Urinary Excretion of Lysozyme and N-Acetyl-β-D-Glucosaminidase in the Diagnosis of Renal Allograft Rejection

Abstract

Garcia et al., 1975). The purpose of this study was to assess whether the measurements of both enzymes gave earlier prediction of graft rejection than either alone. URINE SAMPLESFor enzyme assay, freshly voided samples were collected and assayed, usually within 48 hours. No preservatives were used and, when the assays were not performed immediately, the specimens were stored at 4°C or -20°C. Both lysozyme and NAG are very stable enzymes in the urine (Burrows, 1967;Harrison et al., 1968; Shehadeh et al., 1970;Zucker and Webb, 1970; Sandman et al., 1973;Wellwood et al., 1973). Annals of Clinical Biochemistry, 1978, 15, 253-260 have been made in 35 patients after renal transplantation, and during 52 suspected rejection episodes. A close correlation has been found between these two parameters although they did not consistently change in parallel. The use of both assays may give a better indication of impending rejection than either assay alone.

Transcriptome analysis of renal ischemia/reperfusion (I/R) injury in BAFF and BAFF-R deficient mice

“…Alterations in urine and serum levels of lysozyme were detected in acute renal failure [74]. Up to 1988 several publications discussed the occurrence and role of lysozyme in context of renal (transplant) damage and failure, also in view of suitability as diagnostic marker [75][76][77][78][79][80][81][82][83]. First report, that lysozyme, intravenously infused into male rats, induced functional as well as structural alterations and finally caused acute renal failure was presented by Cojocel et al [84].…”