Urinary N-acetyl- beta-D-glucosaminidase activities in patients with renal disease.

Wellwood, J. M.; Ellis, B.G.; Price, Robert G.; Hammond, Kimmarie; Thompson, A. E.; Jones, N. F.

doi:10.1136/bmj.3.5980.408

Cited by 184 publications

(76 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Enzymuria depends on the place and intensity of damage. Increased enzyme activity is a reflection of disease activity and residual functional capacity of kidney (2). Perturbation in renal tubules initially affects lysosomal membrane system in cells, resulting in increased loose of enzyme in the urine in early stadium.…”

Section: Introductionmentioning

confidence: 99%

Symmetric dimethyl arginine and N-acetyl- β-D-glucosaminidase lysosimyria of proximal renal tubules as a target for nephrotoxicity in patients with rheumatoid arthritis treated with disease modifying antirheumatic drugs.

Spasovski¹,

Latifi²,

Marina³

et al. 2013

J Nephropathol

View full text Add to dashboard Cite

Background: The aim of this study was to determine the effect of initial therapy with some disease modifying antirheumatic drugs (DMARDs) (Methotrexate and Ketoprofen) on glomerular and tubular integrity in patients with Rheumatoid arthritis (RA). Objectives: To determine whether there is a change in clinical and laboratory indicators of renal function in course of the follow up of treatment and whether that change correlates with the dynamics of the quantity of enzymes excreted in urine and reactants of the acute phase. Materials and Methods: Using colorimetric method for determination of NAG, samples of 70 participants were examined (35 RA patients treated with Ketoprofen only, 35 RA patients treated with combined use of Methotrexate and Ketoprofen). The follow up was 5 time-intervals in the course of 24 weeks. Results: There was moderate correlation between NAG and microalbuminuria (r=0,34) in the group of patients treated with Ketoprofen only, while statistically significant correlation (r=0,21) was seen in group of patients with combined use of Methotrexate and Ketoprofen. NAG enzymuria in size, number of patients registered, and time of appearance were greater and appears earlier in the group with the combined use of Methotrexate and Ketoprofen compared with the mono-therapy with Ketoprofen. Mean urinary NAG induction was increasing with the concomitant use of Methotrexate and Ketoprofen. Conclusions: Methotrexate is more potent NAG inductor than Ketoprofen and provokes greater tubular enzymuria than Ketoprofen. www.nephropathol.com DOI:10.5812/nephropathol.8989 J Nephropathology. 2013; 2(1): 36-52 Original Article Journal of NephropathologyImplication for health policy/practice/research/medical education: Determination of the urinary N-acetyl-β-D-glucosaminidase together with the urinary creatinine excretion could serve as a more sensitive test for renal lesions in patients suffering from rheumatoid arthritis, as an additional diagnostic tool, and the information for the status of the disease.

show abstract

Section: Introductionmentioning

confidence: 99%

Symmetric dimethyl arginine and N-acetyl- β-D-glucosaminidase lysosimyria of proximal renal tubules as a target for nephrotoxicity in patients with rheumatoid arthritis treated with disease modifying antirheumatic drugs.

Spasovski¹,

Latifi²,

Marina³

et al. 2013

J Nephropathol

View full text Add to dashboard Cite

show abstract

“…The urinary excretion of various constitutive proteins from kidney cells as markers of kidney injury has been explored in the past using multiple enzymes such as N-acetyl-b-D-glucosaminidase (NAG), 2 c glutamyltranspeptidase (GGT) 3 and lactate dehydrogenase (LDH). 4 Several of these have been shown to be elevated after SWL in animal models, but have been found to be inconsistent and less reliable in human studies.…”

Section: Introductionmentioning

confidence: 99%

Urinary Expression of Novel Tissue Markers of Kidney Injury After Ureteroscopy, Shockwave Lithotripsy, and in Normal Healthy Controls

