Uptake and incorporation ofmyo-inositol by bovine preimplantation embryos from two-cell to early blastocyst stages

Hynes, A. C.; Sreenan, J.M.; Kane, M. T.

doi:10.1002/(sici)1098-2795(200003)55:3<265::aid-mrd4>3.0.co;2-6

Cited by 18 publications

(13 citation statements)

References 29 publications

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“…3 H]myo-inositol was taken up by two-cell and four-cell embryos, morulae, and early blastocysts in a sodium-dependent manner, and was incorporated into PtdIns, PIP, and PIP2 (Hynes et al 2000). PIP3 was not detected in this analysis, but this may be due to the very transient nature of its synthesis under normal circumstances.…”

Section: Synthesis Of 3 0 -Phosphoinositidesmentioning

confidence: 72%

“…For a number of species, the addition of inositol to culture media improves embryo development (Kane 1988, 1989, Kane & Bavister 1988, Fahy & Kane 1994, Hynes et al 2000, suggesting that de novo synthesis of myo-inositol may be limiting during this stage of development (at least in vitro). Addition of inositol to culture media resulted in a 9.9-fold increase in thymidine incorporation into DNA and a 3.6-fold increase in amino acid incorporation into protein for rabbit morulae cultured through to expanded blastocysts (Fahy & Kane 1992).…”

Section: Synthesis Of 3 0 -Phosphoinositidesmentioning

confidence: 99%

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Phosphatidylinositol 3-kinase signaling in mammalian preimplantation embryo development

O’Neill

2008

Reproduction

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The development of the preimplantation mammalian embryo is an autopoietic process; once initiated development proceeds without an absolute requirement for external information or growth cues. This developmental autonomy is partly explained by the generation of autocrine trophic ligands that are released and act back on the embryo via specific receptors. Several embryotrophic ligands cause receptor-dependent activation of 1-o-phosphatidylinositol 3-kinase. This enzyme phosphorylates phosphatidylinositol-4,5-bisphosphate to form phosphatidylinositol-3,4,5-trisphosphate. Genetic or pharmacological ablation of this enzyme activity disrupts normal development of preimplantation embryos. Phosphatidylinositol-3,4,5-trisphosphate is a membrane lipid that acts as a docking site for a wide range of proteins possessing the pleckstrin homology (PH) domain. Such proteins are important regulators of cell survival, proliferation, and differentiation. RAC-a serine/threonine protein kinase is an important PH domain protein and its activity is required for normal preimplantation embryo development and survival. The activity of a range of PH domain proteins is also implicated in the normal development of the embryo. This review critically examines the evidence for the activation of 1-o-phosphatidylinositol 3-kinase in the generation of pleiotypic trophic response to embryotrophins in the autopoietic development of the preimplantation embryo.

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Section: Synthesis Of 3 0 -Phosphoinositidesmentioning

confidence: 72%

Section: Synthesis Of 3 0 -Phosphoinositidesmentioning

confidence: 99%

Phosphatidylinositol 3-kinase signaling in mammalian preimplantation embryo development

O’Neill

2008

Reproduction

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“…In mammalian cells, myo-inositol and its derivatives play important roles in reproduction (Chiu et al, 2003;Papaleo et al, 2009), embryo development (Kane et al, 1992;Greene and Copp, 1997;Hynes et al, 2000), and neuron function (Hanley et al, 1988;Fisher et al, 2002). Disruption of inositol homeostasis is reported to be a factor in bipolar disorder (Shaltiel et al, 2004) and neurodegenerative diseases, including Alzheimer's disease (Miller et al, 1993), Huntington's disease, and Parkinson's disease (Sarkar et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

d-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated Endomembrane Function inArabidopsisand Is Essential for Auxin-Regulated Embryogenesis

et al. 2011

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In animal cells, myo-inositol is an important regulatory molecule in several physiological and biochemical processes, including signal transduction and membrane biogenesis. However, the fundamental biological functions of myo-inositol are still far from clear in plants. Here, we report the genetic characterization of three Arabidopsis thaliana genes encoding D-myo-inositol-3-phosphate synthase (MIPS), which catalyzes the rate-limiting step in de novo synthesis of myo-inositol. Each of the three MIPS genes rescued the yeast ino1 mutant, which is defective in yeast MIPS gene INO1, and they had different dynamic expression patterns during Arabidopsis embryo development. Although single mips mutants showed no obvious phenotypes, the mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant were embryo lethal, whereas the mips1 mips3 and mips1 mips2 +/2 double mutants had abnormal embryos. The mips phenotypes resembled those of auxin mutants. Indeed, the double and triple mips mutants displayed abnormal expression patterns of DR5:green fluorescent protein, an auxin-responsive fusion protein, and they had altered PIN1 subcellular localization. Also, membrane trafficking was affected in mips1 mips3. Interestingly, overexpression of PHOSPHATIDYLINOSITOL SYNTHASE2, which converts myoinositol to membrane phosphatidylinositol (PtdIns), largely rescued the cotyledon and endomembrane defects in mips1 mips3. We conclude that myo-inositol serves as the main substrate for synthesizing PtdIns and phosphatidylinositides, which are essential for endomembrane structure and trafficking and thus for auxin-regulated embryogenesis.

