Background: Inductively coupled plasma mass spectrometry (ICP-MS) techniques have been widely used for analysis of long-lived environmental radionuclides. In this paper, we present an optimization of the sector field (SF)-ICP-MS technique for the analysis of 226 Ra in groundwater samples using a method of pre-concentration of radium in water samples. Methods: The separation protocol and a sequential application of ion exchange and extraction chromatography have been optimized, and related polyatomic interferences and matrix effects affecting the