Uptake of β-phenylethylamine in rat isolated lung

Ben-Harari, Ruben R.; Bakhle, Y.S.

doi:10.1016/0006-2952(80)90367-6

Cited by 21 publications

(15 citation statements)

References 17 publications

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“…Since uptake of PEA was inhibited as early as 37 h after exposure to 02 and uptake of two other amines, 5-HT and noradrenaline, is known to occur in pulmonary endothelial cells (Strum & Junod, 1972;Nicholas, Strum, Angelo & Junod, 1974;Cross, Alabaster, Bakhle & Vane, 1974), the site of uptake and metabolism of PEA in lung may be the capillary endothelium. Uptake of PEA into lung comprises a saturable component, suggesting a specific site of uptake, and a large passive component (Ben-Harari & Bakhle, 1980). If hyperoxia is inhibiting the uptake of PEA at the specific site it would suggest that the site of uptake of PEA on the endothelium is different from that for 5-HT.…”

Section: Discussionmentioning

confidence: 99%

“…The methods used to measure uptake and metabolism in perfused lungs, to prepare the homogentate and to assay deamination were those described previously (Tipton & Youdim, 1976;Ben-Harari & Bakhle, 1980 General effects of 02 exposure Rats exposed to 100% 02 at 1 ATA for up to 40 h appeared well and were indistinguishable on inspection from air-exposed control rats. After 40 h exposure the rats became lethargic and suffered from slight respiratory distress.…”

Section: Preparation Of Lungsmentioning

confidence: 99%

“…Monoamine oxidase activity in perfused lung and in homogenates When the metabolite in the lung is expressed as a proportion of the total radioactivity retained by the lung, these values have been shown to reflect accurately MAO activity of the lung (Ben-Harari & Bakhle, 1980). Analysis of radioactivity retained by the lung from female rats showed that during 02 exposure, MAO activity towards 5-HT was unchanged (Table 1), but towards PEA, MAO activity appeared to increase after hyperoxia although this did not become statistically significant until after 37 h. To confirm these results obtained in perfused lung and in order to determine MAO activity directly, the deamination of ["4C]-5-HT (0.5 mM) and ['4C]-PEA (0.05 mM) was measured in homogenates of lungs from female rats.…”

Section: Uptake In Isolated Perfused Lungsmentioning

confidence: 99%

“…Pulmonary metabolism of 5-HT and f3-phenylethylamine (PEA) consists of uptake into cells and deamination by the enzyme, monoamine oxidase (MAO). The uptake systems for both 5-HT and PEA in lung have been studied (Junod, 1972;Ben-Harari & Bakhle, 1980) Youdim & Lanir, 1980).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Hyperoxia on Uptake and Metabolism of 5‐hydroxytryptamine and Β‐phenylethylamine in Rat Lung: A Sex Difference

Ben-Harari

Lanir

Youdim

1981

British J Pharmacology

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1 The uptake of 5-hydroxytryptamine (5-HT) and /8-phenylethylamine (PEA) and their deamination by monoamine oxidase (MAO) were studied in perfused lung from male and female rats exposed to 100% 02 at 1 ATA for up to 60 h. 2 The uptake and metabolism of 5-HT in lungs from both male and female rats was not changed by exposure to 02-3 The uptake and metabolism of PEA by lungs from male rats was unchanged. Uptake of PEA by lungs from female rats was inhibited 20% and 62% after 37 h and 50 h exposure respectively. 4 MAO activity, both in vitro and in perfused lung, was increased towards PEA after 35 h of hyperoxia. 5 Metabolism of PEA in perfused lung, measured over 30 min, was inhibited 52% after 50 h of 02 hyperoxia. 6 These results show that exposure to high concentrations of 02 damages lung, resulting in inhibition of uptake of PEA and consequently in inhibition of metabolism of PEA.7 These results also indicate that, in lung from female rats, MAO-type B is more susceptible to changes in 02 tension than MAO type A.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Lungsmentioning

confidence: 99%

Section: Uptake In Isolated Perfused Lungsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Hyperoxia on Uptake and Metabolism of 5‐hydroxytryptamine and Β‐phenylethylamine in Rat Lung: A Sex Difference

Ben-Harari

Lanir

Youdim

1981

British J Pharmacology

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“…Consequently, the rate-limiting step in metabolism of PEA in lung is deamination by MAO (Ben-Harari & Bakhle, 1980). It is possible that PEA enters lung cells without a carrier since in distinction to many other biogenic amines, it crosses the blood-brain barrier (Nakajima, Kakimoto & Sano, 1974) and the uptake process for PEA in lung includes a large diffusion component (Ben-Harari & Bakhle, 1980). The pronounced selectivity of the uptake system in lung (Alabaster, 1977) bestows on the lung the rare ability to control the inactivation of different monoamines.…”

Section: Introductionmentioning

confidence: 99%

Rate‐limiting Step in the Metabolism of Polar and Non‐polar Monoamines in Lung and Liver

Ben-Harari

Peleg

Youdim

1982

British J Pharmacology

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1 Uptake of the non-hydroxylated amines, [14C]-tryptamine and ['4C]-benzylamine in rat lung, infused through the pulmonary circulation, was not saturable over the concentration range 2.5-1,000 AM.2 The kinetic constants for deamination of a variety of hydroxylated and non-hydroxylated monoamines in liver, perfused via the portal circulation, with monoamine oxidase activity in homogenates of liver were similar.3 In lung, uptake of both [14C]-tryptamine and [(4C]-benzylamine was inhibited by the monoamine oxidase inhibitor deprenyl and competition occurred between tryptamine, benzylamine and fphenylethylamine for uptake. 4 These results indicate that tryptamine and benzylamine metabolism in lung is not limited by uptake, unlike that of the hydoxylated amines 5-hydroxytryptamine and noradrenaline and that uptake resembles that of P-phenylethylamine in lung.5 The selectivity of the lung in handling monoamines is not shown by the liver, suggesting that lung has a specific role in clearing certain biogenic monoamines.

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