Urchin peroxidase-mimicking Au@Pt nanoparticles as a label in lateral flow immunoassay: impact of nanoparticle composition on detection limit of Clavibacter michiganensis

Panferov, Vasily G.; Safenkova, Irina V.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1007/s00604-020-04253-3

Cited by 34 publications

(22 citation statements)

References 30 publications

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“…The SA value of Au@Ag-Pt NPs was about 20 times higher than for Au@Pt nanoparticles synthesized by the reduction of Na2PtCl6 on Au NPs. 31 The SA value of Au@Ag #2-Pt #4 NPs was about 80% compared to Au@Ag #2-Pt #1-4 NPs.…”

Section: Peroxidase-mimicking Activity Of Nanoparticlesmentioning

confidence: 93%

Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

Panferov

Byzova²,

Zherdev³

et al. 2021

Preprint

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We report a new approach to synthesize nanozyme with low consumption of precious Pt-precursor and high peroxidase-mimicking activity. The synthesis includes the formation of gold nanoparticles (Au NPs), the overgrowth of a silver layer over Au NPs (Au@Ag NPs), and the galvanic replacement of Ag with PtCl<sub>6</sub><sup>2-</sup> leading to the formation of Au@Ag–Pt NPs with uniformly deposited catalytic Pt sites. The reported approach facilitates up to 20-times lower consumption of Pt precursor. The use of Au@Ag-Pt NPs as the catalytic label in lateral flow immunoassay results in a 65-fold lower limit of detection (15 pg/mL in serum).

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Section: Peroxidase-mimicking Activity Of Nanoparticlesmentioning

confidence: 93%

Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

Panferov

Byzova²,

Zherdev³

et al. 2021

Preprint

Self Cite

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“…For qualitative detection, the results of ICA strips can be observed by the naked eye. For quantitative detected, the ICA strips were scanned by an HP ScanJet (HP ScanJet g3110, Shanghai, China), and the digital images of strips were analyzed by the TotalLab TL120 software (Nonlinear Dynamics, Newsletter, UK) [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B1 in Maize

Wang

Shen

et al. 2021

Biosensors

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Aflatoxin B1 (AFB1), a mycotoxin, is hepatotoxic, carcinogenic, and nephrotoxic in humans and animals, and contaminate a wide range of maize. In this study, an immunochromatographic assay (ICA) based on polystyrene microspheres (PMs) was developed for sensitive and quantitative detection of AFB1 in maize. The amounts of PMs, the condition for activating carboxyl groups of PMs, the amount of monoclonal antibody (mAb), and the volume of the immune probe were optimized to enhance the performance PMs-ICA for point-of-care testing of AFB1 in maize. The PMs-ICA showed the cut-off value of 1 ng/mL in phosphate buffer (PB) and 6 µg/kg in maize samples, respectively. The quantitative limit of detection (qLOD) was 0.27 and 1.43 µg/kg in PB and maize samples, respectively. The accuracy and precision of the PMs-ICA were evaluated by analysis of spiked maize samples with recoveries of 96.0% to 107.6% with coefficients of variation below 10%. In addition, the reliability of PMs-ICA was confirmed by the liquid chromatography-tandem mass spectrometry method. The results indicated that the PMs-ICA could be used as a sensitive, simple, rapid point-of-care testing of AFB1 in maize.

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“…Figure 4 summarizes the application data and the various types of nanoparticle modifications described below. [74], Pt-nanoflower-type NPs [75], Au@PTNps [76], Au@Ag-PtNPs [77], quantum dots [78][79][80], magnetic beads [81].…”

Section: Immunodiagnostic Systems Based On Nanomaterialsmentioning

confidence: 99%

“…The authors of [76] investigated the effect of the composition of Au@Pt nanoparticles, their morphology, and peroxidase-like activity on the detection limit of the lateral flow immunoassay. Fourteen types of nanoparticles were synthesized by varying the concentration of Pt 4+ (20-2000 µM) using gold nanoparticles (diameter, 20.0 ± 2.6 nm) as a seed and ascorbic acid as a reducing agent.…”

Section: Immunochromatographic Test Systems (Lfia)mentioning

confidence: 99%

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Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

Rudenko

Fursova

Shepelyakovskaya

et al. 2021

Sensors

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The recognition of biomolecules is crucial in key areas such as the timely diagnosis of somatic and infectious diseases, food quality control, and environmental monitoring. This determines the need to develop highly sensitive display devices based on the achievements of modern science and technology, characterized by high selectivity, high speed, low cost, availability, and small size. Such requirements are met by biosensor systems—devices for reagent-free analysis of compounds that consist of a biologically sensitive element (receptor), a transducer, and a working solution. The diversity of biological material and methods for its immobilization on the surface or in the volume of the transducer and the use of nanotechnologies have led to the appearance of an avalanche-like number of different biosensors, which, depending on the type of biologically sensitive element, can be divided into three groups: enzyme, affinity, and cellular/tissue. Affinity biosensors are one of the rapidly developing areas in immunoassay, where the key point is to register the formation of an antigen–antibody complex. This review analyzes the latest work by Russian researchers concerning the production of molecules used in various immunoassay formats as well as new fundamental scientific data obtained as a result of their use.

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Urchin peroxidase-mimicking Au@Pt nanoparticles as a label in lateral flow immunoassay: impact of nanoparticle composition on detection limit of Clavibacter michiganensis

Cited by 34 publications

References 30 publications

Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

Peroxidase-Mimicking Nanozyme with Surface-Dispersed Pt Atoms as the Label for the Lateral Flow Immunoassay of C-Reactive Protein

Polystyrene Microsphere-Based Immunochromatographic Assay for Detection of Aflatoxin B1 in Maize

Antibodies as Biosensors’ Key Components: State-of-the-Art in Russia 2020–2021

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