Urban air pollution induces redox imbalance and epithelium hyperplasia in mice cornea

Vitar, Romina Mayra Lasagni; Arana, Ailen Gala Hvozda; Janezic, Natasha S.; Marchini, Timoteo; Tau, Julia; Martinefski, Manuela; A, Tesone; Racca, Lourdes; Reides, Claudia; Trípodi, Valeria; Evelson, Pablo; Berra, Alejandro; Llesuy, Susana; Ferreira, Sandra M.

doi:10.1016/j.taap.2019.114770

Cited by 22 publications

(7 citation statements)

References 55 publications

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“…On the other hand, our previous work demonstrated that our mouse model of air pollution exposure showed increased oxidative stress in corneal epithelial cells (11), potentially enhancing the susceptibility to viral infection and replication despite the increased antiviral mediator production like interferons (52).…”

mentioning

confidence: 76%

“…Two air forced ventilated chamber systems located in a highly populated area of Buenos Aires City (latitude 34°35'51''S and longitude 58°23'56'O) were used as previously described (11). One chamber received urban airflow from Buenos Aires city and the other one received filtered indoor airflow.…”

Section: Polluted Air Chronic Exposure Mouse Modelmentioning

confidence: 99%

“…Traffic emissions make an important contribution to the PM2.5 composition in Buenos Aires and other big cities' polluted air, causing adverse biological responses in vivo and in vitro (6)(7)(8). Our group and others have demonstrated in vitro that corneal and conjunctival epithelial cells increase the secretion of pro-inflammatory cytokines with redox imbalance under incubation with diesel exhaust particles (DEP) (the main component of urban PM); moreover, in vivo exposure to air pollution from Buenos Aires altered the ocular surface cellularity, redox imbalance and the inflammatory cytokines on the cornea (9)(10)(11). On the other hand, three studies have shown that air pollution increases the susceptibility of respiratory mucosa to the H1N1 influenza (12,13) virus and respiratory syncytial virus (RSV) (14).…”

Section: Introductionmentioning

confidence: 99%

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Polluted Air Exposure Compromises Corneal Immunity and Exacerbates Inflammation in Acute Herpes Simplex Keratitis

et al. 2021

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Air pollution is a serious environmental issue worldwide in developing countries’ megacities, affecting the population’s health, including the ocular surface, by predisposing or exacerbating other ocular diseases. Herpes simplex keratitis (HSK) is caused by the herpes simplex virus type 1 (HSV-1). The primary or recurring infection in the ocular site causes progressive corneal scarring that may result in visual impairment. The present study was designed to study the immunopathological changes of acute HSK under urban polluted air, using the acute HSK model combined with an experimental urban polluted air exposure from Buenos Aires City. We evaluated the corneal clinical outcomes, viral DNA and pro-inflammatory cytokines by RT-PCR and ELISA assays, respectively. Then, we determined the innate and adaptive immune responses in both cornea and local lymph nodes after HSV-1 corneal by immunofluorescence staining and flow cytometry. Our results showed that mice exposed to polluted air develop a severe form of HSK with increased corneal opacity, neovascularization, HSV-1 DNA and production of TNF-α, IL-1β, IFN-γ, and CCL2. A high number of corneal resident immune cells, including activated dendritic cells, was observed in mice exposed to polluted air; with a further significant influx of bone marrow-derived cells including GR1+ cells (neutrophils and inflammatory monocytes), CD11c+ cells (dendritic cells), and CD3+ (T cells) during acute corneal HSK. Moreover, mice exposed to polluted air showed a predominant Th1 type T cell response over Tregs in local lymph nodes during acute HSK with decreased corneal Tregs. These findings provide strong evidence that urban polluted air might trigger a local imbalance of innate and adaptive immune responses that exacerbate HSK severity. Taking this study into account, urban air pollution should be considered a key factor in developing ocular inflammatory diseases.

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mentioning

confidence: 76%

Section: Polluted Air Chronic Exposure Mouse Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polluted Air Exposure Compromises Corneal Immunity and Exacerbates Inflammation in Acute Herpes Simplex Keratitis

et al. 2021

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“…In addition, ROS production induce a redox imbalance sufficient to initiate nuclear factor erythroid 2-related factor 2 (Nrf2) signaling that translocates to the nucleus to enhance the cellular antioxidant capacity ( 123 ). Interestingly, whole-body exposure to urban air models in mice showed similar results with macromolecular oxidative damage due to redox imbalance along with an inflammatory response modulated by the increase in IL-10 levels after 1 week of exposure, which early regulates the release of TNF-α and IL-6 ( 124 ).…”

Section: Mitochondrial Function In Inflammatory and Metabolic Diseasementioning

confidence: 91%

“…For example, it has been shown that PM exacerbates irritation, burning, foreign body sensation, redness, itching in the eyes of people living in urban areas (116)(117)(118)(119)(120)(121). Detrimental PM effects on ocular surface are associated with oxidative stress and proinflammatory pathways (122)(123)(124)(125)(126). In this sense, both corneal and conjunctival epithelial cells exposed to different PM surrogates in vitro increase the inflammatory mediators' production (127-130).…”

Section: Pm Detrimental Effects On the Ocular Surfacementioning

confidence: 99%