Uptake, distribution and metabolism of 6-Benzyladenine and cytokinin content during callus initiation from actinidia deliciosa tissues

Centeno, Marı́a Luz; Rodrı́guez, Ana; Albuerne, M.; Feito, Isabel; Fernández, Belén

doi:10.1016/s0176-1617(98)80267-8

Cited by 7 publications

(14 citation statements)

References 22 publications

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“…Contrasting with the role proposed for NAA and IAA, in a previous report (Centeno et al 1998) we established that BA and natural cytokinins are more involved in the onset than in the maintenance of disorganized growth of kiwifruit callus because they reached their highest tissue levels at 24 and 48 h of CIM culture, coinciding with first cell divisions, and decreased afterwards. These observations are also supported by the BA/NAA ratio, its maximum value being measured at 24 h (Table 2).…”

Section: Discussioncontrasting

confidence: 88%

“…Cellular division surrounding the wounded tissues took place either in apical or basal petiolar ends, although the proliferation led to callus development in the basal extreme alone. This callogenic response has been shown in previous reports (Centeno et al 1996(Centeno et al , 1998: the beginning of cell division occurred within the first 96 h of CIM culture and differences among the two petiolar edges were visible for 10 days, when the basal callus was already in a developing state. We also studied the uptake, metabolism, and levels of BA in kiwifruit explants during the earlier period of culture (Centeno et al 1998).…”

Section: Discussionsupporting

confidence: 83%

“…3), which confirms again conjugation as the main metabolic path for regulation of levels of exogenously provided auxins to plant tissues Centeno et al (1996). b Centeno et al (1998). (Kleczkowski and Schell 1995).…”

Section: Discussionsupporting

confidence: 57%

“…Once the free NAA achieved a level near 2 nmol g −1 dry weight at 12 h inside the explants, the hormone was present in similar amounts during the remaining period of study. Taking into account that the fresh weight/dry weight ratio for kiwifruit petioles was 8 ± 0.02, these data could be represented as endogenous concentrations of free NAA (Table 2), being almost equal to the initial exogenously applied NAA concentration for 12 h. Table 2 also shows the relationships between the free NAA and free BA concentrations in kiwifruit explants during the culture period of callus initiation, the cytokinin concentration being established in a previous work (Centeno et al 1998). The BA/NAA ratio was favorable for the cytokinin in kiwifruit tissues, as well as in the medium at the initial time, but changed during CIM culture.…”

Section: Concentration Of Naa In Kiwi Tissues-relationships Between Nmentioning

confidence: 80%

“…An effort to explain the role of cytokinins in this callogenic response culminated in an established positive correlation between the content of this type of PGR in kiwifruit tissues, mainly BA, and the induction and initiation of basal callus; the onset of cell division, which occurred within the first 96 h of culture on callus induction medium (CIM), was preceded by a high free BA level found in the tissues at 24 and 48 h (Centeno et al 1998). In relation to the synthetic auxin applied on CIM, it is known that NAA is conjugated into a glucose ester and several amino acid amides.…”

Section: Indoleacetic Acid-kiwifruit-naphthaleneacetic Acidmentioning

confidence: 99%

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Uptake, Distribution, and Metabolism of 1-Naphthaleneacetic Acid and Indole-3-Acetic Acid During Callus Initiation From Actinidia deliciosa Tissues

Centeno

Fernández

Feito

et al. 1999

J Plant Growth Regul

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1-Naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) were required for in vitro callus formation at the basal edge of kiwifruit (Actinidia deliciosa [A. Chev] Liang and Ferguson, cv. Hayward) petioles. The uptake, metabolism, and concentration of NAA and indole-3-acetic acid (IAA) content were examined in the explants during the callus initiation period. After 1, 6, 12, 24, 48, and 96 h of culture in the presence of [H(3)]NAA, petioles were divided into apical, middle, and basal portions and analyzed. Except for a high IAA level measured at 12 h, IAA content decreased in tissues during a culture period of 96 h. NAA uptake was higher in petiolar edges than in the middle portion, and NAA was rapidly conjugated with sugars and aspartic acid inside the tissues. The amide conjugation was triggered in apical and basal portions from 12 h and in the middle part from 48 h, with alpha-naphthylacetylaspartic acid being the major metabolite. Free-NAA concentration in cultured petioles achieved an equilibrium with the exogenously applied NAA (0.27 µm) from 12 h, and it remained constant thereafter. The relationships between the role attributed to NAA and BA in the initiation and the maintenance of disorganized growth of callus in kiwifruit cultures are discussed.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 57%

Section: Concentration Of Naa In Kiwi Tissues-relationships Between Nmentioning

confidence: 80%

Section: Indoleacetic Acid-kiwifruit-naphthaleneacetic Acidmentioning

confidence: 99%

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Uptake, Distribution, and Metabolism of 1-Naphthaleneacetic Acid and Indole-3-Acetic Acid During Callus Initiation From Actinidia deliciosa Tissues

Centeno

Fernández

Feito

et al. 1999

J Plant Growth Regul

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Micropropagation of Kiwifruit (Actinidia spp.)

Rugini

Gutiérrez-Pesce

2003

Micropropagation of Woody Trees and Fruits

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Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation

Prado

González²,

Romo³

et al. 2006

Plant Cell Tiss Organ Cult

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The present work reports on a study of plant regeneration carried out with callus from the leaf blades and petioles of field-grown male adult kiwifruit plants (Actinidia deliciosa (Chev.) Liang and Ferguson). The cultivars used were 'Tomuri' and clone A, a selected male plant grown in north western Spain. The best shoot induction conditions were obtained in 'Tomuri' leaf blades cultured in K(h) medium in the presence of 23 lM Zeatin and 0.1 lM NAA. Under these conditions, more than 80% of organogenic callus induction was observed, with an average of 14 new shoots in the second subculture. The initial length of the shoots affected shoot elongation, which was accomplished by culturing isolated shoots in K(h) medium with half-strength salts, supplemented with 0.4 lM Zeatin and 0.1 lM NAA. A possible detrimental long-term effect of cytokinins on shoot elongation can account for the results, since elongation was not observed until 1 month of culture in elongation medium.For rooting, shoots (1 cm in length) were basally immersed in a 5 mM IBA solution for 15 s, and transferred to half-strength K(h) basal medium. Regenerated plants were acclimated in a sterile peat:perlite substrate for 10 days, and then transferred to soil. AFLP analysis was accomplished with 15 primer combinations from which 13 showed reproducible and well-resolved bands, producing a total of 1321 fragments from which 1281 were polymorphic (97%). A dendrogram was constructed using both monomorphic and polymorphic bands, showing genetic variation among field-grown plants and tissue culture-derived regenerants.

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Uptake, distribution and metabolism of 6-Benzyladenine and cytokinin content during callus initiation from actinidia deliciosa tissues

Cited by 7 publications

References 22 publications

Uptake, Distribution, and Metabolism of 1-Naphthaleneacetic Acid and Indole-3-Acetic Acid During Callus Initiation From Actinidia deliciosa Tissues

Uptake, Distribution, and Metabolism of 1-Naphthaleneacetic Acid and Indole-3-Acetic Acid During Callus Initiation From Actinidia deliciosa Tissues

Micropropagation of Kiwifruit (Actinidia spp.)

Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation

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