Urinary catheter indwelling clinical pathogen biofilm formation, exopolysaccharide characterization and their growth influencing parameters

Murugan, Kasi; Krishnasamy, Selvanayaki; Al-Sohaibani, Saleh

doi:10.1016/j.sjbs.2015.04.016

Cited by 40 publications

(32 citation statements)

References 42 publications

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“…Were isolated. In contrast to our study, S.aureus (24%), P.aeruginosa (18%), E.faecalis (14%) and others (44%) were identified as etiological agents by Murugan [16]. In present study, the most resistant antibiotic were Ofloxacin followed by Co-Trimoxazole and isolates were mostly susceptible to Amikacin followed by Ceftizoxime antibiotics.…”

Section: Fig5 Frequency Wise Distribution Of Isolates F Antimicrobicontrasting

confidence: 92%

Current Status of Urinary/Cardiac Catheter Indwelling Pathogens and their Biofilm Formation Capabilities

Gamit¹

2019

IJRASET

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A great threat to indwelling devices like urinary and cardiac catheters is by an accumulation of microorganisms embedded in an exopolysaccharide matrix. This may result into slow and persistent infections and thereby interfering with antibiotic therapy too. This study was aimed to find out the bacterial and fungal etiological agents that are the major cause of biofilm formation on tips of urinary and cardiac catheters along with their higher resistance towards antimicrobials. Total 36 urinary catheter tips and 4 cardiac catheter tips were collected from various clinics of Surat region, since December 2018 to March 2019 from the admitted patients. Out of these samples, many of them were polymicrobials and produced total of 63 isolates. Among these isolates, 93.66% were bacterial etiological agents and 6.34% were fungal etiological agents. Gram negative isolates predominated (55.56%) over gram positives. Antibiotics susceptibility patterns of isolates were determine by Kirby Bauer disc diffusion method. Prevalence of ESBLs producing gram negative isolates and determination of Oxacillin resistant gram Positive cocci (S.aureus) were carried out. Biofilm forming capabilities were observed in majority of the isolates when performed by Tube, Congo Red Agar (CRA), and Microtitre Plate (MTP) Methods. Partial molecular sequencing (18S r RNA) of screened isolate with high biofilm formation capabilities and its phylogenetic analysis is determined.

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Section: Fig5 Frequency Wise Distribution Of Isolates F Antimicrobicontrasting

confidence: 92%

Current Status of Urinary/Cardiac Catheter Indwelling Pathogens and their Biofilm Formation Capabilities

Gamit¹

2019

IJRASET

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“…The inhibitory effect of E. gunnii EO against S. aureus confirmed the capability of the EOs recovered by species belonging to the Eucalyptus genus to block the biofilm formation by this methicillin resistant strain (Junka et al, 2019); its activity was more powerful than E. globulus EO (Merghni et al, 2018). The effectiveness shown by both EOs could be taken into consideration, once again, in patient management, mainly to avoid S. aureus infections that concern the central venous, dialysis (Gahlot et al, 2014), and urinary catheters (Murugan et al, 2016), as well as arteriovenous shunts (MacRae et al, 2016) and sutures (Henry-Stanley et al, 2010). Interestingly, L. petersonii EO at 0.5 µg/mL induced a strong inhibition of S. aureus biofilm formation (79.88%), while maintaining a high metabolic activity (90.89%) compared to the control.…”

Section: Biofilm and Metabolic Activity Of Biofilm Cellsmentioning

confidence: 55%

Chemical Composition and Biological Activities of the Essential Oils of Leptospermum petersonii and Eucalyptus gunnii

