Uptake of zinc from zinc sulfate and zinc proteinate by ovine ruminal and omasal epithelia1,2

Abstract

Uptake and transport of Zn from (65)Zn-labeled ZnSO(4) and Zn proteinate (ZnProt) by ruminal and omasal epithelia were examined by using a parabiotic chamber system. Uptake was measured during a 4-h incubation with 10, 20, or 200 microM Zn as ZnSO(4) or ZnProt in the mucosal buffer (pH 6.0, Krebs-Ringer phosphate). Zinc uptake and transport were also evaluated after simulated ruminal digestion. Buffered ruminal fluid contained a feed substrate and 10 or 200 microM added Zn as ZnSO(4) or ZnProt. In a preliminary experiment, uptake of Zn by omasal tissue was low; thus, the remaining experiments were conducted solely with ruminal epithelium. Incubations to determine the effect of time on Zn uptake from mucosal buffer containing 20 microM added Zn as ZnSO(4) or ZnProt resulted in increased (P < 0.01) Zn uptake as incubation time increased from 30 to 240 min. Zinc uptake was also greater (P = 0.02) from mucosal buffer containing ZnProt compared with ZnSO(4). Zinc uptake from incubations containing 10 or 200 microM was affected by source x concentration (P = 0.05) and concentration x time (P < 0.01) interactions. With 10 microM Zn, uptake was not influenced by Zn source, whereas when 200 microM Zn was added, Zn uptake from ZnProt was greater than from ZnSO(4). Increasing incubation time resulted in increased Zn uptake with 200 microM Zn in the mucosal buffer; however, with 10 microM Zn, uptake did not change after 30 min. After simulated ruminal fermentation, the proportion of Zn in a soluble form was influenced by a source x concentration interaction (P = 0.03). After 18 h of incubation, the proportion of Zn that was soluble was not different between ZnProt and ZnSO(4) in buffered ruminal fluid that contained 10 microM added Zn, but was greater for ZnProt compared with ZnSO(4) with 200 microM Zn in the incubation. Zinc uptake from the aqueous fractions of simulated ruminal digestions containing 200 microM added Zn was greater (P < 0.01) than from those containing 10 microM added Zn. Zinc transport, based on detection of (65)Zn in serosal buffer, did not occur in any of the experiments. The results of the current experiments suggest that absorption of Zn into the bloodstream does not occur from the ruminant foresto-mach; however, Zn uptake occurs in ruminal tissue and is greater from ZnProt than from ZnSO(4).

Effects of peroral supplementation of different forms of zinc on the ruminal mucosa of goat kids – a morphometric study

“…Similar results were reported also by other authors (Puchala et al 1999;Zitnan et al 2003;Arelovich et al 2008;Wright et al 2008).…”

“…various inorganically and organically bound forms; in veterinary and human medicine it is also used for its antibacterial effect (Puchala et al 1999;Wright et al 2008;Pechová et al 2006Pechová et al , 2009Screenivasan et al 2009). There is absence of literature describing the effects of oral supplementation of different forms of zinc on ruminal mucosa of kids.…”

New Aspects of Therapy of Hepatocellular Carcinoma Egyptian Patients

“…Moreover, Also, Wright et al [86] they proved that trace elements as zinc showed restoration towards normal level after natural oils supplementation due to improved disease state since the cause affecting their changes were diminished. Supplementation of Zn, during times of oxidative stress may reduce oxidative damage to white blood cells and increase disease resistance.…”

Evaluation of bioavailability of selected minerals from Maize Germ Oilcake in crossbred male calves

“…The mineral content can be determined chemically whereas bioavailability is much more difficult to be estimated (Singh, 2013). The majority of minerals are absorbed only minimally across the rumen epithelium and they cannot be absorbed by the animal until they reach the small intestine (Wright et al, 2008). Dissolved minerals in the reticulorumen, omasum, and abomasum can form indigestible compounds with polyphenols, phytates, oxalates and some sugars that pass into the manure as indigestible waste (Spears, 2003).…”