Uptake of ciprofloxacin by human neutrophils

Easmon, C. S. F.; Crane, J. P.

doi:10.1093/jac/16.1.67

Cited by 131 publications

(88 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ofloxacin seems to possess the best properties: levels in tissues and elimination half-lives are higher. Moreover, as mentioned above, these drugs penetrate well into mammalian cells (14,15). These drugs could be useful in the treatment of tuberculosis and other mycobacterioses with slowly and rapidly growing mycobacteria, including M. leprae.…”

Section: Commentsmentioning

confidence: 98%

See 1 more Smart Citation

Mycobacteria and the new quinolones

Leysen

Haemers

Pattyn

1989

Antimicrob Agents Chemother

148

View full text Add to dashboard Cite

Section: Commentsmentioning

confidence: 98%

“…Moreover, these compounds appear to penetrate readily into mammalian cells. This property is important for the treatment of intracellular pathogens, such as Mycobacterium tuberculosis (14,15).…”

Section: Introductionmentioning

confidence: 99%

Mycobacteria and the new quinolones

Leysen

Haemers

Pattyn

1989

Antimicrob Agents Chemother

148

View full text Add to dashboard Cite

“…Fluoroquinolones have long been known to accumulate in eucaryotic cells [15,[33][34][35][36][37][38][39][40]. The cellular concentrations of fluoroquinolones are generally 4-to 10-fold larger than the extracellular.…”

Section: Fluoroquinolonesmentioning

confidence: 99%

Intracellular pharmacodynamics of antibiotics

Carryn

Chanteux

Seral

et al. 2003

Infectious Disease Clinics of North America

175

151

View full text Add to dashboard Cite

“…It has been postulated that the favorable clinical results observed with rifampin combinations are due to the ability of rifampin to concentrate within phagocytic cells and kill intracellular staphylococci (16). Resistance to the fluoroquinolones has also been observed during treatment of patients with difficult-to-eradicate staphylococcal infections (5,10), and these drugs, like rifampin, are concentrated in human neutrophils where they are lethal to staphylococci (6). The fluoroquinolones have been consistently active in vitro against the most common species of clinically significant staphylococci.…”

Section: Methodsmentioning

confidence: 99%

In vitro antistaphylococcal activity of pefloxacin alone and in combination with other antistaphylococcal drugs

Fass

Helsel

1987

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Despite the availability of numerous antistaphylococcal drugs, the treatment of severe staphylococcal infections remains a major therapeutic challenge. A new class of antimicrobial agents, the fluoroquinolones, is under development. These drugs are consistently active against both methicillin-or oxacillin-susceptible (OS) and methicillin-or oxacillin-resistant (OR) staphylococci (2,8,9,20,26). In this study, we compared the in vitro antistaphylococcal activity of one fluoroquinolone, pefloxacin, alone and in combination with other drugs, with the antistaphylococcal activities of other drugs and drug combinations.MATERIALS AND METHODS Organisms. Staphylococci were clinical isolates identified by using a profile of 27 biochemical tests and the interpretive criteria of Kloos and Schleifer (13). Included were 50 strains each of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus haemolyticus and 25 strains each of Staphylococcus hominis and Staphylococcus saprophyticus. Half of the S. aureus, S. epidermidis, and S. haemolyticus strains and all of the S. hominis and S. saprophyticus strains were OS; the others were OR.MICs. Laboratory standards of oxacillin, cephalothin, pefloxacin, vancomycin, gentamicin, erythromycin, clindamycin, doxycycline, and trimethoprim-sulfamethoxazole were supplied by the manufacturers, diluted according to instructions, and dispensed into microdilution plates using an MIC-2000 Plus dispensing machine (Dynatech Laboratories, Inc., Alexandria, Va.) in log2 dilution steps within the range of 0.06 to 64 ,ug/ml. MICs were determined by a standardized microdilution method (18) in 0.1-ml volumes of cation-supplemented Mueller-Hinton broth (Difco Laboratories, Detroit, Mich.). For oxacillin and cephalothin, 2% NaCl was added to the medium (18). Trimethoprimsulfamethoxazole was tested in a fixed 1:19 ratio; 0.1 U of thymidine phosphorylase (Burroughs Wellcome Co., Re-* Corresponding author. search Triangle Park, N.C.) per ml was added. The final inoculum was ca. 5 x 105 CFU/ml. The trays were incubated at 35°C. MIC endpoints were determined at 18 h; oxacillin susceptibility or resistance was determined by using appropriate breakpoints for the various species tested (9).To screen for synergy or antagonism, MICs were determined for 10 strains each of OS S. aureus, OR S. aureus, OS S. epidermidis, OR S. epidermidis, OS S. haemolyticus, and OR S. haemolyticus by using fixed 1:1 (by weight) drug combinations: oxacillin with pefloxacin, gentamicin, or rifampin against OS strains; vancomycin with pefloxacin, gentamicin, or rifampin against OR strains; and pefloxacin with gentamicin or rifampin against all strains.Killing kinetics. Killing-kinetic studies were performed with pefloxacin, oxacillin, vancomycin, rifampin, gentamicin, and various combinations of those drugs with 23 strains of S. aureus, S. epidermidis, and S. haemolyticus. Studies were performed in 50-ml volumes of cationsupplemented Mueller-Hinton broth with an inoculum of ca.106 CFU/ml. Antibiotic concentrations were selec...

show abstract

Uptake of ciprofloxacin by human neutrophils

Cited by 131 publications

References 0 publications

Mycobacteria and the new quinolones

Mycobacteria and the new quinolones

Intracellular pharmacodynamics of antibiotics

In vitro antistaphylococcal activity of pefloxacin alone and in combination with other antistaphylococcal drugs

Contact Info

Product

Resources

About