Urinary modified nucleosides as tumor markers in cancer of the urinary organs or female genital tract

Abstract

Using a sensitive and specific method involving high-performance liquid chromatography, urinary levels of four modified nucleosides--pseudouridine (psi), 1-methylinosine (m1I), 1-methyladenosine (m1A), and 1-methylguanosine (m1G)--were investigated before and after treatment in 31 patients with cancer of the urinary organs or the female genital tract. Before treatment m1I was the most frequently elevated nucleoside (77%). Pretreatment urinary levels of psi, m1I, and m1A in patients with stage 2-4 cancer of the female genital tract were significantly elevated compared to human healthy volunteers (p less than 0.005). Compared with the other nucleosides, psi appeared to correlate more closely with the clinical outcome (progression or regression) of patients with cancer of the female genital tract. In the case of patients with cancer of the urinary organs, m1I followed the clinical outcome better than the other nucleosides measured. Therefore psi and m1I seem to be useful for monitoring genito-urinary cancers.

Study of urinary nucleosides as biological marker in cancer patients analyzed by micellar electrokinetic capillary chromatography

“…It is observed that the results are similar to the situation by using 13 urinary nucleosides. These results are in agreement with the suggestions of several previous studies [9,17,24,25] although the previous samples were from German. On the other hand, attention should be paid to the alterations of the modified nucleosides under various catabolic conditions, e.g., infections, malnutrition, cardiac cachexia, and endocrine abnormalities.…”

Urinary nucleosides

“…Levels of ⌿, m 1 I, m 1 A, and m 1 G were determined before and after treatment in 31 patients with cancer of the urinary organs or the female genital tract (Koshida et al, 1985 4 C, and ⌿ were measured in the urine of 7 patients with ovarian cancer (Oerlemans & Lange, 1986). The patients were divided into three groups: benign, borderline, and malignant-based on histological criteria.…”

Reversed-phase high-performance liquid chromatographic investigation of urinary normal and modified nucleosides of cancer patients

“…cleosides with carcinogenesis [1,2] and proposed them as potential tumor markers [1,[3][4][5][6][7][8][9][10][11][12][13][14][15]. RNA contains modified nucleosides synthesized Although a capillary electrophoretic method depost-transcriptionally by modification enzymes.…”