Urban Development in the Margins of a World Heritage Site

Esposito, Adèle

doi:10.1515/9789048534050

Cited by 7 publications

(10 citation statements)

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“…One challenge, however, is that the application of fluorescence lifetime sensing in biology turned its focus toward fluorescence lifetime imaging microscopy (FLIM) early on 32,33 . FLIM offers great spatial resolution and molecular specificity for a number of analytes 34,35 including protons 28,[36][37][38] , but in practice it is not yet a widely accessible technique because of the requirement for expensive commercial or custom-built systems. However, in many studies, high spatial resolution is not required to obtain critical understanding of live-cell physiology, and population measurements are entirely sufficient.…”

Section: Graphical Abstractmentioning

confidence: 99%

Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor

et al. 2019

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Intracellular pH plays a key role in physiology, and its measurement in living specimens remains a crucial task in biology. Fluorescent protein-based pH sensors have gained widespread use, but there is limited spectral diversity for multicolor detection, and it remains a challenge to measure absolute pH values. Here we demonstrate that mCherryTYG is an excellent fluorescence lifetime pH sensor that significantly expands the modalities available for pH quantification in live cells. We first report the 1.09 Å X-ray crystal structure of mCherryTYG, exhibiting a fully matured chromophore. We next determine that it has an extraordinarily large dynamic range with a 2 nanosecond lifetime change from pH 5.5 to pH 9.0. Critically, we find that the sensor maintains a pKa of 6.8 independent of environment, whether as the purified protein in solution or expressed in live cells. Furthermore, the lifetime measurements are ro-bustly independent of total fluorescence intensity and scatter. We demonstrate that mCherryTYG is a highly effective sensor using time resolved fluorescence spectroscopy on live-cell suspensions, which has been previously overlooked as an easily accessible approach for quantifying intracellular pH. As a red fluorescent *

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Section: Graphical Abstractmentioning

confidence: 99%

Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor

et al. 2019

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“…This Committee has become an arena of negotiation and struggle about doctrines of conservation and development (Peycam, 2016). At the same time, UNESCO's listing of Angkor repositioned the country on the expanding routes of international tourism (Winter, 2007), which has become one of the pillars of national economic growth (Esposito, 2018a;Esposito and Fauveaud, 2018).…”

Section: How State Disengagement Generates Heritage 'Inbetweenness'mentioning

confidence: 99%

Beyond official heritage agendas: The third space of conservation practices in Phnom Penh, Cambodia

Fauveaud

Esposito²

2020

Urban Studies

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Urban heritage conservation has often been portrayed as a practice shaped by ‘authorised discourses’ which are produced by powerful actors including the state, international organisations and experts. But the literature has also paid attention to non-governmental actors who produce ‘unauthorised heritage discourses’ by calling for broader and more diversified heritage interpretations and practices. Using the case of Phnom Penh, the capital of Cambodia, we argue that the dichotomy between authorised and unauthorised heritage has produced artificial boundaries between those legacies which have been (or should be) identified as heritage and the multiple remains of the past which nobody has ever attempted to define as such. Instead, we argue that multiple authorised discourses, circulating worldwide, generate a pervasive global hierarchy of value which relates to heritage. Various actors, including bilateral donors, states’ representatives, tycoons, owners and tenants, shape urban tactics which selectively appropriate components of this hierarchy and combine them with socio-political and economic rationalities in order to conserve Phnom Penh’s urban legacies. Taken together, these tactics shape what we name a ‘third space of heritage hybridity’ outside the scope of official agendas.

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“…Research on Phnom Penh's vanishing urban heritage remains scarce. Scholars have typically focused on the politics of heritage at Angkor, the archaeological site of the ancient Khmer capitals between the 9th-and 15th-centuries, which was listed as a UNESCO World Heritage Site in 1992 (Esposito, 2018b;Miura, 2011;Winter, 2008). Literature and expert reports on Phnom Penh mainly deal with the development of a new metropolis (Percival and Waley, 2012;Shatkin, 1998), environmental issues (Biswas and Tortajada, 2010), the politics of land (Carrier, 2007), social justice (Simone, 2008), and contemporary real estate development (Fauveaud, 2015).…”

Section: Reframing Phnom Penh's Heritage Politics In the International Scenementioning

confidence: 99%

“…They adopted legislation with the primary objective of safeguarding Angkor. For the same reason, Cambodian institutions willingly endorsed the transfer of international knowledge about archaeological conservation and management planning that was applied in the international program for safeguarding Angkor (Esposito, 2018b), but were resistant to the importation of international knowledge about urban heritage.…”

Section: Three Sources Of Atomizationmentioning

confidence: 99%

The atomization of heritage politics in post-colonial cities: The case of Phnom Penh, Cambodia

Esposito¹,

Fauveaud

2018

Environment and Planning C: Politics and Space

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This paper analyzes the politics of heritage in urban Cambodia. Focusing on the capital, Phnom Penh, we argue that urban heritage is shaped at the intersection of global doctrines and professional knowledge, socioeconomic strategies at the national and local scales, real estate developments, and contextual institutional practices. We propose the concept “atomization of heritage politics” to explain the fragmentary and tentacular power relations that determine how built heritage is managed or destroyed. Drawing on heritage literature on Southeast Asian cities, we first argue that academic writers underestimate the role of land issues and localized power relations in shaping urban heritage politics. We contextualize the rise of heritage concerns in Cambodia. Since colonial times heritage strategies have focused on the UNESCO World Heritage Site of Angkor, with urban heritage being of only peripheral concern. Second, we explain how proposals made by international development partners for an overall heritage policy failed, as they conflicted with the rationality of land and power relations, leaving foreign consultants to renegotiate their position in Cambodian politics. Finally, we explore two case studies—the National Stadium and the Renakse Hotel—at the center of virulent, long-lasting political clashes between various forces that took root during the reconstruction of the Cambodian state in the 1980s and 1990s. Together with powerful individuals, families, and companies, the competition and tactical alliances between these forces shape the contested politics of urban heritage in the Cambodian capital.

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Urban Development in the Margins of a World Heritage Site

Cited by 7 publications

References 0 publications

Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor

Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor

Beyond official heritage agendas: The third space of conservation practices in Phnom Penh, Cambodia

The atomization of heritage politics in post-colonial cities: The case of Phnom Penh, Cambodia

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