Grafts as Arterial Substitutes: Potential and Limits

et al. 1986

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In spite of reported successes, synthetic fabric grafts and microporous and plain synthetic conduits have proven unsuitable for aorto-coronary bypasses and showed weaknesses below the knee. Readily available and uniform diameter vascular substitutes with biological and mechanical properties comparable to human vessels would be of paramount interest. Following reported successes with chemically fixed human umbilical veins (HUV), we have attempted to develop smaller diameter blood conduits and have improved the currently prevalent techniques of fixation, preparation and storage to generate more convenient surgical products. In vitro assessment of the processed HUV demonstrated that the HUV can be easily processed to make an arterial substitute that can be preserved either in a liquid medium or as a dry product. However, the in vivo implantations in dogs led to disappointing results for liquid-preserved or albuminated veins. Critical-point dried grafts gave better results, unfortunately they do not heal and they can only degrade after implantation.

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“…T h e thrombogenic reaction is complex, and this complexity has led to attempts to study isolated parts of the reaction, e.g. surface adsorption of plasma proteins (for review see Brash 1987), plasma coagulation (Bjorck et al 1981) and platelet activation (for review see Cooper et al 1976, Salzmann et al 1977. However, studies of isolated processes give limited information about Correspondence : Dr HHkan Nygren, Department of Histology, P.O.…”

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Adsorption of coagulation proteins and adhesion and activation of platelets at the blood‐solid interface. An experimental study of human whole blood

Nygren

Elam

Stenberg

1988

Acta Physiologica Scandinavica

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A procedure is presented allowing detailed studies of the adsorption of coagulation factors from whole blood on to surface. Anticoagulant (citrate or hirudin) was added to fresh venous blood. The blood was incubated in hydrophilic or hydrophobic glass tubes without contact with air. The adsorption of fibrinogen, fibronectin and factor IX was measured with an enzyme immunoassay using specific antibodies directed against these proteins. Adsorption of enzymically active kallikrein was measured using a chromogenic peptide substrate. Adhesion and activation of platelets was measured by direct examination in a scanning electron microscope and by measurement of release of beta-thromboglobulin. The results show that the adsorption of plasma proteins at the blood-solid interface is dependent on the anticoagulant used, surface energy of the test surface and incubation time. In experiments using hirudin a specific inactivator of thrombin, as anticoagulant, we found dynamic changes of the adsorbed protein film which could not be studied using citrated blood.

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Uptake and inactivation of thrombin by the fresh, glutardialdehyde or heparin treated human umbilical cord vein endothelium

Cited by 17 publications

References 9 publications

Decreased Acute Thrombogenicity of Human Umbilical Veins after Heparin and Alcohol Treatment

Decreased Acute Thrombogenicity of Human Umbilical Veins after Heparin and Alcohol Treatment

Chemically Fixed Human Umbilical Cord Vein Grafts as Arterial Substitutes: Potential and Limits

Adsorption of coagulation proteins and adhesion and activation of platelets at the blood‐solid interface. An experimental study of human whole blood

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