Uptake of foreign ferritin in platy, Xiphophorus maculatus (Poeciliidae: Teleostei)

Leknes, Ingvar Leiv

doi:10.3354/dao051233

Cited by 16 publications

(13 citation statements)

References 20 publications

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“…In this study, we demonstrated that more than 90% of the iron, injected as IDx, into the peritoneal cavity of the sea bass (Dicentrarchus labrax L.), was absorbed within 6 h. This is similar to what has been observed in rats (Suzuki et al 1995) and in other fish species (Leknes 2001(Leknes , 2002). …”

Section: Discussionsupporting

confidence: 74%

A model for acute iron overload in sea bass (Dicentrarchus labrax L.)

Rodrigues

Pereira

2004

Lab Anim

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SummaryEvaluation of several parameters involved in iron metabolism was carried out after intraperitoneal (i.p.) injection with iron dextran (IDx) in sea bass (Dicentrarchus labrax L.). After treatment, a rapid mobilization of IDx from the peritoneal cavity to other organs was observed. This was followed by a modification of normal peripheral blood iron parameters. Total iron (TI) and transferrin saturation (TS) rose rapidly, to 4.14 g/ml and 83.7%, respectively, on day 3. In contrast, unsaturated iron binding capacity (UIBC) dropped from 3.19 g/ml (at day 0) to 0.90 g/ml on day 3. Tissue iron content was determined by atomic absorption spectometry (AAS). Three days post-IDx injection, values of iron concentration in liver, spleen and head kidney were significantly higher than control values (15, 6 and 9-fold increase, respectively). Samples of liver, spleen and head kidney were processed for routine histology, and the Perl's method was used for iron staining. Histological sections of the IDx-treated animals showed iron deposition in all tissues studied. In the liver, the iron was evenly distributed over the whole organ, being present in the hepatocytes. In the head kidney and spleen, the iron deposition was mainly observed in the melanomacrophage centres (MMCs). The present study characterizes several parameters involved in iron metabolism, and develops a fish model, of iron overload, which can be used in further studies of iron toxicity and iron-induced susceptibility to bacterial infections.

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Section: Discussionsupporting

confidence: 74%

A model for acute iron overload in sea bass (Dicentrarchus labrax L.)

Rodrigues

Pereira

2004

Lab Anim

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“…Recent studies have revealed that the heart atrial and ventricular endocardial cell layers in gadoid and poecilid species are able to take up large amounts of scavenger or foreign macromolecules from the blood stream (Smedsrød et al 1995;Dalmo et al 1996Dalmo et al ,1997Sørensen et al 1997Sørensen et al , 1998Seternes et al 2002;Leknes 1987Leknes , 2002. A similar heart endocardial uptake has also been noted by Ferguson (1975) in Pleuronectes platessa Linnaeus, 1758 (European plaice) and by Nakamura and Shimozawa (1994) in Oryzias latipes (Temminck and Schlegel, 1846) (Japanese rice Wsh).…”

Section: Discussionmentioning

confidence: 65%

“…However, as these cells are Xat in this species, but tall in P. virens and X. maculatus, the amounts of accumulated materials are much less in T. leerii than in the two latter species (Leknes 1987(Leknes , 2002.…”

Section: Discussionmentioning

confidence: 90%

“…These cells seem to play important blood cleansing roles in Gadus morhua Linnaeus, 1758 (Atlantic cod) and Xiphophorus maculatus (Günther, 1866) (Southern platyWsh) (Smedsrød et al 1995;Dalmo et al 1996;Sørensen et al 1998;Seternes et al 2002;Leknes 2002). Previously, electron microscopical studies have shown that intraperitoneally injected horse-spleen ferritin is taken up and tightly packed in huge membrane-bound bodies in such cells in heart of Pollachius virens (Linnaeus, 1758) (Pollock) and X. maculatus, and that large amounts of Prussian blue precipitate in the ferritin-Wlled endocardial bodies in platyWsh heart after treatment with acid ferrocyanide (Leknes 1987(Leknes , 2002. Blood cleansing in heart of anabantid teleosts has not been investigated previously, as far as we know.…”

Section: Introductionmentioning

confidence: 97%

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Blood cleansing in heart atrium of Trichogaster leerii (Bleeker, 1852), Anabantidae: Teleostei

Leknes

2007

Zoomorphology

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The capability and capacity of endocardial tissue in heart of Trichogaster leerii (Pearl gourami) to take up intraperitoneally injected horse-spleen ferritin and the regular content of indigestible wastes in this tissue are described. The entire heart wall was spongy composed of muscle trabeculae enveloped by Xat endocardial cells. In atrium these cells were Wlled by Prussian blue precipitations in sections treated with acid ferrocyanide, when the period of time between ferritin-injection and sacriWce was 6 h or more. The endocardial cell layers enveloping the muscle trabeculae in ventricle or covering the wall and valves of the ventricular apertures nearly lacked such precipitations. Those endocardial cell layers capable to endocytose much foreign ferritin, also reacted strongly with Schmorl's solution in control hearts, i.e. these atrial cells are normally rich in indigestible wastes, probably lipofuscin. We suggest that the endocytic and lipofuscin-rich endocardial cells in heart atrium may play a role in the blood clearance in T. leerii and that this cleansing in heart is restricted to atrium only in this species.

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“…it consists of muscle trabeculae which are enveloped by endocardial cell layers with significant blood cleansing capabilities (Leknes 1987(Leknes , 2002Smedsrød et al 1995;Sørensen et al 1998). Previously, electron microscopical studies have shown that these cells are mainly filled by moderately electron dense bodies and that intraperitoneally injected horse-spleen ferritin is taken up by this type of cells and packed in huge membrane-bound bodies (Leknes 1980(Leknes , 1987.…”

Section: Introductionmentioning

confidence: 98%

Lipofuscin-like pigments in endocardial cells enveloping the heart muscle trabeculae in platyfish (Poeciliidae: Teleostei)

Leknes

2007

Fish Physiol Biochem

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Large amounts of lipofuscin-like pigments were demonstrated by means of the Schmorl's technique in endocardial cells enveloping the heart muscle trabeculae in adult platyfish (Xiphophorus maculatus). Such pigment granules occurred in a low number in the corresponding cell layers in heart of 6 mm long prenatal platies. The endocardial cells contained numerous lysosome-like bodies, bristle-coated vesicles and tubules of agranular endoplasmic reticulum, particularly in adult specimens. The lipofuscin-rich endocardial cell layers displayed deep blue precipitations in hearts from the ferritin-injected adult platies 6 h or more post-injection, when treated with acid ferrocyanide instead of Schmorl's solution, i.e. this cell type is able to take up foreign particles. The occurrence of much lipofuscin-like pigments in endocardial cell layers in adult platies provides evidence for that these cells have performed important scavenger functions at this stage of development.

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Uptake of foreign ferritin in platy, Xiphophorus maculatus (Poeciliidae: Teleostei)

Cited by 16 publications

References 20 publications

A model for acute iron overload in sea bass (Dicentrarchus labrax L.)

A model for acute iron overload in sea bass (Dicentrarchus labrax L.)

Blood cleansing in heart atrium of Trichogaster leerii (Bleeker, 1852), Anabantidae: Teleostei

Lipofuscin-like pigments in endocardial cells enveloping the heart muscle trabeculae in platyfish (Poeciliidae: Teleostei)

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