Urinary Podocyte Biomarkers and Glomerular Histologic Change

Inoue, Kazunori

doi:10.34067/kid.0008212021

Cited by 4 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge few studies have assessed both these markers (PDC/CR, NEP/CR ratio) simultaneously in patients with GN. A previous study [22] assessed the impact of systemic lupus erythematosus (SLE) on urinary levels of podocalyxin as well as its correlation to renal biopsy histological parameters, proteinuria, and disease activity. They found a significant correlation of the estimated ratios with the lupus nephritis class as well as with the BILAG scores (especially with PDC/CR ratio).…”

Section: Discussionmentioning

confidence: 99%

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Giannou,

Gakiopoulou,

Stambolliu

et al. 2024

Preprint

View full text Add to dashboard Cite

Introduction: Glomerulopathy is a term used to describe a broad spectrum of renal diseases, character-ized by dysfunction of glomerular filtration barrier, especially of podocytes. Several podocyte-associated proteins have been found and proved their usefulness as urine markers of podocyte dysfunction. Two of them are nephrin (NEP) and prodocalyxin (PDC). Purpose: This study aims to evaluate the association of podocyte damage, as it is demonstrated via the concentrations of urinary proteins, with clinical and histological data from patients with several types of glomerulonephritis. Methods: We measured urine levels of two podocyte-specific markers, NEP and PDC (corrected for urine creatinine levels), in patients with a wide range of glomerulopathies. Serum and urine parameters as well as histological parameters from renal biopsy were recorded. Results: In total, data from 37 patients with glomerulonephritis and 5 healthy controls were analyzed. PDC and NEP concentrations correlated between them and with serum creatinine levels (p=0.001 and p=0.013 respectively), and with histological lesions associated with chronicity index of renal cortex, such as severe interstitial fibrosis, severe tubular atrophy and hyalinosis (for PDC/NEP, all p3gr/24h with diffuse fusion of podocyte foot processes (p=0.016) was identified. Conclusions: Podocalyxin and nephrin concentrations in urine represent markers of podocyte dysfunction and in our study they were associated both with serum creatinine and histological chronicity indices.

show abstract

Section: Discussionmentioning

confidence: 99%

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Giannou,

Gakiopoulou,

Stambolliu

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…They found a significant correlation between the estimated ratios, the lupus nephritis class and the BILAG scores (especially with the PDC/CR ratio). Another study also found that podocalyxin is associated with GN disease activity [ 21 ]. However, our study highlights that the increased urine levels of PDC and NEP are mainly associated with chronicity indices such as tubular atrophy, severe fibrosis, and GBM thickening not only in podocytopathies, but also interestingly in non-podocytopathies.…”

Section: Discussionmentioning

confidence: 99%

Urine Nephrin and Podocalyxin Reflecting Podocyte Damage and Severity of Kidney Disease in Various Glomerular Diseases—A Cross-Sectional Study

Giannou,

Gakiopoulou,

Stambolliu

et al. 2024

JCM

View full text Add to dashboard Cite

Background/Objectives: Glomerulopathy is a term used to describe a broad spectrum of renal diseases, characterized by dysfunction of glomerular filtration barrier, especially of podocytes. Several podocyte-associated proteins have been found and proved their usefulness as urine markers of podocyte dysfunction. Two of them are nephrin (NEP) and prodocalyxin (PDC). This study aims to evaluate the association of podocyte damage, as it is demonstrated via the concentrations of urinary proteins, with clinical and histological data from patients with several types of glomerulonephritis. Methods: We measured urine levels of two podocyte-specific markers, NEP and PDC (corrected for urine creatinine levels), in patients with a wide range of glomerulopathies. Serum and urine parameters as well as histological parameters from renal biopsy were recorded. Results: In total, data from 37 patients with glomerulonephritis and 5 healthy controls were analyzed. PDC and NEP concentrations correlated between them and with serum creatinine levels (p = 0.001 and p = 0.013 respectively), and with histological lesions associated with chronicity index of renal cortex, such as severe interstitial fibrosis, severe tubular atrophy and hyalinosis (for PDC/NEP, all p < 0.05). In addition, the PDC and NEP demonstrated statistically significant correlations with interstitial inflammation (p = 0.018/p = 0.028). Regarding electron microscopy evaluation, PDC levels were correlated with distinct characteristics, such as fibrils and global podocyte foot process fusion, whereas the NEP/CR ratio was uniquely significantly associated with podocyte fusion only in non-immune-complex-mediated glomerulonephritis (p = 0.02). Among the other clinical and histological parameters included in our study, a strong correlation between proteinuria >3 g/24 h and diffuse fusion of podocyte foot processes (p = 0.016) was identified. Conclusions: Podocalyxin and nephrin concentrations in urine are markers of podocyte dysfunction, and in our study, they were associated both with serum creatinine and histological chronicity indices.

