Uric acid protects membranes and linolenic acid from ozone-induced oxidation

Meadows, Jan; Smith, Robert C.; Reeves, Jeri

doi:10.1016/0006-291x(86)91243-x

Cited by 19 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Production of uric acid via adenosine nucleotide turnover may occur in every tissue in the body, and uric acid may be increased throughout the body, because the large amount of acetate formed from ethanol in the liver is probably released and utilized at other tissues 43) . Uric acid is considered one of the major antioxidants in plasma that inhibits oxidative damage, such as lipid peroxidation [25][26][27][28] . In our study, the level of 8-OHdG decreased with the level of uric acid, although the total urinary biopyrrin level was not correlated with the 8-OHdG levels.…”

Section: Discussionmentioning

confidence: 99%

“…The level of urinary 8-hydroxydeoxyguanosine (8-OHdG), a useful marker of oxidative DNA damage [20][21][22] , was selected as an indicator for estimating the level of oxidative DNA damage. The serum level of uric acid, which varies with the amount of ethanol consumed 23,24) , was measured as an antioxidant in the serum [25][26][27][28] . Blood samples were collected from an antecubital vein of each subject in the morning.…”

Section: Biological and Hematological Measurementsmentioning

confidence: 99%

See 1 more Smart Citation

Moderate Alcohol Consumption Reduces Urinary 8-Hydroxydeoxyguanosine by Inducing of Uric Acid.

Yoshida

Shioji²,

Kishida

et al. 2001

INDUSTRIAL HEALTH

View full text Add to dashboard Cite

Recent studies suggest that moderate alcohol consumption is associated with a low risk of cancer, coronary heart disease, and other diseases. Most of these diseases are considered to be related to the action of reactive oxygen species (ROS) at certain stages of disease progression. However, considerable evidence exists indicating that ethanol generates ROS in vivo. Thus, the reduced risk of disease as a result of alcohol consumption seems to contradict evidence suggesting the induction of ROS by ethanol. In the present study, we investigated whether oxidative stress was induced in moderate alcohol drinkers. We measured the total urinary biopyrrins and 8-hydroxydeoxyguanosine (8-OHdG) levels as a systemic oxidative stress marker and an oxidative DNA damage marker, respectively. Serum uric acid was also measured as an alcohol-induced antioxidant. We compared total urinary biopyrrins and 8-OHdG levels among groups with different alcohol habits. The results showed that total biopyrrins levels increased with the amount of alcohol consumed, but that the level of 8-OHdG significantly decreased with the amount of alcohol consumed. The decrease in 8-OHdG levels seemed to be associated with increasing levels of uric acid. Judging from the increasing level of total biopyrrins, alcohol may induce ROS. ROS may then cause cell damage in liver, as suggested by the positive correlation between the total biopyrrins levels and the serum GOT, GPT, and γ γ γ γ γ-GTP levels. However, since ROS may be more effectively counteracted by uric acid in organs other than the liver, DNA damage may be surpressed rather than induced. Accordingly, moderate alcohol consumption seems to have the overall effect of reducing DNA damage, as shown by the decrease in urinary 8-OHdG levels observed in our study.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Biological and Hematological Measurementsmentioning

confidence: 99%

Moderate Alcohol Consumption Reduces Urinary 8-Hydroxydeoxyguanosine by Inducing of Uric Acid.

Yoshida

Shioji²,

Kishida

et al. 2001

INDUSTRIAL HEALTH

View full text Add to dashboard Cite

show abstract

“…Note the higher levels of uric acid in the nasal fluids. Since uric acid reacts quickly with 03, it has been suggested that uric acid acts as a "scrubber" of inhaled 03 in the nose, perhaps protecting the deeper parts of the respiratory tract by diminishing the amount of inhaled 03 that is delivered there (4,5,32,56). …”

Section: Oxidant Effects On Plasmamentioning

confidence: 99%

Oxidants, antioxidants, and respiratory tract lining fluids.