Fahmy

Şener

Sabbisetti

et al. 2013

Journal of Endourology

View full text Add to dashboard Cite

Background and Purpose: Shockwave lithotripsy (SWL) and ureteroscopy (URS) are minimally invasive treatment alternatives for kidney stones. Although less invasive, SWL subjects the renal parenchyma to a high level of energy and the potential to cause renal injury. The ability to detect renal injury post-SWL in a reliable and noninvasive way would be clinically beneficial. Kidney injury molecule 1 (KIM-1) and N-acetyl-b-Dglucosaminidase (NAG) are two proteins secreted by the kidney into the urine and have been found to be sensitive markers of acute kidney injury in transplant patients. The aim of this work was to measure urinary levels of KIM-1 and NAG in patients with kidney stone who were treated by SWL or URS and in nonstone volunteers. Patients and Methods: Patients with kidney stones who were treated by SWL (n = 50) or URS (n = 10) were recruited. Voided urine samples were collected before and 2 to 3 hours after URS and SWL. In addition, further urinary specimens were collected 2 days and 2 weeks post-SWL treatment. Voided urine samples from healthy volunteers were also collected. Results: Mean KIM-1 values were increased in patients with kidney stones when compared with volunteers. KIM-1 and NAG levels significantly increased post-SWL and returned to baseline within 2 weeks post-SWL. Poor kidney function was significantly associated with increased biomarker activity both in baseline and post-SWL measurements. There was no significant change in urinary KIM-1 and NAG concentrations before and after URS. Conclusions: Kim-1 and NAG levels significantly increased post-SWL treatment suggesting a potential role for these urinary markers in identifying patients at higher risk of tissue injury.

show abstract

“…Urinary creatinine concentration (mmol/K) was determined using the Bonsens and Taussky method [12] based on the Jaffe reaction [13] . The determination of creatinine was carried out in order to correct for variations caused by change in urine flow [14] . AAP activity (u/mol Cr) was carried out according to the method of Mattenheimer et al [15] .…”

Section: Methodsmentioning

confidence: 99%

Urinary Enzymes and Microalbuminuria as Indicators of Renal Nephropathy in Saudi Patients

Lary¹

2008

sci

View full text Add to dashboard Cite

Abstract. In the current work we tried to investigate some new biochemical markers in the urine of kidney patients, kidney transplant recipients and control subjects of the Saudi population, such markers are N-acetyl-β-D-glucosaminidase (NAG), Alkaline Phosphatase (ALP), Alanine Aminopeptidase (AAP) as well as microalbuminuria. Kidney patients, males and females, age ranged from 16-85 years, and kidney transplant recipients, male and female, age ranged from 18-75 years.Results from control subjects showed a slight elevation of total urinary protein and NAG in females. This may be due to the higher level of creatinine concentration in male subjects, that would result in lower protein concentration and NAG activity when expressed in terms of creatinine. Microalbuminuria, glucose and pH results were in close agreement with the published data and no sex differences were recorded when compared with the males. There were statistically significant differences in most of parameters investigated between kidney patients and the normal population except for glucose level and pH value. The reduction in creatinine concentration may reflect changes in the glomerular filtration rate (GRF). Higher levels of urinary NAG activity were associated with high microalbuminuria and raised AAP levels.Increase urinary NAG predominantly reflects decreased renal function and tubular damage. ALP increased levels reflect a lesion of the proximal convoluted tubules and/or intensive degeneration of the tubular epithelial cells. The increase in NAG activity correlates well with urinary protein and may indicate an increase in activity of renal tubular cells. Kidney transplant recipients showed a significant increase in NAG, ALP and AAP enzymes compared to control subjects. The marked increase in most parameters may indicate that patients are in an immediate functioning graft.

show abstract

Urinary N-acetyl- beta-D-glucosaminidase activities in patients with renal disease.

Cited by 184 publications

References 5 publications

Symmetric dimethyl arginine and N-acetyl- β-D-glucosaminidase lysosimyria of proximal renal tubules as a target for nephrotoxicity in patients with rheumatoid arthritis treated with disease modifying antirheumatic drugs.

Symmetric dimethyl arginine and N-acetyl- β-D-glucosaminidase lysosimyria of proximal renal tubules as a target for nephrotoxicity in patients with rheumatoid arthritis treated with disease modifying antirheumatic drugs.

Urinary Expression of Novel Tissue Markers of Kidney Injury After Ureteroscopy, Shockwave Lithotripsy, and in Normal Healthy Controls

Urinary Enzymes and Microalbuminuria as Indicators of Renal Nephropathy in Saudi Patients

Contact Info

Product

Resources

About