Urinary excretion of 8‐hydroxy‐2‐deoxyguanosine and 1‐hydroxypyrene in coke‐oven workers

Abstract

Coke-oven workers (COWs) are occupationally exposed to high concentrations of polycyclic aromatic hydrocarbons (PAH). Urinary 8-hydroxy-2-deoxyguanosine (8-OH-dG) and 1-hydroxypyrene (1-OHP) are biological markers of oxidative DNA damage and PAH metabolism, respectively. We investigated the relationship between urinary 8-OH-dG and 1-OHP in 217 Taiwanese COWs, 55 topside-oven, and 162 sideoven workers. For topside-oven workers, mean 8-OH-dG and 1-OHP concentrations (ng/ml +/- SD) were 13.8 +/- 12.0 and 93.5 +/- 104.4, respectively. These levels were significantly higher than those for sideoven workers: 10.2 +/- 7.9 ng/ml (P = 0.04) and 19.8 +/- 28.6 ng/ml (P < 0.001), respectively. Individual urinary 8-OH-dG concentrations were directly correlated with urinary 1-OHP concentrations: the higher the 1-OHP level, the higher the 8-OH-dG level (Spearman correlation coefficients: r = 0.43, P < 0.0001, n = 217). Multiple regression analysis indicated that a 10-fold increase in 1-OHP was associated with a 1.91-fold increase in 8-OH-dG. Compared to no vitamin intake, intake of at least one multiple vitamin pill per week reduced 8-OH-dG excretion (P = 0.02). Our findings suggest that urinary 1-OHP and 8-OH-dG reflect occupational PAH exposure and oxidative DNA damage in COWs. In addition, multiple vitamins may reduce oxidative stress caused by PAH exposure.

Effect of vitamin levels on biomarkers of exposure and oxidative damage—The EXPAH study

“…Similar findings were reported by a study conducted in five European countries (31), and by a study involving men on high fruit and vegetable diets (32). A study involving coke-oven workers who took vitamin supplements showed that the excretion of 8-oxodG adducts was decreased compared to those workers that took no vitamins (33). However, the study did not include direct measurements of plasma vitamin levels were not measured.…”

The relationship between biomarkers of oxidative DNA damage, polycyclic aromatic hydrocarbon DNA adducts, antioxidant status and genetic susceptibility following exposure to environmental air pollution in humans

“…These findings conflict with the generally accepted consensus of opinion that an intake of fruits and vegetables by healthy individuals leads to a reduction of oxidative DNA damage [35]. A study involving coke-oven workers who took at least one multi vitamin pill showed that the urinary excretion of 8-oxodG adducts was decreased compared to those workers that took no vitamins [36]. In a separate study the level of M 1 dG adducts was found to be decreased in human colorectal mucosa DNA from men that had a diet with high levels of fruit and vegetables [37].…”

Repeated Measurements of Urinary Methylated/Oxidative DNA Lesions, Acute Toxicity, and Mutagenicity in Coke Oven Workers

“…Notably, most previous studies measured urinary and/or blood biomarkers at the end of a work shift as a single measurement (5,7,26,32,33). These readings may be affected by the known circadian variation in certain biomarker level (e.g., 8-oxodG; ref.…”

“…Urinary1-OHP was determined by a high performance liquid chromatography (HLPC) with the fluorescence detection method (26). Briefly, 20 mL of 0.1 mol/L acetate buffer (pH 5.0) and 20 AL glucoronidase-arylsulfatase (134,600 unit/mL; Sigma) were added to 10 mL urine and incubated for 4 h at 37jC in an electronically controlled rotary shaking bath.…”