Urinary Biomarkers in Interstitial Cystitis/Bladder Pain Syndrome and Its Impact on Therapeutic Outcome

Lin, Hung‐Yu; Lu, Jian‐He; Chuang, Sung-Kiang; Chueh, Kuang-Shun; Juan, Tai-Jui; Liu, Yi-Chang; Juan, Yung‐Shun

doi:10.3390/diagnostics12010075

Cited by 21 publications

(15 citation statements)

References 223 publications

(295 reference statements)

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“…Urinary proteins had long been considered to have a diagnostic role in bladder dysfunction or bladder outlet dysfunctions [ 4 , 5 , 7 , 8 ]. However, owing to variations in urine collection, different renal conditions, and medical comorbidities, urine levels of biomarkers varied widely in the studies of OAB, IC/BPS, and DV-related BOO [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Urine contains proteins and is excreted from the kidney or bladder wall. Previous studies have found that some urinary cytokines, chemokines, and oxidative stress biomarkers are elevated in patients with overactive bladder (OAB), DO, IC/BPS, and DV [ 4 , 5 , 6 , 7 , 8 ]. Although urine-based biomarkers may provide valuable diagnostic and prognostic tools for the clinical assessment of LUTDs and treatment guidelines, the urine proteins currently found in women with different bladder disorders varied widely and are poorly reproducible [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Urinary Oxidative Stress Biomarker Levels Might Be Useful in Identifying Functional Bladder Disorders in Women with Frequency and Urgency Syndrome

Jiang

Jhang

2023

JCM

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Purpose: lower urinary tract dysfunctions (LUTDs) are difficult to diagnose based on symptoms. This study used a cluster of urinary biomarkers, including inflammatory cytokines, neurogenic proteins, and oxidative stress biomarkers, to identify LUTDs in women with frequency and urgency symptoms. Methods: in total, 253 women with video urodynamics (VUDS)- and cystoscopy-confirmed detrusor overactivity (DO), interstitial cystitis/bladder pain syndrome (IC/BPS), dysfunctional voiding (DV), and hypersensitive bladder (HSB), and normal controls were included. Before diagnosis and treatment, urine samples were collected for analysis of biomarkers. The urine levels of biomarkers were compared between groups with bladder dysfunctions and controls and were combined to test the sensitivity in identifying total pathological bladder diseases and specific bladder diseases. Results: After video urodynamic study, VUDS, and urological examinations, bladder dysfunctions were classified into DO (n = 31), IC/BPS (n = 114), DV (n = 45), HSB (n = 29), and control (n = 34) groups. By using a cystomeric bladder capacity of ≤350 mL, 186/219 (84.9%) of the patients with DO, IC/BPS, DV, and HSB can be discriminated from the controls. Among these urine biomarkers, oxidative stress biomarkers 8-isoprostane, 8-hydroxydeoxyguanosine (8-OHdG), or total antioxidant capacity (TAC) are useful for identifying pathological bladder dysfunction (DO, IC/BPS, and DV) and HSB. With elevated IL-1β and lower IL-2, and elevated TNF-α levels, most patients with DV can be identified. Between DO and IC/BPS, a higher NGF level can identify 58.3% of IC/BPS cases, whereas a lower NGF level can identify 75.0% of DO cases. Conclusion: by using a cluster of urine biomarkers, DO, IC/BPS, and DV cases can be identified based on elevated levels of urine oxidative stress biomarkers 8-isoprostane, TAC, or 8-OHdG, and HSB cases with a low TAC. These urine biomarkers are useful for identifying specific LUTDs in women with frequency and urgency symptoms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Urinary Oxidative Stress Biomarker Levels Might Be Useful in Identifying Functional Bladder Disorders in Women with Frequency and Urgency Syndrome

Jiang

Jhang

2023

JCM

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“…The marker 8-OHdG is a common and sensitive biomarker of oxidative DNA damage [ 35 ]. Interestingly, in case of 100 µM NE treatment, the concentration of 8-OHdG was significantly decreased, however some previous papers highlighted that hormonal stress is able to induce oxidative DNA damage (reflected by an elevated 8-OHdG level) in human oral keratinocyte cells, and also in hepatocytes of rats [ 36 , 37 ]; further, an increased 8-OHdG level was detected in the urine of patients with interstitial cystitis [ 38 , 39 ]. Based on these previous data, the above-mentioned paradoxical effect may be explained rather by the over-compensating processes of uroepithelial cells, than the protective role of NE against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Feline Uroepithelial Cell Culture as a Novel Model of Idiopathic Cystitis: Investigations on the Effects of Norepinephrine on Inflammatory Response, Oxidative Stress, and Barrier Function

