Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii

Tian, Shengke; Xie, Ruohan; Wang, Haixin; Hu, Yan; Hou, Dandi; Liao, Xingcheng; Brown, Patrick H.; Yang, Hongxia; Lin, Xianyong; Labavitch, John M.; Lu, Lingli

doi:10.1093/jxb/erx112

Cited by 77 publications

(26 citation statements)

References 43 publications

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“…Inside the cells, toxic HMs are often transported to subcellular organelles such as vacuoles and the Golgi apparatus, where the HMs can also be sequestered (Peiter et al, ; Sharma, Dietz, & Mimura, ). For instance, Cd is mainly sequestered in vacuoles of parenchyma cells in the leaf mesophyll, stem pith, and cortex in the shoots of the Zn/Cd hyperaccumulator S. alfredii (Tian et al, ). Vacuolar sequestration of HMs can reduce concentrations of HM ions in the cytosol and thereby alleviate HM toxicity to enzymes involved in cytosolic biochemical reactions.…”

Section: Physiological and Molecular Mechanisms Of Hm Accumulation Inmentioning

confidence: 99%

“…Inside the cells, toxic HMs are often transported to subcellular organelles such as vacuoles and the Golgi apparatus, where the HMs can also be sequestered (Peiter et al, 2007;Sharma, Dietz, & Mimura, 2016). For instance, Cd is mainly sequestered in vacuoles of parenchyma cells in the leaf mesophyll, stem pith, and cortex in the shoots of the Zn/Cd hyperaccumulator S. alfredii (Tian et al, 2017) identifying and functionally characterizing the transporters for these essential metal ions has helped to elucidate the transporters involved in the uptake and translocation of nonessential and toxic HM ions. The roles of many transporters in HM absorption and transport have been characterized in model plants and some hyperaccumulators (Migeon et al, 2010;Shao et al, 2017).…”

Section: Lópezmentioning

confidence: 99%

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Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants

Shi

Zhang

Chen

et al. 2018

Plant Cell & Environment

138

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Uptake, translocation, detoxification, and sequestration of heavy metals (HMs) are key processes in plants to deal with excess amounts of HM. Under natural conditions, plant roots often establish ecto‐ and/or arbuscular‐mycorrhizae with their fungal partners, thereby altering HM accumulation in host plants. This review considers the progress in understanding the physiological and molecular mechanisms involved in HM accumulation in nonmycorrhizal versus mycorrhizal plants. In nonmycorrhizal plants, HM ions in the cells can be detoxified with the aid of several chelators. Furthermore, HMs can be sequestered in cell walls, vacuoles, and the Golgi apparatus of plants. The uptake and translocation of HMs are mediated by members of ZIPs, NRAMPs, and HMAs, and HM detoxification and sequestration are mainly modulated by members of ABCs and MTPs in nonmycorrhizal plants. Mycorrhizal‐induced changes in HM accumulation in plants are mainly due to HM sequestration by fungal partners and improvements in the nutritional and antioxidative status of host plants. Furthermore, mycorrhizal fungi can trigger the differential expression of genes involved in HM accumulation in both partners. Understanding the molecular mechanisms that underlie HM accumulation in mycorrhizal plants is crucial for the utilization of fungi and their host plants to remediate HM‐contaminated soils.

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Section: Physiological and Molecular Mechanisms Of Hm Accumulation Inmentioning

confidence: 99%

Section: Lópezmentioning

confidence: 99%

Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants

Shi

Zhang

Chen

et al. 2018

Plant Cell & Environment

138

View full text Add to dashboard Cite

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“…Recently, the allied species Sedum plumbizincicola was described (Wu et al, 2013). The physiological mechanisms underlying this species' resistance and hyperaccumulation of different metal ions have been investigated (Lu et al, 2008;Jin et al, 2008;Li et al, 2016;Tian et al, 2016;Tian et al, 2017). Analyses of the transcriptome, small RNAs, degradome and proteome have been carried out under Cd stress (Li et al, 2016;Han et al, 2016;.…”

Section: Introductionmentioning

confidence: 99%

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Chen

et al. 2020

Front. Plant Sci.

