Urinary extracellular vesicles signature for diagnosis of kidney disease

Takizawa, Keiichi; Ueda, Koji; Sekiguchi, Mutsuo; Nakano, Eiji; Nishimura, Tōru; Kajiho, Yuko; Kanda, Shoichiro; Miura, Kenichiro; Hattori, Motoshi; Hashimoto, Junya; Hamasaki, Yuko; Hisano, Masataka; Omori, Tae; Okamoto, Takayuki; Kitayama, Hirotsugu; Fujita, Naoya; Kuramochi, Hiromi; Ichikı, Takanori; Oka, Akira; Harita, Yutaka

doi:10.1016/j.isci.2022.105416

Cited by 12 publications

(24 citation statements)

References 53 publications

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“…Using nanoparticle tracking analysis and quantitative proteomics, differentially expressed proteins in uEVs were identified in bilateral kidney hypoplasia, which is characterized by a congenitally reduced number of nephrons. This uEV expression signature reflected decreased kidney function in CKD patients due to congenital kidney and urinary tract disease [16]. Distal tubule and collecting duct-specific MUC1 was one of the molecules found to be reduced in uEVs in kidney hypodysplasia.…”

Section: Kidney Hypoplasiamentioning

confidence: 86%

“…In addition, in kidney hypodysplasia, uEVs had higher levels of proximal tubule-specific maltase-glucoamylase (MGAM). Although the validation cohort did not show a statistically significant increase in MGAM in CKD, it is thought to capture a different aspect of uEVs from decreased MUC1 expression and thus improves diagnostic ability when combined with MUC1 [16]. MGAM, an α-glucosidase, acts as an enzyme in the final step of starch digestion, converting linear regions of starch to glucose [153].…”

Section: Kidney Hypoplasiamentioning

confidence: 96%

“…MGAM, an α-glucosidase, acts as an enzyme in the final step of starch digestion, converting linear regions of starch to glucose [153]. Increased expression of MGAM, like other proximal tubule markers, may be associated with compensatory responses in proximal tubular cells [16].…”

Section: Kidney Hypoplasiamentioning

confidence: 99%

“…Urinary EVs (uEVs) are thought to contain only about 3% of urine proteins. As a result, potential interesting biomarkers contained in EVs may simply go undetected due to dilution in whole urine [16,17]. Because of the strong correlation between kidney and uEV protein abundances, uEVs are an appealing noninvasive source of biomarkers for research into kidney physiology or disease [18]: for example, uEVs provide a noninvasive method for studying tubular transport in health and disease [19].…”

Section: Introductionmentioning

confidence: 99%

“…Because of the strong correlation between kidney and uEV protein abundances, uEVs are an appealing noninvasive source of biomarkers for research into kidney physiology or disease [18]: for example, uEVs provide a noninvasive method for studying tubular transport in health and disease [19]. Thus, determining biomarkers specifically related to urinary EVs may be of interest in detecting changes in the kidney system [16,17].…”

Section: Introductionmentioning

confidence: 99%

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Urinary Extracellular Vesicles in Chronic Kidney Disease: From Bench to Bedside?

et al. 2023

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Extracellular vesicles are a diverse group of particles that include exosomes, microvesicles, and apoptotic bodies and are defined by size, composition, site of origin, and density. They incorporate various bioactive molecules from their cell of origin during formation, such as soluble proteins, membrane receptors, nucleic acids (mRNAs and miRNAs), and lipids, which can then be transferred to target cells. Extracellular vesicles/exosomes have been extensively studied as a critical factor in pathophysiological processes of human diseases. Urinary extracellular vesicles could be a promising liquid biopsy for determining the pattern and/or severity of kidney histologic injury. The signature of urinary extracellular vesicles may pave the way for noninvasive methods to supplement existing testing methods for diagnosing kidney diseases. We discuss the potential role of urinary extracellular vesicles in various chronic kidney diseases in this review, highlighting open questions and discussing the potential for future research.

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Section: Kidney Hypoplasiamentioning

confidence: 86%

Section: Kidney Hypoplasiamentioning

confidence: 96%

Section: Kidney Hypoplasiamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Urinary Extracellular Vesicles in Chronic Kidney Disease: From Bench to Bedside?

et al. 2023

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Proteomics of urinary small extracellular vesicles in early diagnosis of kidney diseases in children‐expectations and limitations

Korecka,

Gawin,

Pastuszka

et al. 2024

Proteomics

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The primary function of the kidneys is to maintain systemic homeostasis (disruption of renal structure and function results in multilevel impairment of body function). Kidney diseases are characterized by a chronic, progressive course and may result in the development of chronic kidney disease (CKD). Evaluation of the composition of the proteome of urinary small extracellular vesicles (sEVs) as a so‐called liquid biopsy is a promising new research direction. Knowing the composition of sEV could allow localization of cellular changes in specific sections of the nephron or the interstitial tissue before fixed changes, detectable only at an advanced stage of the disease, occur. Research is currently underway on the role of sEVs in the diagnosis and monitoring of many disease entities. Reports in the literature on the subject include: diabetic nephropathy, focal glomerulosclerosis in the course of glomerulopathies, renal fibrosis of various etiologies. Studies on pediatric patients are still few, involving piloting if small groups of patients without validation studies. Here, we review the literature addressing the use of sEV for diagnosis of the most common urinary disorders in children. We evaluate the clinical utility and define limitations of markers present in sEV as potential liquid biopsy.

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Extracellular vesicles in kidney development and pediatric kidney diseases

Ergunay,

Collino,

Bianchi

et al. 2023

Pediatr Nephrol

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Extracellular vesicles (EVs) are membranous cargo particles that mediate intercellular communication. They are heterogeneous in size and mechanism of release, and found in all biological fluids. Since EV content is in relation to the originating cell type and to its physiopathological conditions, EVs are under study to understand organ physiology and pathology. In addition, EV surface cargo, or corona, can be influenced by the microenvironment, leading to the concept that EV-associated molecules can represent useful biomarkers for diseases. Recent studies also focus on the use of natural, engineered, or synthetic EVs for therapeutic purposes. This review highlights the role of EVs in kidney development, pediatric kidney diseases, including inherited disorders, and kidney transplantation. Although few studies exist, they have promising results and may guide researchers in this field. Main limitations, including the influence of age on EV analyses, are also discussed.

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Urinary extracellular vesicles signature for diagnosis of kidney disease

Cited by 12 publications

References 53 publications

Urinary Extracellular Vesicles in Chronic Kidney Disease: From Bench to Bedside?

Urinary Extracellular Vesicles in Chronic Kidney Disease: From Bench to Bedside?

Proteomics of urinary small extracellular vesicles in early diagnosis of kidney diseases in children‐expectations and limitations

Extracellular vesicles in kidney development and pediatric kidney diseases

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