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“…Inositol is essential for rabbit blastocyst expansion [30,31] and stimulates hatching of hamster blastocysts [32]. Furthermore, in bovine embryos, inositol is incorporated into phosphoinositides and inositol phosphates, inducing a very large change in the pattern of second messenger formation at the critical stage of blastocyst formation [33]. Holm et al [4] reported that the addition of a high myo-inositol concentration (2.77 mM) to SOF medium containing citrate and PVA enhanced bovine embryo development.…”

Section: Discussionmentioning

confidence: 99%

Establishment of An Advanced Chemically Defined Medium for Early Embryos Derived from In Vitro Matured and Fertilized Bovine Oocytes

Momozawa

Fukuda

2011

J. Reprod. Dev.

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Abstract.A chemically defined medium would be useful for analyzing promoters or inhibitors in in vitro culture (IVC) of bovine embryos. However, an IVC system for bovine embryos in a chemically defined medium has not been fully established. The present study was carried out to establish an advanced chemically defined medium for bovine embryos that supports a high rate of embryo development to the blastocyst stage. In the first experiment, we examined the effects of addition of Medium RD (RPMI1640 and Dulbecco's MEM, 1:1 v/v) to mKSOM/aa on developmental competence. The addition of 10% RD to mKSOM/aa with BSA improved the rate of development to the blastocyst stage; however, 10% RD-mKSOM/aa with PVP, which is a chemically defined medium, caused a reduction in the percentage of hatching blastocysts. In the second experiment, embryos were cultured in the chemically defined medium of 10% RD-mKSOM/ aa containing 11.7, 23.4, 46.8, 70.2 or 96.8 μM inositol. Inositol at the concentration of 70.2 μM improved the rate of development to the hatching blastocyst stage. In the third experiment, the optimal RD concentration in the IVC medium was evaluated. Embryos were cultured in the chemically defined medium supplemented with 10, 20 or 30% (v/v) RD. The rate of development to the blastocyst stage was highest with 20% RD. In the fourth experiment, the effects of Nacetylglucosamine (GlcNAc) as an IVC medium supplement on developmental competence were examined. The rate of development to the blastocyst stage with 1.0 mM GlcNAc was significantly higher than that without GlcNAc, but the rate of development with 1.2 mM GlcNAc was not different from that without GlcNAc. We also evaluated the ability of blastocysts produced in RD-mKSOM/aa to develop to normal calves after being transferred into recipients. Ten of the 16 recipients became pregnant, with 9 delivering normal calves. These results indicate that 20% RD-mKSOM/aa containing 70.2 μM myo-inositol and 1 mM GlcNAc is useful as a chemically defined medium for IVC of bovine embryos.Key words: Bovine embryos, Chemically defined medium, GlcNAc, Myo-inositol (J. Reprod. Dev. 57 : [681][682][683][684][685][686][687][688][689] 2011) I n vitro production (IVP) of bovine embryos by an in vitro maturation-fertilization culture system has been greatly improved. However, the rate of development of bovine oocytes to the blastocyst stage following maturation, fertilization and culture in vitro is limited to about 30-40% [1]. One possible reason for this is that embryo culture conditions, particularly the culture medium used for embryos, are suboptimal. Optimal conditions of a culture medium for embryos are 1) a high rate of embryo development to the blastocyst stage with repeatable results and 2) production of highquality blastocysts that have the competence to develop into competence to normal calves. In most studies on culture of mammalian embryos, the culture medium is supplemented with serum. A beneficial effect of serum on the development of bovine oocytes to the blastocyst stage ha...

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Uptake and incorporation ofmyo-inositol by bovine preimplantation embryos from two-cell to early blastocyst stages

Cited by 18 publications

References 29 publications

Phosphatidylinositol 3-kinase signaling in mammalian preimplantation embryo development

Phosphatidylinositol 3-kinase signaling in mammalian preimplantation embryo development

d-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated Endomembrane Function inArabidopsisand Is Essential for Auxin-Regulated Embryogenesis

Establishment of An Advanced Chemically Defined Medium for Early Embryos Derived from In Vitro Matured and Fertilized Bovine Oocytes

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