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The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from Sedum alfredii. Designated as SaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of SaHsfA4c in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript InSaHsfA4c which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of SaHsfA4c under Cd stress was characterized in transgenic Arabidopsis and nonhyperaccumulation ecotype S. alfredii. SaHsfA4c was able to rescue the Cd sensitivity of the Arabidopsis athsfa4c mutant. SaHsfA4c reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and Hsps in transgenic lines. The present results suggest that SaHsfA4c increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of Hsps.

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“…Thus, it is a promising candidate plant species to alleviate and solve soil pollution problems (Clemens et al 2013). There have been many reports on Cd absorption and dynamic balance in S. alfredii (Liu et al 2016;Tian et al 2017); however, the molecular mechanism underlying Cd detoxification in S. alfredii remains poorly understood. GELP family members have been reported in many plant species, and have many roles, including in abiotic stress responses and defense functions (Abdelkafi et al 2009;Cao et al 2018;Dong et al 2016;Lai et al 2017;Tan et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The hyperaccumulating ecotype S. alfredii Hance, with a high tolerance to Zn, Cd and Pb, can grow normally in soil having Cd concentrations up to 400 mg•kg −1 (Tian et al 2017;Xing et al 2013). Leaf vacuolar isolation is currently considered to be the main mechanism of Cd detoxification in hyperaccumulator plants (Rascio & Navari-Izzo 2011;Sharma et al 2016).…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #2 of "Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization of SaGLIP8 in Sedum alfredii Hance under cadmium stress (v0.1)"

2019

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The herb Sedum alfredii (S. alfredii) Hance is a hyper-accumulator of heavy metals [cadmium (Cd), zinc (Zn) and lead (Pb)]; therefore, it could be a candidate plant for efficient phytoremediation. The GDSL esterase/lipase protein (GELP) family plays important roles in plant defense and growth. Although the GELP family members in a variety of plants have been cloned and analyzed, there are limited studies on the family's responses to heavy metal-stress conditions. Methods: Multiple sequence alignments and phylogenetic analyses were performed according to the criteria described. A WGCNA was used to construct co-expression regulatory networks. The roots of S. alfredii seedlings were treated with 100 µM CdCl 2 for qRT-PCR to analyze expression levels in different tissues. SaGLIP8 was transformed into the Cd sensitive mutant strain yeast Δycf1 to investigate its role in resistance and accumulation to Cd. Results: We analyzed GELP family members from genomic data of S. alfredii. A phylogenetic tree divided the 80 identified family members into three clades. The promoters of the 80 genes contained certain elements related to abiotic stress, such as TC-rich repeats (defense and stress responsiveness), heat shock elements (heat stress) and MYB-binding sites (drought-inducibility). In addition, 66 members had tissue-specific expression patterns and significant responses to Cd stress. In total, 13 hub genes were obtained, based on an existing S. alfredii transcriptome database, that control 459 edge genes, which were classified into five classes of functions in a co-expression subnetwork: cell wall and defense function, lipid and esterase, stress and tolerance, transport and transcription factor activity. Among the hub genes, Sa13F.102 (SaGLIP8), with a high expression level in all tissues, could increase Cd tolerance and accumulation in yeast when overexressed. Conclusion: Based on genomic data of S. alfredii, we conducted phylogenetic analyses, as well as conserved domain, motif and expression profiling of the GELP family under Cd-stress conditions. SaGLIP8 could increase Cd tolerance and accumulation in yeast. These results indicated the roles of GELPs in plant responses to heavy metal exposure and provides a theoretical basis for further studies of the SaGELP family's functions.

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Uptake, sequestration and tolerance of cadmium at cellular levels in the hyperaccumulator plant species Sedum alfredii

Abstract: Efficient vacuolar storage of Cd in parenchyma cells, as opposed to its rapid uptake, represents a pivotal process in Cd homeostasis and accumulation in shoots of the Cd hyperaccumulator Sedum alfredii.

Cited by 77 publications

References 43 publications

Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants

Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Peer Review #2 of "Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization of SaGLIP8 in Sedum alfredii Hance under cadmium stress (v0.1)"

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