Urinary 17-Ketosteroids in Metabolism1

The Urinary Excretion of Neutral 17-Ketosteroids in Childhood

“…In adults, they found, in agreement with Talbot et al [1940] 3(/8)-hydroxy-17 -ketosteroids amounted to less than 10 % ofthe total neutral 17 -ketosteroids. The figures obtained by Salter et al [ 1946], who used similar colorimetrie methods to our own, lie in the region of 15 %. We find higher values than these, so that the pubertal increase in total neutral 17-ketosteroids must therefore be mainly due to substances other than dehydroisoandrosterone.…”

“…In our investigation it is assumed that the difference between the results obtained by the Pincus reaction and those obtained by the Zimmermann reaction will give an approximate measure of the dehydroisoandrosterone. Salter, Cahen & Sappington [1946] have analysed in this way the urines of a heterogeneous collection of normal and abnormal individuals which, however, included only three normal children. We have attempted in our series to determine similarly the proportion of dehydro¬ isoandrosterone .…”

Metabolic Functions of the Endocrine Glands

“…The methods of analysis of urinary 17-ketosteroids have been further described (150,151), and three method3 of determining 17-ketosteroids have been compared (152). Talbot et al (153) have described a colorimetric assay for ll-oxycorticosteroids based on the method of Venning, et al (154).…”

“…Salter et at. (152) fractioned the keto steroids in the urine of normal, endocrine and cancerous subjects and appraised the clinical significance of these methods. Lieber� man & Dobriner (160) identified pregnanediol-32, 17-one-20 from the urine of various patients.…”