Hatala

Lajos

Mackei

et al. 2023

Veterinary Sciences

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Feline idiopathic cystitis (FIC) is one of the most common urinary tract disorders in domestic cats. As stress is suggested to play a key role in the pathogenesis of FIC, the effects of norepinephrine (NE) as a stress mediator were investigated on a novel feline primary uroepithelial cell culture, serving as an in vitro model of the disease. The uroepithelial cells gained from the mucosa of the bladder of a euthanized cat were cultured for 6 days and were acutely exposed to NE (10, 100, and 1000 µM) for 1 h. NE increased the metabolic activity of the cultured cells and elevated the extracellular concentrations of the pro-inflammatory mediators interleukin-6 (IL-6) and stromal cell derived factor 1 (SDF-1), confirming that NE can trigger an inflammatory response in the uroepithelium. Cellular protein carbonyl levels were increased by NE exposure, while malondialdehyde and glucose regulated protein 78 concentrations remained unchanged, indicating that NE may provoke the oxidative damage of proteins without inducing lipid peroxidation or endoplasmic reticulum stress. Further, it can be strongly suggested that an acute NE challenge might diminish the barrier function of uroepithelial cells, as reflected by the decreased glycosaminoglycan concentration, claudin-4 protein expression, and reduced TER values of the NE-treated cell cultures. Based on these results, short-term NE exposure mimicking acute stress can provoke an inflammatory response and decrease the barrier integrity of cultured feline uroepithelial cells. Hence, it is highly expected that stress-associated NE release may play an important mediatory role in the pathogenesis of FIC.

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“…Although researchers have made a great effort to investigate the pathogenesis of UTI, most studies have been carried out using various animal models, and the evidence from human bladders remains limited 13 . Recent research has suggested using intravesical PRP injection might have a therapeutic effect in treating lower urinary tract disorders due to regenerative deficiency 14 .…”

Section: Discussionmentioning

confidence: 99%

Conditions Diagnosed on Bladder Biopsy in Adult Female Patients with Lower Urinary Tract Symptoms (LUTS) Refractory to Medical Treatment

Rehman¹,

Ahmad²,

Sahi³

et al. 2022

PJMHS

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Introduction: The incidence of lower urinary tract symptoms (LUTS) as the sole presenting symptom for bladder cancer has traditionally been reported to be low. Refractory LUTS are a well-recognized initial presenting symptom in a small percentage of patients with newly diagnosed bladder cancer. Traditionally, patients who present with refractory LUTS are thought to have carcinoma in-situ (CIS) at diagnosis. Objectives: The objective of this study was to evaluate the evaluate the Conditions diagnosed on bladder biopsy in adult female patients with lower urinary tract symptoms (LUTS) refractory to medical treatment. Materials and Methods: We queried our database of bladder cancer patients in the hospital to identify patients who presented solely with LUTS and were subsequently diagnosed with bladder cancer. Demographic, clinical, and pathologic variables were examined. Results: 4.1% (14/340) of bladder cancer patients in our series presented solely with LUTS. Mean age and Charlson Co-morbidity Index of these patients was 66.4 years. Of the 14 patients in our data presenting with LUTS, 9 (64.3%), 4 (28.6%), and 1 (7.1%) patients presented with clinical stage Ta, carcinoma in Situ (CIS), and T2 disease. At a follow up of 6 months, recurrence occurred in 7 (50.0%) patients with progression occurring in 1 (7.1%) patient. 11 (78.6%) patients were alive and currently disease free, and 3 (21.4%) patients had died, with only one (7.1%) death attributable to bladder cancer. Practical implication: This study suggests that urologists should have a low threshold for evaluating patients with unexplained LUTS for underlying bladder cancer. Conclusions: It is concluded that incidence of LUTS as the sole presenting symptom in patients with newly diagnosed bladder cancer. Keywords: UTI, Bladder, Patients, Hyperplasia, LUTS, Cancer

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Urinary Biomarkers in Interstitial Cystitis/Bladder Pain Syndrome and Its Impact on Therapeutic Outcome

Cited by 21 publications

References 223 publications

Urinary Oxidative Stress Biomarker Levels Might Be Useful in Identifying Functional Bladder Disorders in Women with Frequency and Urgency Syndrome

Urinary Oxidative Stress Biomarker Levels Might Be Useful in Identifying Functional Bladder Disorders in Women with Frequency and Urgency Syndrome

Feline Uroepithelial Cell Culture as a Novel Model of Idiopathic Cystitis: Investigations on the Effects of Norepinephrine on Inflammatory Response, Oxidative Stress, and Barrier Function

Conditions Diagnosed on Bladder Biopsy in Adult Female Patients with Lower Urinary Tract Symptoms (LUTS) Refractory to Medical Treatment

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