Uptake and transport of copolymer biodegradable microspheres by rabbit Peyer's patch M cells

Ermak, Thomas H.; Dougherty, Edward P.; Bhagat, Hitesh R.; Kabok, Zita; Pappo, Jacques

doi:10.1007/s004410050300

Cited by 16 publications

(15 citation statements)

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“…We found that the activity is unchanged after treatment with 0.1 M hydrochloric acid at 37°C for 4 h (data not shown). In addition, it is established that 1-10 m poly(D-Llactic-coglycolic acid) particles and polystyrene particles administered directly into the intestines following a laparotomy are readily internalized by Peyer's patch M cells (49,50). One would expect, therefore, that a part of the chitin particles given orally are taken up by M cells and translocated to the pocket region containing M and other leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Chitin Down-Regulates Serum IgE Levels and Lung Eosinophilia in the Allergic Mouse

Shibata¹,

Foster

Bradfield

et al. 2000

The Journal of Immunology

161

147

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Previous studies showed that local macrophages phagocytose nonantigenic chitin particles (1–10 μm polymers of N-acetyl-d-glucosamine) through mannose receptors and produce IL-12, IL-18, and TNF-α. These cytokines lead to the production of IFN-γ by NK cells. To determine whether chitin could down-regulate Th2 responses, chitin was given orally (8 mg/day for 3 days before and 13 days during ragweed allergen immunization) in BALB/c and C57BL/6 mice. These ragweed-immunized mice were given ragweed intratracheally on day 11. Three days after the challenge, the immunized mice with saline (controls) showed increases in serum IgE levels and lung eosinophil numbers. The chitin treatment resulted in decreases of these events in both strains. To dissect the inhibitory mechanisms of Th2 responses, spleen cells (4 × 106 cells/ml) isolated from the ragweed-immunized mice (controls) were cultured in the presence of ragweed and/or chitin for 3 days (recall responses). Ragweed alone stimulated the production of IL-4 (0.6 ng/ml), IL-5 (20 U/ml), and IL-10 (3.2 ng/ml), but not IFN-γ. Ragweed/chitin stimulation resulted in significant decreases of IL-4, IL-5, and IL-10 levels and the production of IFN-γ (48 U/ml). Moreover, spleen cells isolated from the chitin-treated mice showed ragweed-stimulated IFN-γ production (15 U/ml) and significantly lower levels of the Th2 cytokines, suggesting that the immune responses were redirected toward a Th1 response. Collectively, these results indicate that chitin-induced innate immune responses down-regulate Th2-facilitated IgE production and lung eosinophilia in the allergic mouse.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Chitin Down-Regulates Serum IgE Levels and Lung Eosinophilia in the Allergic Mouse

Shibata¹,

Foster

Bradfield

et al. 2000

The Journal of Immunology

161

147

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show abstract

“…It can be assumed that at least a subpopulation of these vesicles originates from the apical membrane to transport antigens from the lumen to the intercellular spaces by transcytosis. This transcellular route has been documented for tonsillar M-cells by the fluid-phase marker peroxidase (Gebert, 1995) and for M-cells of the intestine by various soluble and solid tracers (Ermak et al, 1995;Amerongen et al, 1992;Owen, 1977). Such substances and likewise complete microorganisms preferentially bind to the surface of M-cells (Sicinski et al, 1990;Inman and Cantey, 1983;Wolf et al, 1981), a process that is regarded as the initial step for their uptake or invasion.…”

Section: Discussionmentioning

confidence: 99%

M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes.

Gebert

1996

J Histochem Cytochem.

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The tonsil crypt epithelium contains membranous (M)-cells that transport antigens from the lumen to underlying lymphoid cells, thereby initiating specific immune responses. Mechanisms mediating the adhesion of antigens to the M-cell surface are important for effective and selective uptake of potential pathogens but are still poorly understood. Therefore, the carbohydrates present on crypt epithelial cells of the rabbit palatine tonsil were studied by lectin histochemistry. Ultrathin LR White sections were incubated with a panel of eight lectins conjugated to colloidal gold or biotin. The glycocalyx of the apical membrane of M-cells was selectively labeled by UEA-I, LTA, HPA, and VVA, whereas that of the remaining squamous epithelial cells preferentially bound RCA-I and PNA. WGA and ConA showed only little binding, with no discernible preference for any of the cell types. Double labeling of UEA-1 together with anti-vimentin antibodies revealed that UEA-I-positive epithelial cells also contained the rabbit M-cell marker vimentin, and vice versa. The results show that a specific composition of glycoconjugates, which differs from that on squamous epithelial cells, is found on M-cells of the rabbit tonsil. The M-cell-specific glycoproteins and glycolipids could be selectively targeted by microorganisms that adhere to M-cells and enter the host along this pathway.

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“…M cells have the ability to transport bacteria, [15][16][17][18] viruses, [19][20][21][22][23][24] parasites 25 and non-infectious particles 26 through the apical membrane to the basolateral surface. When bacteria and large particles get into the mucosal lumen, M cells undergo apical membrane ruffling, actin cytoskeleton rearrangements, 27 and interdigitation, and then phagocytose these large particles.…”

Section: Function Of M Cellsmentioning

confidence: 99%

Roles of M cells in infection and mucosal vaccines

Wang

Gao

Zhang

et al. 2014

Human Vaccines & Immunotherapeutics

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Uptake and transport of copolymer biodegradable microspheres by rabbit Peyer's patch M cells

Cited by 16 publications

References 0 publications

Oral Administration of Chitin Down-Regulates Serum IgE Levels and Lung Eosinophilia in the Allergic Mouse

Oral Administration of Chitin Down-Regulates Serum IgE Levels and Lung Eosinophilia in the Allergic Mouse

M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes.

Roles of M cells in infection and mucosal vaccines

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