Urinary Elimination of Estrogens Injected in Dogs

Canine Ovarian Tumours Following Stilboestrol Administration

“…It is now clear that, as in other animals, the liver is responsible for the inaetivation of naturally occurring oestrogens in the dog (Lipsehutz, 1950;Cantarow, 1942;Cantarow, Paschkis, Rakoff and Hansen, 1943a;Cantarow, Paschkis, Rakoff, Hansen and Walkling, 1943b;Dingemanse and Tyslowitz, 1941;Longwell and McKee, 1942). In support of this, Israel et al (Cantarow et al, 1943a) in 1937 found that oestrone was not inactivated when perfu.sed tlirough a Jieartlung preparation from a dog, but was inactivated within fifteen minutes when perfused through a heart-Iung-liver preparation.…”

“…However, a striking (Hfference in metabolism is apparent in dogs when synthetic oestrogens, such as stilboestrol, are administered. The recovery of active oestrogen from the bile of dogs was considerably lower with dietliylstilboestrol administration, whilst increased activity was still found in the blood even after two days (Cantarow et al, 1943b;Dingemanse and Tyslowitz, 1941). In the urine 8-25 p.c.…”

THE DIETHYLSTILBESTROL CONTENT OF THE MEAT FROM CHICKENS TREATED WITH THIS ESTROGENIC SUBSTANCEa

“…However, the large amount of interfering substances extracted from tissue by acetone gave trouble. The color reaction of Dingemanse (3) and Dingemanse and Tyslowitz ( 4 ) reports and that of Dodgson et a2. ( 5 ) it appeared advisable to attempt to determine the glucurouide of diethylstilbestrol as well as the free compound.…”

The colorimetric estimation of stilboestrol, hexoestrol and their glucuronides in urine

“…The very low recoveries of conjugated oestrogen reported hitherto from the urines of experimental aniimals (Stroud, 1939;Dingemanse & Tyslowitz, 1941;Bass & Salter, 1943) have undoubtedly been due to the failure to appreciate the degree of solubility of the glucuronides in organic solvents, and, more especially, the losses entailed when hydrolysis is performed on urine itself, rather than on suitably prepared extracts.…”

“…Sahasrabudhe & Wilder Smith (1947) have largely overcome this problem in the case of dienoestrol by taking advantage of its diene structure and its reactivity with maleic anhydride. For the two more stable oestrogens, stilboestrol and hexoestrol, it appears that no satisfactory method of estimation in the presence of urine has yet been proposed, though attempts have been made to apply the Dingemanse (1940) method to this problem (Dingemanse & Tyslowitz, 1941;Bass & Salter, 1943). The present communication describes methods of preliminaryextraction which enable estimations of these two clinically important oestrogens and of their glucuronides to be made in urine by the nitration method proposed by Malpress (1945).…”