et al. 2020

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The aim of this study was to characterize the chemical composition and to evaluate the antimicrobial and phytotoxic properties of the essential oils (EOs) obtained from leaves of Leptospermum petersonii chemotype "Variety B" and Eucalyptus gunnii, native to Australia. Geranyl acetate, γ-terpinene, geraniol, terpinolene, α-pinene, p-cimene, and linalool were the main components in L. petersonii EO, confirming also the existence of several chemotypes in such taxa; on the other hand, 1,8-cineole, trans-sabinene hydrate acetate, globulol, longicyclene, terpinolene, and camphene were present in major amounts in the E. gunnii EO. Chemical analysis of L. petersonii revealed that it belongs to the variety "B." E. gunnii EO showed good antibacterial activity, with an MIC of 0.5 and 2 µg/mL against Staphylococcus aureus, and Pectobacterium carotovorum, respectively. The activity of E. gunnii EO was stronger than L. petersonii EO, whose maximum MIC reached 5 µg/mL. E. gunnii and L. petersonii EOs were particularly effective in inhibiting the biofilm formation by S. aureus, already at a concentration of 0.01 µg/mL. The other strains were resistant to both EOs up to a dose of 0.05 µg/mL. The maximum inhibition on biofilm formed by P. carotovorum was recorded for E. gunnii EO, reaching a value of 93.12% at 1.0 µg/mL. This is the first manuscript which studies the biofilm inhibition by EOs and evaluates their effects on biofilm metabolism. Both EOs were more effective against P. carotovorum. In addition, even though L. petersonii EO 0.1 µg/mL was unable to inhibit biofilm formation by Escherichia coli, it decreased the metabolic activity of the biofilm to 78.55% compared to control; furthermore, despite it inducing a relatively low inhibition (66.67%) on biofilm formation, it markedly affected metabolic activity, which decreased to 16.09% with respect to the control. On the contrary, L. petersonii EO 0.5 µg/mL induced a 79.88% inhibition of S. aureus

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“…The high incidence of CAUTI at KRRH may be attributed to poor hygiene practices in relation to catheter handling, placement and maintenance. In addition previous studies have documented poor surveillance of hospital acquired infections, neglect of patient safety and health care quality as contributors to the high incidences of hospital acquired infections like CAUTI [19]. In a related study done in Zambia, a higher incidence of CAUTI (35%) was reported [6], however, the duration of catherisation was longer (28days) implying that the longer a catheter stays in-situ, the more likely it is to lead to an infection within the urinary tract as reported in other studies [10].…”

Section: Discussionmentioning

confidence: 99%

Incidence and Etiology of Catheter Associated Urinary Tract Infection among Admitted Patients at Kabale Regional Referral Hospital, South Western Uganda

Musinguzi¹,

Kabajulizi²,

Mpeirwe³

et al. 2019

AID

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Introduction: Catheter Associated Urinary Tract Infection is the most common hospital acquired infection worldwide. Urinary Tract Infections among catheterised patients are on rise regardless of antibiotic use and this is due to erratic use of antibiotics, treatment failure, antimicrobial resistance and emergency of Extended Spectrum Beta Lactamase producing bacteria leading to patient distress, increased healthcare costs, long hospital stay and poor patient response to antibiotics. In Uganda, no previous studies have sought to study the burden of CAUTI among catheterized patients, the bacterial pathogens involved and their antimicrobial susceptibility patterns yet there is upsurge in antimicrobial resistance of uropathogens. The effective management of patients suffering from Catheter Associated Urinary Tract infection (CAUTI) relays on the identification of uropathogens that cause CAUTI and the selection of an effective antibiotic agent to the uropathen in question. Objectives: The objectives of this study were to determine incidence, etiology and antibiotic susceptibility pattern among the uropathogens causing Catheter Associated Urinary Tract Infections among patients with indwelling catheters at Kabale Regional Referral Hospital. Methods: Using a descriptive prospective observational hospital-based study, the study was conducted on 150 catheterized patients recruited from Emergency, Obstetrics and gynecology, Medical, Maternity and Surgical wards at Kabale Regional Referral Hospital between April and May 2019. The urine samples from study participants were processed in Kabale RRH microbiology laboratory as per standard operating procedures. After isolation and identification, all the isolates were subjected How to cite this paper: Musinguzi, B.,

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Urinary catheter indwelling clinical pathogen biofilm formation, exopolysaccharide characterization and their growth influencing parameters

Cited by 40 publications

References 42 publications

Current Status of Urinary/Cardiac Catheter Indwelling Pathogens and their Biofilm Formation Capabilities

Current Status of Urinary/Cardiac Catheter Indwelling Pathogens and their Biofilm Formation Capabilities

Chemical Composition and Biological Activities of the Essential Oils of Leptospermum petersonii and Eucalyptus gunnii

Incidence and Etiology of Catheter Associated Urinary Tract Infection among Admitted Patients at Kabale Regional Referral Hospital, South Western Uganda

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