Uptake of yolk proteins in Rhodnius prolixus

Oliveira, Pedro L.; Gondim, Kátia C.; Guedes, Damião Maciel; Masuda, Hatisaburo

doi:10.1016/0022-1910(86)90101-0

Cited by 70 publications

(42 citation statements)

References 39 publications

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“…Activation of these enzymes is achieved by luminal acidification of YGs, a process that is mediated by proton pumps (Fagotto, 1991;Nordin et al, 1991;Mallya et al, 1992;Fagotto, 1995). VT and hydrolases, such as cathepsin B and carboxypeptidase, are known to accumulate in the oocytes during oogenesis and to be stored in YGs (Wall and Meleka, 1985;Oliveira et al, 1986;Raikhel and Dadhialla, 1992;Valle, 1993;Sappington and Raikhel, 1998;Cho et al, 1991;Cho et al, 1999;Liu and Nordin, 1998;Yin et al, 2001). Newly laid eggs are therefore able to initiate VT degradation.…”

Section: Discussionmentioning

confidence: 99%

“…Endocytic activities during insect oogenesis involve the incorporation of the lipophosphoglycoprotein vitellogenin (Oliveira et al, 1986;Raikhel and Dadhialla, 1992;Valle, 1993;Sappington and Raikhel, 1998). Once inside the oocyte the vitellogenin, now referred to as vitellin (VT), is stored in organelles known as yolk granules (YGs) (Kunkel and Nordin, 1985;Purcell et al, 1988;Machado et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Calcium-regulated fusion of yolk granules is important for yolk degradation during early embryogenesis of Rhodnius prolixusStahl

Ramos

Miranda

Souza

et al. 2007

Journal of Experimental Biology

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SUMMARY This study examined the process of membrane fusion of yolk granules (YGs)during early embryogenesis of Rhodnius prolixus. We show that eggs collected at days 0 and 3 after oviposition contain different populations of YGs, for example day-3 eggs are enriched in large YGs (LYGs). Day-3 eggs also contain the highest free [Ca2+] during early embryogenesis of this insect. In vitro incubations of day-0 YGs with [Ca2+]similar to those found in day-3 eggs resulted in the formation of LYGs, as observed in vivo. Fractionation of LYGs and small YGs (SYGs) and their subsequent incubation with the fluorescent membrane marker PKH67 showed a calcium-dependent transference of fluorescence from SYGs to LYGs, possibly as the result of membrane fusion. Acid phosphatase and H+-PPase activities were remarkably increased in day-3 LYGs and in calcium-treated day-0 LYGs. Both fractions were found to contain vitellins as major components, and incubation of YGs with calcium induced yolk proteolysis in vitro. Altogether, our results suggest that calcium-induced membrane fusion events take part in yolk degradation, leading to the assembly of the yolk mobilization machinery.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Calcium-regulated fusion of yolk granules is important for yolk degradation during early embryogenesis of Rhodnius prolixusStahl

Ramos

Miranda

Souza

et al. 2007

Journal of Experimental Biology

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“…Various binding and uptake studies have indicated that Vg is internalized via receptor-mediated endocytosis (RME), and have suggested the presence of specific Vg receptors in the oocytes of A. aegypti , L. migratoria (Rö hrkasten and Ferenz, 1985;Rö hrkasten et al, 1989), Nauphoeta cinerea (Kö nig and Lanzrein, 1985), Blattella germanica (Kö nig et al, 1988), R. prolixus (Oliveira et al, 1986), M. sexta , and H. cecropia (Kulakosky and Telfer, 1987). However, despite these pioneering studies, the mechanisms of endocytosis have been largely elucidated in other systems, by a combination of animal biochemistry and cell biology, and yeast genetics.…”

Section: P0415mentioning

confidence: 99%

Vitellogenesis and Post-Vitellogenic Maturation of the Insect Ovarian Follicle

Swevers

2005

Comprehensive Molecular Insect Science

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“…The VG of R. prolixus is synthesized in the fat body as a high molecular mass precursors of 205 kDa and 190 kDa (Valle et al 1993). VG is secreted to the hemolymph and it is taken up by the growing oocytes at the ovary by receptor mediated endocytosis (Oliveira et al 1986). Inside the eggs VG is referred to as VT to differentiate it from VG from the hemolymph.…”

Section: Vitellins and Vitellogeninsmentioning

confidence: 99%

Oogenesis and egg development in triatomines: a biochemical approach

Atella

Gondim

Machado

et al. 2005

An. Acad. Bras. Ciênc.

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In triatomines, as well as in other insects, accumulation of yolk is a process in which an extra-ovarian tissue, the fat body, produces yolk proteins that are packed in the egg. The main protein, synthesized by the fat body, which is accumulated inside the oocyte, is vitellogenin. This process is also known as vitellogenesis. There are growing evidences in triatomines that besides fat body the ovary also produces yolk proteins. The way these yolk proteins enter the oocyte will be discussed. Yolk is a complex material composed of proteins, lipids, carbohydrates and other minor components which are packed inside the oocyte in an organized manner. Fertilization triggers embryogenesis, a process where an embryo will develop. During embryogenesis the yolk will be used for the construction of a new individual, the first instar nymph. The challenge for the next decade is to understand how and where these egg proteins are used up together with their non-protein components, in pace with the genetic program of the embryo, which enables cell differentiation (early phase of embryogenesis) and embryo differentiation (late phase) inside the egg.

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Uptake of yolk proteins in Rhodnius prolixus

Cited by 70 publications

References 39 publications

Calcium-regulated fusion of yolk granules is important for yolk degradation during early embryogenesis of Rhodnius prolixusStahl

Calcium-regulated fusion of yolk granules is important for yolk degradation during early embryogenesis of Rhodnius prolixusStahl

Vitellogenesis and Post-Vitellogenic Maturation of the Insect Ovarian Follicle

Oogenesis and egg development in triatomines: a biochemical approach

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