Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography

Abstract

Urinary renalase concentration is reduced in the aftermath of CA/PCI, which may be related to CI-AKI development. Further studies are warranted to elucidate the role of urinary renalase as a CI-AKI diagnostic marker.

A new method for quantitative determination of renalase based on mass spectrometric determination of a proteotypic peptide labelled with stable isotopes

“…According to the literature data, the median levels of urinary renalase determined in various groups of patients by ELISA were within the range 136–2843.6 ng/mg Cr. The median concentrations of urinary renalase determined in children of various ages varied from 136 ng/mg Cr (children above 15 years) to 407 ng/mg Cr (children under 3 years), while in adults (59–71 years) the median was 2843.6 ng/mg . These values significantly exceed the upper concentration of recombinant renalase used for generation of the calibration curve in our study.…”

“…The highest ( Figure 3A) estimated concentration was 75.84 pg/mL urine (or 758.4 pg/mg Cr) (sample 1), whereas the lowest estimated concentration detected by the method was 2.31 pg/mL (1.37 pg/mg) ( Figure 3B) while in adults (59-71 years) the median was 2843.6 ng/mg. 12 These values significantly exceed the upper concentration of recombinant renalase used for generation of the calibration curve in our study.…”

“…Addition of standard renalase solution to the blank urinary (renalase‐free) samples (final calculated renalase of 500 ng/mL mimicking its urinary concentration available in the literature) followed by ammonium sulfate precipitation and subsequent mass spectrometric determination revealed a recovery rate of 71.95 ± 8.73%.…”

“…It is secreted by the kidney and kidney‐derived cell cultures, and renalase deficiency frequently develops in patients with end‐stage renal disease (ESRD), patients with other chronic renal diseases and related experimental animal models, thus determining development of hypertension complications. There are many reports of altered levels of renalase in biological body fluids (plasma and urine) in these and other groups of patients (or animal models) . However, there is certain inconsistency in results obtained in different laboratories.…”

“…There are many reports of altered levels of renalase in biological body fluids (plasma and urine) in these and other groups of patients (or animal models). 2,[8][9][10][11][12] However, there is certain inconsistency in results obtained in different laboratories. For example, Western blot analysis of plasma samples from rats that underwent removal of five sixths of kidney tissue revealed a significant decrease in the plasma renalase level.…”