Urinary bladder smooth muscle regeneration utilizing bone marrow derived mesenchymal stem cell seeded elastomeric poly(1,8-octanediol-co-citrate) based thin films

Sharma, Arun K.; Hota, Partha; Matoka, Derek J.; Fuller, Natalie J.; Jandali, Danny; Thaker, Hatim; Ameer, Guillermo A.; Cheng, Earl Y.

doi:10.1016/j.biomaterials.2010.04.054

Cited by 129 publications

(126 citation statements)

References 38 publications

(52 reference statements)

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“…Data from this study indicate that bladder reconstitution occurs more robustly using epitope-defined MSCs compared with normal human bSMCs seeded on an elastomeric scaffold compared with unseeded control animals. MSC-seeded scaffolds maintained high levels of smooth muscle markers and displayed smooth muscle bundle formation by trichrome staining with statistically superior muscle to collagen ratios at 10 weeks postimplantation compared with bSMCs-seeded scaffold constructs [9]. This data implies that MSCs can serve as an alternative cell source for potentially diseased bSMCs.…”

Section: Introductionmentioning

confidence: 76%

“…Further data presented in this study demonstrate both cell types can also respond to agonist stimulation with statistically indistinguishable contractile responses in vitro. A second study describes the use of MSCs in a nude rat bladder augmentation model [9]. Data from this study indicate that bladder reconstitution occurs more robustly using epitope-defined MSCs compared with normal human bSMCs seeded on an elastomeric scaffold compared with unseeded control animals.…”

Section: Introductionmentioning

confidence: 93%

“…Experimental data has demonstrated the utility of bone marrow (BM) cells in a bladder regenerative setting with data suggesting the phenotypic and physiological similarities between mesenchymal stem cells (MSCs) and bSMCs [7][8][9]. The characterization of a battery of contractile proteins shows that MSCs have a contractile protein profile similar to bSMCs [8].…”

Section: Introductionmentioning

confidence: 99%

“…Studies describing this phenomenon in nude rats demonstrate the rapid invasion of native urothelium onto the luminal side of cystectomized bladders that have been seeded with cells. This rapid invasion of urothelium may prevent the luminally seeded cell populations from proliferating and contributing to the grafts [9,12]. Alternatively, the cells on the luminally seeded side of the graft may undergo cell death as a consequence of being in contact with urine.…”

Section: Introductionmentioning

confidence: 99%

“…The limited graft size in rodent models provides an inadequate model of regeneration due to the rate at which the regenerative process occurs. Small augmented areas tend to regenerate within a matter of weeks, whereas larger grafts, as seen in canine models, can take up to several months to regenerate completely [9,14,15]. More importantly, there exist anatomical differences between the lower nonhuman primates (NHPs) species and humans that include the musculature component of the lower urinary tract further questioning the translational validity of lower NHP models [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

et al. 2011

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Animal models that have been used to examine the regenerative capacity of cell-seeded scaffolds in a urinary bladder augmentation model have ultimately translated poorly in the clinical setting. This may be due to a number of factors including cell types used for regeneration and anatomical/ physiological differences between lower primate species and their human counterparts. We postulated that mesenchymal stem cells (MSCs) could provide a cell source for partial bladder regeneration in a newly described nonhuman primate bladder (baboon) augmentation model. Cell-sorted2 baboon MSCs transduced with green fluorescent protein (GFP) were seeded onto small intestinal submucosa (SIS) scaffolds. Baboons underwent an approximate 40%-50% cystectomy followed by augmentation cystoplasty with the aforementioned scaffolds or controls and finally enveloped with omentum. Bladders from sham, unseeded SIS, and MSC/SIS scaffolds were subjected to trichrome, H&E, and immunofluorescent staining 10 weeks postaugmentation. Immunofluorescence staining for muscle markers combined with an anti-GFP antibody revealed that >90% of the cells were GFP Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Introductionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

et al. 2011

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Directional Freeze‐Casting: A Bioinspired Method to Assemble Multifunctional Aligned Porous Structures for Advanced Applications

2020

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Mohammad-Ali Shahbazi received his Ph.D. in 2015 from University of Helsinki, Finland, where he worked on porous materials for drug delivery to cancer tissues. He is currently a postdoc scientist at Faculty of Pharmacy, University of Helsinki, working on therapeutic microdevices for autoimmune diseases. He is an expert in oral peptide delivery and fabrication of cell-mimicking carriers. His current research interest includes nano-based regenerative hydrogels for wound healing, bone repair, and long-term drug delivery.

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A Review of Bioresorbable Implantable Medical Devices: Materials, Fabrication, and Implementation

Singh

Bathaei

Istif

et al. 2020

Adv Healthcare Materials

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Implantable medical devices (IMDs) are designed to sense specific parameters or stimulate organs and have been actively used for treatment and diagnosis of various diseases. IMDs are used for long‐term disease screening or treatments and cannot be considered for short‐term applications since patients need to go through a surgery for retrieval of the IMD. Advances in bioresorbable materials has led to the development of transient IMDs that can be resorbed by bodily fluids and disappear after a certain period. These devices are designed to be implanted in the adjacent of the targeted tissue for predetermined times with the aim of measurement of pressure, strain, or temperature, while the bioelectronic devices stimulate certain tissues. They enable opportunities for monitoring and treatment of acute diseases. To realize such transient and miniaturized devices, researchers utilize a variety of materials, novel fabrication methods, and device design strategies. This review discusses potential bioresorbable materials for each component in an IMD followed by programmable degradation and safety standards. Then, common fabrication methods for bioresorbable materials are introduced, along with challenges. The final section provides representative examples of bioresorbable IMDs for various applications with an emphasis on materials, device functionality, and fabrication methods.

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Urinary bladder smooth muscle regeneration utilizing bone marrow derived mesenchymal stem cell seeded elastomeric poly(1,8-octanediol-co-citrate) based thin films

Cited by 129 publications

References 38 publications

A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

Directional Freeze‐Casting: A Bioinspired Method to Assemble Multifunctional Aligned Porous Structures for Advanced Applications

A Review of Bioresorbable Implantable Medical Devices: Materials, Fabrication, and Implementation

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