Urinary metabonomics elucidate the therapeutic mechanism of Orthosiphon stamineus in mouse crystal-induced kidney injury

Gao, Songyan; Chen, Wei; Peng, Zhongjiang; Li, Na; Su, Li; Lv, Diya; Li, Ling; Lin, Qishan; Dong, Xin; Guo, Zhiyong; Lou, Ziyang

doi:10.1016/j.jep.2015.03.025

Cited by 27 publications

(20 citation statements)

References 37 publications

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“…Carnitine is an essential cofactor required to transport fatty acids into the inner mitochondrial matrix to provide energy through β-oxidation. The decrease of propionylcarnitine, a product of the enzymatic esterification of carnitine, was observed in mousecrystal-induced kidney injury 54 . Unlike that, in our current study, the levels of propionylcarnitine and Butyrylcarnitinewere significantly increased after CDDP administration, and HI could effectively restore this up-regulation.…”

Section: Discussionmentioning

confidence: 98%

Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats

Song

Wang

et al. 2017

Sci Rep

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The side effects of cisplatin (CDDP), notably nephrotoxicity, greatly limited its use in clinical chemotherapy. HuangQi Injections (HI), a commonly used preparation of the well-known Chinese herbal medicine Astragali radix, appeared to be promising treatment for nephrotoxicity without compromising the anti-tumor activity of CDDP. In this study, the urinary metabolomics approach using liquid chromatography time of flight mass spectrometry (LC-TOF/MS) was developed to assess the toxicity-attenuation effects and corresponding mechanisms of HI on CDDP-exposed rats. As a result, successive administration of HI significantly recovered the decline of body weight and downregulated the abnormal increase of serum creatinine and urea. HI partly restored the CDDP-induced alteration of metabolic profiling back into normal condition. Totally 43 toxicity-attenuation potential biomarkers were screened and tentatively identified, which were involved in important metabolic pathways such as amino acid metabolism, TCA cycle, fatty acid metabolism, vitamin B6 metabolism and purine metabolism. The results clearly revealed that HI could alleviate CDDP-induced nephrotoxicity and improve the disturbed metabolic balance induced by repeated CDDP exposure. The present study provided reliable evidence for the protective effect of HI on CDDP-induced toxicity with the multi-target pharmacological characteristics.

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Section: Discussionmentioning

confidence: 98%

Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats

Song

Wang

et al. 2017

Sci Rep

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“…This process was slightly modified from that used in our previous study (Gao et al, 2015). Then, 300 μL of a methanol solution was added to the sample to precipitate the proteins and 5 μg/mL of L-2-chlorophenylalanine within the methanol solution To validate the stability of the system, the QC samples were randomized in sequence.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The stability was then assessed based on the clustering of the QC samples in a principal component analysis (PCA) score plot of all the tested samples. This process was slightly modified from that used in our previous study (Gao et al, 2015).…”

Section: Uhplc-q-tof/ms Profiling Analysismentioning

confidence: 99%

Urinary metabolomics of complete Freund's adjuvant‐induced hyperalgesia in rats

Liu

Chen

et al. 2017

Biomedical Chromatography

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The aim of this study was to demonstrate the differences of metabolomics changes in a hyperalgesia model and find potent biomarkers of hyperalgesia. Seven rats were placed in metabolic cages. An emulsion containing 500 μg of Complete Freund's adjuvant (CFA) was used to induce hyperalgesia. Urine samples were collected prior to the injection of CFA and on post-injection days 1, 3 and 7. Ultraperformance liquid chromatography, coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS), was used for a quantitative analysis of urinary metabolic changes in the CFA-induced hyperalgesia model. Differences between the metabolic profiles of the rats in the four groups were analyzed using partial least squares discriminant analysis. Thirty-four potential urine metabolite biomarkers were identified, which changed in a trend similar to the pain threshold. These potential biomarkers were involved in 11 metabolic pathways, as follows: alanine, aspartate, and glutamate metabolism; ascorbate and aldarate metabolism; glycerolipid metabolism; glycerophospholipid metabolism; histidine metabolism; phenylalanine metabolism; sphingolipid metabolism; tryptophan metabolism; tyrosine metabolism; valine, leucine and isoleucine biosynthesis; and vitamin B6 metabolism. These results may improve our understanding of hyperalgesia and provide a basis for the clinical diagnosis of hyperalgesia.

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“…Moreover, CSH was used as one of the predominant ingredients in “Xueniao'an granules,” which was a very popular traditional Chinese medicine prescription for the treatment of urolithiasis. Pharmacological studies have shown that CSH has antimicrobial, antioxidant, hepatoprotective, antigenotoxic, antiplasmodial, cytotoxic, cardioactive, antidiabetic and anti‐inflammatory activities . Polymeric phenolic acids, flavonoids, diterpenes, triterpenes, essential oils and polysaccharides have been isolated from CSH by phytochemical approaches .…”

Section: Introductionmentioning

confidence: 99%

“…shown that CSH has antimicrobial, antioxidant, hepatoprotective, antigenotoxic, antiplasmodial, cytotoxic, cardioactive, antidiabetic and anti-inflammatory activities. [2][3][4][5][6] Polymeric phenolic acids, flavonoids, diterpenes, triterpenes, essential oils and polysaccharides have been isolated from CSH by phytochemical approaches. [7][8][9][10][11] Published literature concerning CSH has mainly focused on the quantification of several phenolic acids or flavonoids by high-performance liquid chromatography-photodiode array detector method or high-performance liquid chromatography/ tandem mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Characterization of polymeric phenolic acids and flavonoids in Clerodendranthi Spicati Herba using ultrahigh‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry with target and nontarget data mining strategy

Luo

Wen

Lai

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale Clerodendranthi Spicati Herba (CSH) is often used to treat urinary stones, urinary tract infections and nephritis in China. Much literature has reported that polymeric phenolic acids and flavonoids are the major bioactive components in CSH. Therefore, it is very meaningful to identify the polymeric phenolic acids and flavonoids in CSH. Methods Ultrahigh‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry (UHPLC/QTOF‐MS/MS) analysis with target and nontarget data mining strategy was proposed to rapidly profile the polymeric phenolic acids and flavonoids in CSH. Diagnostic product ions and neutral loss filter were beneficial for identifying the polymeric phenolic acids and flavonoids from complex compounds in CSH. Results A total of 118 compounds, including 85 polymeric phenolic acids and 33 flavonoids, were reasonably identified in CSH by comparing their main fragmentation pathways with literature data, and 85 of them were discovered in CSH firstly by nontarget analysis. Nine potential compounds were characterized tentatively as new pentameric and hexameric phenolic acids in CSH. Six types of polymeric phenolic acids (monomer, dimer, trimer, tetramer, pentamer and hexamer) and four types of flavonoids (apigenin, kaempferol, luteolin and quercetin) were identified in CSH. Conclusions The results indicated that the UHPLC/QTOF‐MS/MS method coupled with target and nontarget data mining strategy was feasible and rational for identifying the polymeric phenolic acids and flavonoids in complex chemical constituents of CSH. The findings will be conducive to the discovery of the active ingredients of CSH and the establishment of quality standards.

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Urinary metabonomics elucidate the therapeutic mechanism of Orthosiphon stamineus in mouse crystal-induced kidney injury

Cited by 27 publications

References 37 publications

Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats

Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats

Urinary metabolomics of complete Freund's adjuvant‐induced hyperalgesia in rats

Characterization of polymeric phenolic acids and flavonoids in Clerodendranthi Spicati Herba using ultrahigh‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry with target and nontarget data mining strategy

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