Urban stream microbial communities show resistance to pharmaceutical exposure

Bechtold, Heather A.; Snow, Daniel D.; Rojas, M. Guadalupe; Reisinger, Alexander J.; Kelly, John J.

doi:10.1002/ecs2.2041

Cited by 50 publications

(39 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…magna . Our approach of using low concentrations of contaminants to match their normal occurrence in the environment fits in with the latest research [ 16 , 17 ]. This kind of study go beyond the mere assessment of the impacts of contaminants on mortality and reproduction and contribute to our understanding of the sub-lethal effects of anthropogenic chemicals at the cellular and molecular levels.…”

Section: Discussionmentioning

confidence: 64%

“…Recently concentrations of pharmaceuticals as present in freshwater ecosystems have come into focus. Medicine have been shown to significantly affect the functioning of aquatic biofilms [ 16 ]. Environmental concentrations of pharmaceuticals can also significantly affect primary producers (algae) crossing two trophic planktonic levels, affecting Daphnia [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

How cyclophosphamide at environmentally relevant concentration influences Daphnia magna life history and its proteome

et al. 2018

View full text Add to dashboard Cite

The waste of commonly used medicines is known to contaminate freshwater ecosystems. Pharmaceuticals can be toxic, mutagenic, or modifying to freshwater organisms even at low concentrations if consider their permanent presence in the environment. Chemotherapeutics used to treat cancer, and in particular alkylating agents, contribute significantly to this form of pollution, the latter introducing cytotoxic and/or mutagenic lesions to the DNA and RNA of organisms which can be disruptive to their cells. The aim of the present study was to investigate the influence of the alkylating anticancer agent cyclophosphamide (CP) on Daphnia magna clones. We evaluated the life history parameters and protein profiles of this crustacean following exposure to environmentally relevant CP concentration of 10 ng L-1. Even at this low concentration, the alkylating agent caused modification of the life history parameters and proteome profile of the Daphnia. These changes were clone-specific and involved growth rate, age at first reproduction, neonate number, and proteins related to cell cycle and redox state regulation. The disturbance caused by pharmaceuticals contaminating freshwater ecosystem is probably weaker and unlikely to be cytotoxic in character due to the high dilution of these substances in the water. However, our results indicate that prolonged exposure of organisms to these toxins may lead to modifications on the organismal and molecular levels with unpredictable significance for the entire ecosystem.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

How cyclophosphamide at environmentally relevant concentration influences Daphnia magna life history and its proteome

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Exposure to PPCPs from wastewater effluent is a pressing concern for natural resource managers and freshwater scientists (Rosi-Marshall & Royer, 2012;Bernhardt et al, 2017). Work examining PPCPs has demonstrated they are pervasive in wastewater (Brausch & Rand, 2011;Corcoll et al, 2015), and have the potential to affect aquatic communities and alter ecosystem processes (Rosi et al, 2018). Local authorities are particularly concerned about the potential environmental and human-health consequences of PPCPs, as they often persist through conventional drinking water treatment (Wang & Wang, 2016) and have the potential to negatively affect human populations.…”

Section: Ecological Implications Of Wastewater Inputs In Freshwater Smentioning

confidence: 99%

Wastewater infrastructure and the ecology and management of freshwater systems

Capps

2019

Acta Limnol. Bras.

View full text Add to dashboard Cite

Globally, freshwater resources are influenced by inputs of energy, nutrients, and pollutants from human wastewater. Local resource managers and policy-makers are tasked to address ecological and human-health concerns associated with aging and obsolete water infrastructure using limited financial resources. Nevertheless, there is limited information available describing how waste streams vary in their pollutant load or their subsequent effects on ecosystem structure and function in streams and rivers. Consequently, as wastewater systems degrade, local resource managers and policy makers are forced to develop watershed management strategies to deal with increasing effluent discharge without an understanding of how their decisions will influence local ecological processes or the structural and functional integrity of downstream habitats. Here, I discuss some of the ecological implications of obsolete or absent wastewater treatment, and describe how mismatches between the governance of wastewater management and watershed ecology may exacerbate environmental problems.

show abstract

“…Like nutrients, pharmaceuticals are ubiquitous in human impacted surface waters ( Kolpin et al, 2002 ; Pal et al, 2010 ) and can alter biofilm bacterial biomass and composition ( Lawrence et al, 2005 ; Rosi-Marshall et al, 2013 ; Rosi et al, 2018 ). Pharmaceutical pollution is also a global issue: pharmaceuticals or their transformation products are at least detectable in many aquatic ecosystems of seventy-one countries on all continents ( aus der Beek et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Nutrients and Pharmaceuticals Structure Bacterial Core Communities in Urban and Montane Stream Biofilms

et al. 2020

View full text Add to dashboard Cite

Bacteria in stream biofilms contribute to stream biogeochemical processes and are potentially sensitive to the substantial levels of pollution entering urban streams. To examine the effects of contaminants on stream biofilm bacteria in situ , we exposed growing biofilms to experimental additions of nutrients [nitrogen (N), phosphorus (P), and iron (Fe)], pharmaceuticals (caffeine and diphenhydramine), nutrients plus pharmaceuticals, or no contaminants using contaminant exposure substrates (CES) in three catchments in northern Utah. We performed our study at montane and urban sites to examine the influence of existing pollution on biofilm response. We identified bacterial core communities (core) for each contaminant treatment at each land-use type (e.g., nutrient addition montane bacterial core, nutrient addition urban bacterial core, pharmaceutical addition montane bacterial core) by selecting all taxa found in at least 75% of the samples belonging to each specific grouping. Montane and urban land-use distinguished bacterial cores, while nutrients and pharmaceuticals had subtle, but nonetheless distinct effects. Nutrients enhanced the dominance of already abundant copiotrophs [i.e., Pseudomonadaceae (Gammaproteobacteria) and Comamonadaceae (Betaproteobacteria)] within bacterial cores at montane and urban sites. In contrast, pharmaceuticals fostered species-rich bacterial cores containing unique contaminant-degrading taxa within Pseudomonadaceae and Anaerolineaceae (Chloroflexi). Surprisingly, even at urban sites containing ambient pharmaceutical pollution, pharmaceutical additions increased bacterial core richness, specifically within DR-16 (Betaproteobacteria), WCHB1-32 (Bacteroidetes), and Leptotrichiaceae (Fusobacteria). Nutrients exerted greater selective force than pharmaceuticals in nutrient plus pharmaceutical addition treatments, creating bacterial cores more closely resembling those under nutrient rather than pharmaceutical addition, and promoting unique Oscillatoriales (Cyanobacteria) taxa in urban streams. Our results show that additions of N, P, and Fe intensified the dominance of already abundant copiotrophs, while additions of caffeine and diphenhydramine enabled unique taxa associated with contaminant degradation to participate in bacterial cores. Further, biofilm bacteria at urban sites remained sensitive to pharmaceuticals commonly present in waters, suggesting a dynamic interplay among pharmaceutical pollution, bacterial diversity, and contaminant degradation.

show abstract

Urban stream microbial communities show resistance to pharmaceutical exposure

Cited by 50 publications

References 71 publications

How cyclophosphamide at environmentally relevant concentration influences Daphnia magna life history and its proteome

How cyclophosphamide at environmentally relevant concentration influences Daphnia magna life history and its proteome

Wastewater infrastructure and the ecology and management of freshwater systems

Nutrients and Pharmaceuticals Structure Bacterial Core Communities in Urban and Montane Stream Biofilms

Contact Info

Product

Resources

About