Cross

Vliet

O’Neill

et al. 1994

Environ Health Perspect

222

View full text Add to dashboard Cite

Respiratory tract lining fluids (RTLFs) are a heterogeneous group of substances covering the respiratory tract epithelial cells (RTECs) from nasal mucosa to alveoli. Antioxidants contained in the RTLFs can be expected to provide an initial defense against inhaled environmental toxins. The major antioxidants in RTLF include mucin, uric acid, protein (largely albumin), ascorbic acid, and reduced glutathione (GSH). RTLF antioxidants can be augmented by such processes as transudation/exudation of plasma constituents; RTEC secretory processes, including glandular mucus secretion; and cellular antioxidants derived from lysis of RTECs and of inflammatory cells. The antioxidant composition of RTLFs and their role in modulating normal and pathophysiologic RTEC functions under conditions of oxidative stress are yet to be fully characterized. -Environ Health Perspect 102(Suppl 10): 185-191 (1994)

show abstract

“…Most mammals, including humans, reabsorb virtually all filtered urate in the proximal convoluted tubule (98%). Evidence in mammalian systems indicates that uric acid is an efficient free radical scavenger (Becker et al, 1989;Green et al, 1986;Meadows et al, 1986;Becker et al, 1991;Peden et al, 1990), however, the beneficial effects from retention of urate must be carefully balanced by excretion to avoid detrimental effects such as gout (Scott, 1971). In fact, a urate secretory flux is also present and contributes to excretion (Gutman and Yu, 1972) such that, even in mammals, excreted urate may derive primarily from proximal tubule secretion (Gutman and Yu, 1972).…”

Section: Introductionmentioning

confidence: 99%

Transepithelial urate transport by avian renal proximal tubule epithelium in primary culture

Dudas

Pelis

Braun

et al. 2005

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY Birds are uricotelic, and because they excrete urate by renal tubular secretion, they provide a convenient model for examination of this process. Primary monolayer cultures of the isolated renal proximal tubule epithelium from the domestic chicken, Gallus gallus L., were mounted in Ussing chambers where several substrates/inhibitors of renal organic anion transporters were tested for the sidedness and specificity of their effects on transepithelial urate transport. Transepithelial electrical resistance,electrical potential and sodium-dependent glucose current were monitored to detect nonspecific effects. Under control short-circuited conditions the ratio of unidirectional fluxes of [14C]urate was found to be 3:1. Active net secretion was specifically inhibited by 1 mmol l–1probenecid and 10 mmol l–1para-aminohippuric acid(PAH). Bromocresol Green, cimetidine, nocodozole, cytochalasin D and ouabain also inhibited secretion but were toxic. Interstitial-side lithium (5 mmol l–1) and glutarate (1 mmol l–1) specifically blocked transport, but 10–100 μmol l–1 glutarate had no effect. Interstitial estrone sulfate (ES) stimulated urate secretion at 10μmol l–1 but was inhibitory at 500 μmol l–1. Active PAH secretion (5:1 flux ratio) was inhibited 34%by 330 μmol l–1 urate. ES (500 μmol l–1) blocked the remainder. From the lumen side,glucose-free, Cl--free and high K+ (30 mmol l–1) solutions, or an alkaline pH of 7.7 had no effect on urate transport and neither did several compounds known to be uricosuric. Lumen-side methotrexate (500 μmol l–1) and MK571 (20μmol l–1) strongly inhibited urate secretion. MK571 had no effect from the interstitial side. RT-PCR revealed mRNA for OAT1-, OAT3-,MRP2- and MRP4-like organic anion transporters in chicken proximal epithelium.

show abstract

Uric acid protects membranes and linolenic acid from ozone-induced oxidation

Cited by 19 publications

References 22 publications

Moderate Alcohol Consumption Reduces Urinary 8-Hydroxydeoxyguanosine by Inducing of Uric Acid.

Moderate Alcohol Consumption Reduces Urinary 8-Hydroxydeoxyguanosine by Inducing of Uric Acid.

Oxidants, antioxidants, and respiratory tract lining fluids.

Transepithelial urate transport by avian renal proximal tubule epithelium in primary culture

Contact Info

Product

Resources

About