Urinary malondialdehyde and 8-hydroxydeoxyguanosine as potential markers of oxidative stress in industrial art glass workers

Abstract

Previous epidemiological studies have indicated that industrial art glass workers have increased mortality risks for certain types of cancer and for cardio- and cerebrovascular disease. To test the hypothesis that increased oxidative stress might contribute to these increased mortality risks, the urinary levels of the lipid peroxidation product, malondialdehyde (MDA), and the oxidative DNA adduct, 8-hydroxydeoxyguanosine (8OHdG) were determined in 343 workers (230 men and 113 women) from the art glass industry in the southeast of Sweden. Of the study subjects, 199 (181 men and 18 women) were engaged in the process of glass production and were regarded as exposed, whereas the remainders performed clerical, warehouse and other service work and were regarded as unexposed. One hundred and sixteen were smokers (75 men and 41 women) and 215 were non-smokers (142 men and 73 women). The findings indicate that (a) exposure to industrial art glass work per se does not cause any major oxidative stress as measured by urinary levels of MDA and 8OHdG, (b) the effects from smoking per se are limited to increased lipid peroxidation among men, and (c) joint exposure to industrial art glass work and smoking may cause increased lipid peroxidation among men and increased DNA hydroxylation among both men and women. While these findings provide no evidence for increased oxidative stress due to industrial art glass work per se, the increased 8OHdG excretion, in workers who smoke may be associated with a higher risk of developing free radical-dependent degenerative diseases including cancer.

The Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature

“…It has also been widely used as a health effect marker for workers who have been exposed to chemicals [4][5][6][7][8][9] . Urinary 8-OHdG is stable when stored at 4°C for 19 days 10) , and also after 6 years of storage at Field Study -20°C 11) , but to our knowledge there is no report about its stability at room temperature, that is 25°C.…”

Oxidative Stress Associated with Indoor Air Pollution and Sick Building Syndrome-Related Symptoms among Office Workers in Taiwan

“…As a result of exposure to ROS, 8-OHdG can be excised with hydroxylation by constitutive enzymatic repair systems and excreted in the urine (Shigenaga et al, 1989;Floyd et al, 1990;Fraga et al, 1990). Urban air pollution, tobacco smoke, environmental tobacco smoke (ETS), VOCs, and ionization radiation have been associated with the rise of urinary 8-OHdG levels (Samet et al, 1987;Anderson et al, 1991;Wilson et al, 1993;Tagesson et al, 1995Tagesson et al, , 1996Calderon-Garciduenas et al, 1999;Carstensen et al, 1999).…”

HETA 98-0139-2769, The Society of Glass Beadmakers, Corning, New York.

“…Epidemiological studies have indicated that industrial art glass workers have increased mortality risks for certain types of cancer (stomach, colon, lung, skin, and brain) and for c ar di o va s c u l a r a n d c e r e b r o va s c u l a r disease. 11,12,13,14,15,16,17 The International Agency for Research on Cancer (IARC) has determined that the manufacture of art glass entails exposures that are probably carcinogenic to humans. 18 Glass blowing is one major occupation in the art glass industry which shows an increased cancer risk, possibly because the blow pipe introduces the potential for oral exposure.…”