Uptake and molecular impact of aluminum-containing nanomaterials on human intestinal caco-2 cells

Sieg, Holger; Braeuning, Caroline; Kunz, Birgitta Maria; Daher, Hannes; Kästner, Claudia; Krause, Benjamin; Meyer, Thomas; Jalili, Pégah; Hogeveen, Kevin; Böhmert, Linda; Lichtenstein, Dajana; Burel, Agnès; Chevance, Soizic; Jungnickel, Harald; Tentschert, Jutta; Laux, Peter; Braeuning, Albert; Gauffre, Fabienne; Fessard, Valérie; Meijer, Jan; Estrela‐Lopis, Irina; Thünemann, Andreas F.; Luch, Andreas; Lampen, Alfonso

doi:10.1080/17435390.2018.1504999

Cited by 24 publications

(51 citation statements)

References 81 publications

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“…Therefore, to assess the biological effect of Ag NMs on intestinal barrier, the nanoma terials per se and the released silver ions should be considered together. [58,59] Apart from silver nanomaterials, CuO and ZnO nanomaterials also exhibited similar oxidative stress effects that induced cytotoxicity and damage to the ultrastructure of the intestine (Figure 5b). [60,61] Recently, Fe 2 O 3 NMs have been shown to disrupt the integ rity of intestinal barrier through affecting the expression of gammacatenin, Zona occludens1, and other integrity associated proteins in a sizedependent manner, in the order of 17 nm > 53 and 100 nm.…”

Section: Nano-iec Interaction and Subsequent Biological Effectsmentioning

confidence: 98%

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

Cui

Bao

Wang

et al. 2020

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201907665. Engineered nanomaterials (ENMs) are used in food additives, food packages, and therapeutic purposes owing to their useful properties, Therefore, human beings are orally exposed to exogenous nanomaterials frequently, which means the intestine is one of the primary targets of nanomaterials. Consequently, it is of great importance to understand the interaction between nanomaterials and the intestine. When nanomaterials enter into gut lumen, they inevitably interact with various components and thereby display different effects on the intestine based on their locations; these are known as location-oriented effects (LOE). The intestinal LOE confer a new biological-effect profile for nanomaterials, which is dependent on the involvement of the following biological processes: nanomucus interaction, nano-intestinal epithelial cells (IECs) interaction, nanoimmune interaction, and nano-microbiota interaction. A deep understanding ofNM-induced LOE will facilitate the design of safer NMs and the development of more efficient nanomedicine for intestine-related diseases. Herein, recent progress in this field is reviewed in order to better understand the LOE of nanomaterials. The distant effects of nanomaterials coupling with microbiota are also highlighted. Investigation of the interaction of nanomaterials with the intestine will stimulate other new research areas beyond intestinal nanotoxicity. www.advancedsciencenews.com

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Section: Nano-iec Interaction and Subsequent Biological Effectsmentioning

confidence: 98%

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

Cui

Bao

Wang

et al. 2020

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“…Particle dispersions were diluted freshly prior to use into serum-containing cell culture medium to adjust the desired concentrations and regular growth conditions. The cellular medium supernatant was then removed and the cells incubated with the prepared solutions for 24 or 48 h. In previous studies, particle stability in cell culture medie under incubation conditions was confirmed using analytical techniques like small-angle x-ray scattering (SAXS) or dynamic light scattering (DLS) (Sieg et al, 2018.…”

Section: Cell Cultivationmentioning

confidence: 98%

“…During the passage of the GI-tract, there occurs not only dissolution but also agglomeration / aggregation as well as particle formation from soluble Al species. The quantity of uptake and transport across the gastrointestinal barrier (of around 0.1 to 5 %) depends on the respective Al species (Sieg et al, 2018). This could result in a variety of particle-and ion-specific effects which need to be considered.…”

Section: Introductionmentioning

confidence: 99%

Aluminum in liver cells – the element species matters

et al. 2019

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Aluminum (Al) can be ingested from food and released from packaging and can reach key organs involved in human metabolism, including the liver via systemic distribution. Recent studies discuss the occurrence of chemically distinct Al-species and their interconversion by contact with biological fluids. These Al species can vary with regard to their intestinal uptake, systemic transport and therefore could have species-specific effects on different organs and tissues. This work aims to assess the in vitro hepatotoxic hazard potential of three different relevant Al species: soluble AlCl3 and two nanoparticulate Al species were applied, representing for the first time an investigation of metallic nanoparticles besides to mineral bound γ-Al2O3 on hepatic cell lines. To investigate the uptake and toxicological properties of the Al species, we used two different human hepatic cell lines: HepG2 and differentiated HepaRG cells. Cellular uptake was determined by different methods including light microscopy, transmission electron microscopy, side-scatter analysis and elemental analysis. Oxidative stress, mitochondrial dysfunction, cell death mechanisms and DNA damage were monitored as cellular parameters. While cellular uptake into hepatic cell lines occurred predominantly in the particle form, only ionic AlCl3 caused cellular effects. Since it is known, that Al species can convert one into another, and mechanisms including "trojan-horse"-like uptake can lead to an Al accumulation in the cells. This could result in the slow release of Al ions, for which reason further hazard cannot be excluded. Therefore, individual investigation of the different Al species is necessary to assess the toxicological potential of Al particles.

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“…In contrast, Al 2 O 3 is fully oxidized and shows a homogenous distribution of Al as well as oxygen on its surface. Our group was able to demonstrate that the protein corona that is formed during the contact of NMs with cell culture media is less complex for Al 0 NM compared to the protein corona of Al 2 O 3 NM [15]. The surface properties of NMs facilitate interactions with the surrounding environment, which also affects the bioavailability and the interactions of the NM with the cell.…”

Section: Cellular Uptakementioning

confidence: 98%

The Vitamin A and D Exposure of Cells Affects the Intracellular Uptake of Aluminum Nanomaterials and Its Agglomeration Behavior: A Chemo-Analytic Investigation

Kriegel

Krause

Reichardt

et al. 2020

IJMS

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Aluminum (Al) is extensively used for the production of different consumer products, agents, as well as pharmaceuticals. Studies that demonstrate neurotoxicity and a possible link to Alzheimer’s disease trigger concern about potential health risks due to high Al intake. Al in cosmetic products raises the question whether a possible interaction between Al and retinol (vitamin A) and cholecalciferol (vitamin D3) metabolism might exist. Understanding the uptake mechanisms of ionic or elemental Al and Al nanomaterials (Al NMs) in combination with bioactive substances are important for the assessment of possible health risk associated. Therefore, we studied the uptake and distribution of Al oxide (Al2O3) and metallic Al0 NMs in the human keratinocyte cell line HaCaT. Possible alterations of the metabolic pattern upon application of the two Al species together with vitamin A or D3 were investigated. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging and inductively coupled plasma mass spectrometry (ICP-MS) were applied to quantify the cellular uptake of Al NMs.

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Uptake and molecular impact of aluminum-containing nanomaterials on human intestinal caco-2 cells

Cited by 24 publications

References 81 publications

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

The Nano–Intestine Interaction: Understanding the Location‐Oriented Effects of Engineered Nanomaterials in the Intestine

Aluminum in liver cells – the element species matters

The Vitamin A and D Exposure of Cells Affects the Intracellular Uptake of Aluminum Nanomaterials and Its Agglomeration Behavior: A Chemo-Analytic Investigation

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