show abstract

“…Shoji et al (2016) dalam penelitiannya melaporkan korelasi positif yang signifikan antara rasio kreatinin mikroalbumin urin dan podokaliksin dimana tingkat podocalyxin urine meningkat secara bertahap dengan derajat nefropati diabetik (p<0,029) dan kadar podokaliksin urin berkorelasi positif dengan rasio mikroalbumin/ kreatinin urin (UM/CR) r=0,227; p=0,002). Pengukuran molekul podosit dalam sampel urin dapat berfungsi sebagai penanda spesifik kerusakan sel podosit ginjal (Inoue, 2022). Penelitian yang dilakukan oleh Asao et al (2012) mengungkapkan korelasi yang signifikan antara kadar podocalyxin urin dan tingkat kerusakan ekstrakapiler akut (r=0,72; p<0,001) tetapi tingkat ekskresi protein tidak berkorelasi dengan gangguan glomerulus akut.…”

Section: Pembahasanunclassified

Podocalyxin Urine sebagai Biomarker Prediktor Nefropati Diabetik

Orno,

Pelu

2023

Health Inf J Penelit

View full text Add to dashboard Cite

Diabetes melitus tipe 2 (DMT2) merupakan penyebab terbanyak dari diabetes, terdiri atas sekitar 85% kasus. Nefropati Diabetik (ND) merupakan salah satu komplikasi dari penyakit diabetes melitus yang dapat berakhir sebagai gagal ginjal. Podocalyxin (PDX) merupakan protein yang diekspresikan dalam podosit ginjal yang berperan dalam menjaga struktur glomeruli. Desain penelitian ini dilakukan dengan metode observasional analitik dengan desain cross sectional. Jumlah sampel sebanyak 25 subjek DMT2 dengan ND dan 25 subjek DMT2 tanpa ND yang diperoleh dengan teknik sampling probability purposive sampling. Penelitian ini dilakukan di Poliklinik Endokrin, Laboratorium Patologi Klinik, Laboratorium Hasanuddin University Medical Research Center (HUM-RC) Rumah Sakit Universitas Hasanuddin Makassar. Subjek DMT2 dengan dan tanpa ND dikategorikan berdasarkan kadar Albumin/Creatinine Ratio (ACR) yang diukur menggunakan metode imunoturbidimetri dan kadar podocalyxin urine diukur menggunakan metode Enzyme-linked Immunosorbent Assay. Hasil penelitian menunjukkan Area Under Curve validitas PDX terukur pada 0,779 dengan derajat kepercayaan 0,685-0,913 (p<0,05). Nilai cut off point sebesar 0,175 ng/mL dengan sensitivitas sebesar 66,7%, spesifisitas sebesar 64,3%. Disimpulkan bahwa PDX dengan nilai kadar 0,175 ng/mL dapat digunakan sebagai biomarker prediktor ND.

show abstract

Urinary Podocyte Biomarkers and Glomerular Histologic Change

Cited by 4 publications

References 20 publications

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Urine Nephrin and Podocalyxin Reﬂecting Podocyte Damage and Severity of Kidney Disease in Various Glomerulonephritis. A Cross-Sectional Study

Urine Nephrin and Podocalyxin Reflecting Podocyte Damage and Severity of Kidney Disease in Various Glomerular Diseases—A Cross-Sectional Study

Podocalyxin Urine sebagai Biomarker Prediktor Nefropati Diabetik

Contact Info

Product

Resources

About