Uridyl Transferases and the Formation of Uridine Triphosphate: Enzymic Production of Uridine Triphosphate: Uridine Diphosphoglucose Pyrophosphorolysis

GAL3 gene product is required for maintenance of the induced state of the GAL cluster genes in Saccharomyces cerevisiae

“…7). UDP-glucose cannot be the actual inducer because it is known to be synthesized by the action of UDP-glucose pyrophosphorylase from glucose 1-phosphate and UTP (16) but not from galactose.…”

Structure and Function of Prokaryotic UDP-Glucose Pyrophosphorylase, A Drug Target Candidate

“…Presence of uridine, phosphate and glucose in this compound, as well as the correct linkage of the different components were ascertained, thus identifying it as UDP-glucose, the first sugar nucleotide to be discovered, and described as a «coenzyme of the galactose phosphate-glucose phosphate transformation» [10]. Later, Kalckar et al detected UDPG:PP activity in yeast samples [11]. Specifically, they found out that dialyzed yeast maceration juice, when in the presence of UDP-glucose, promoted the formation of inorganic pyrophosphate and UTP, hence discovering the reverse reaction.…”

Pyrophosphorylases and Phosphorylases in Biosynthetic Reactions

“…There were a number of reasons for suspecting the existence of kinases that act on the other nucleoside monophosphates. A variety of purine and pyrimidine nucleoside di-and triphosphates had been found in nature (19,163,205); UTP was known to function in the synthesis of uridine coenzymes (110,181); and studies of coenzyme synthesis created an impression that nucleoside 5'-polyphosphates would prove to be precursors of polynucleotides (122). An enzyme was in time found (153) (155) could be distinguished from a specific adenylate kinase in the preparation by the greater heat stability of the latter.…”