Uranium biomineralization by a metal resistant Pseudomonas aeruginosa strain isolated from contaminated mine waste

Choudhary, Sangeeta; Sar, Pinaki

doi:10.1016/j.jhazmat.2010.11.004

Cited by 151 publications

(67 citation statements)

References 34 publications

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“…The TEM and XPS results suggested that the cells had the ability to accumulate heavy metals, and that their detoxicity mechanism may be intracellular metal reduction. The peripheral deposition of Ni within the cells conformed to reports on P. aeruginosa strains, which showed the sequestration of intracellular Ni or U in and around the cell periphery (Choudhary and Sar 2010;Sar et al 2001). Such localized metal cation deposition in the cell envelope is attributed to the phosphoryl and carboxyl moieties in the cell wall as well as to membrane components (McLean et al 1996).…”

Section: Discussionsupporting

confidence: 86%

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Zhan

Zhang

2012

Appl Microbiol Biotechnol

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Although microorganisms have the potential to reduce metals, products with elementary forms are unusual. In the present study, a strain of Pseudomonas sp. MBR was tested for its ability to reduce metal ions to their elementary forms coupled to biomineralization under aerobic conditions. The Pseudomonas sp. MBR strain was able to reduce metals such as Fe(III), Mn(II), Cu(II), Ni(II), Cd(II), Co(II), Al(III), Se(IV), and Te(IV) as electron acceptors to elementary forms using citrate, lactate, pyruvate, succinate, malate, glucose, or ethanol as electron donors. Growth and reduction during biomineralization occurred within the pH range of 6.0 to 11.0 and temperature range of 4 to 40°C, with an optimum growth temperature of 28°C. The resistance of Ni (II) varied from 0.5 to 5 mM. Ni(II) reduction was still observed when nitrate was present in addition to oxygen as a potential electron acceptor. The Ni(II) reduction efficiency was related with the molar ratio of the electron donor to Ni(II). Unlike other dissimilatory metal-reducing bacteria, which oxidizes organic matter with Fe(III) or Mn (IV) as the sole electron acceptor coupled to energy production under facultative anaerobic conditions, this strain used oxygen as an electron acceptor combined with metal reduction. The aerobic metal reduction may relate to a co-metabolic reduction. Transmission electron microscopy images demonstrated that the cells had the ability to accumulate heavy metals, and that the detoxicity mechanism was intracellular metal reduction. These results suggested that the use of Pseudomonas sp. MBR could be promising for toxic heavy metal bioremediation and biological metallurgy.

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Section: Discussionsupporting

confidence: 86%

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Zhan

Zhang

2012

Appl Microbiol Biotechnol

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“…A metal-resistant bacterial strain, Pseudomoas aeruginosa J007, isolated from a uranium mine of Uranium Corporation of India Ltd., Jaduguda, India, showed U resistance and accumulation (maximum of 275 mg U/g cell DW) following incubation in 100 mg U/L, at pH 4.0, for 6 h. Microcosm data confirmed that the strain could remove 99 % soluble uranium and sequester it as U oxide and phosphate minerals while maintaining its viability (Choudhary and Sar 2011). Achal et al (2012a) evaluated microbially induced calcite precipitation (MICP) for its potential to remediate strontium (Sr)-contaminated aquifer quartz sand induced by Sr resistant Halomonas spp.…”

Section: Bioremediation Of Multi-metal Waste Sites/radionuclidesmentioning

confidence: 87%

“…However, further characterization and critical biosafety assessment of different contaminated ecosystems such as sewage sludge, nuclear waste, land mines, surface wastewater/sub-surface groundwater and agricultural soils must be taken into account (Achal et al 2011;Choudhary and Sar 2011;Nouri et al 2008;Rajaganapathy et al 2011;Robinson et al 2011;Wang et al 2012). At the same time, genetic pollution of the aforesaid ecosystem through transgenic bacteria must not be ignored .…”

Section: Introductionmentioning

confidence: 99%

Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

Mani

Kumar

2013

Int. J. Environ. Sci. Technol.

517

137

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The ability of heavy metals bioaccumulation to cause toxicity in biological systems-human, animals, microorganisms and plants-is an important issue for environmental health and safety. Recent biotechnological approaches for bioremediation include biomineralization (mineral synthesis by living organisms or biomaterials), biosorption (dead microbial and renewable agricultural biomass), phytostabilization (immobilization in plant roots), hyperaccumulation (exceptional metal concentration in plant shoots), dendroremediation (growing trees in polluted soils), biostimulation (stimulating living microbial population), rhizoremediation (plant and microbe), mycoremediation (stimulating living fungi/mycelial ultrafiltration), cyanoremediation (stimulating algal mass for remediation) and genoremediation (stimulating gene for remediation process). The adequate restoration of the environment requires cooperation, integration and assimilation of such biotechnological advances along with traditional and ethical wisdom to unravel the mystery of nature in the emerging field of bioremediation. This review highlights better understanding of the problems associated with the toxicity of heavy metals to the contaminated ecosystems and their viable, sustainable and eco-friendly bioremediation technologies, especially the mechanisms of phytoremediation of heavy metals along with some case studies in India and abroad. However, the challenges (biosafety assessment and genetic pollution) involved in adopting the new initiatives for cleaning-up the heavy metals-contaminated ecosystems from both ecological and greener point of view must not be ignored.

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“…Such observations strongly suggest that Co 2+ interacted with phosphate groups in R. palustris to form cobalt phosphate octahydrate crystals. In a similar fashion, P. aeruginosa accumulated uranium due to precipitation of intracellular crystalline U-phosphate compounds [35]. The formation of nickel phosphides and carbides was also reported, primarily in the cell envelope of P. aeruginosa, indicating the predominant role of the phosphoryl and carboxyl/carbonyl groups in cell wall/ membrane components for metal sequestration [34].…”

Section: X-ray Diffraction Analysismentioning

confidence: 81%

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

Gao

Wang

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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Bioaccumulation by growing cells is a potential technique for the removal of heavy metals from wastewater. Cobalt bioaccumulation by Rhodopseudomonas palustris via different metabolic pathways was investigated for various pH levels, temperatures, co-cations and initial Co 2+ concentrations. The distribution of cobalt and its chemical nature in R. palustris were examined by cell compartmentalization and X-ray diffraction analysis. Our results indicated that biomass and bioaccumulation capacity were superior under aerobic conditions in the dark at pH 6.5 and 30 C. Cobalt removal efficiency and bioaccumulation capacity were, respectively, 84. 9% and 287.18 . Cobalt was distributed between the cell surface, periplasmic space, membrane and cytoplasm. The periplasmic space and membrane accounted for 57.37% and 27.95% of Co 2+ uptake, respectively. X-ray diffraction analysis showed that cobalt ions were predominantly deposited in the cell as cobalt phosphate octahydrate. These results demonstrate that R. palustris could be used to remove cobalt from industrial wastewater; it would therefore be beneficial to further study the mechanism of cobalt bioaccumulation in this organism.

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Uranium biomineralization by a metal resistant Pseudomonas aeruginosa strain isolated from contaminated mine waste

Cited by 151 publications

References 34 publications

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

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